中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1449-1453.doi: 10.3969/j.issn.1673-8225.2011.08.029

• 生物材料综述 biomaterial review • 上一篇    下一篇

微囊化细胞的低温保存

叶  萍1，2，常兆华1，2，刘宝林1，2，赵庆孝1，2，彭承宏3，4，沈柏用3，4，韩宝三3，4，5   

  1. 上海理工大学，1制冷与低温研究所，2医疗器械与食品学院生物热科学研究所，上海市 200093；上海交通大学医学院附属瑞金医院，3普外科， 5乳腺疾病诊治中心，上海市 200025；4上海消化外科研究所，上海市 200025
  • 收稿日期:2010-08-24 修回日期:2010-09-25 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 韩宝三，博士，博士后，副主任医师，中国抗癌协会乳腺癌专业委员会青年委员，中国光学学会生物医学光子学委员会青年委员，上海交通大学医学院附属瑞金医院普外科、乳腺疾病诊治中心，上海市 200025；上海消化外科研究所，上海市 200025 hanbaosan@126.com
  • 作者简介:叶萍☆，男，1981年生，上海市崇明县人，汉族，上海理工大学在读博士，主要从事低温生物医学相关器械和方法学的研究。 iamyeping@163.com
  • 基金资助:

    国家“十一五”高新技术研究发展计划－“863”项目（2008AA02Z417）；国家自然科学基金项目（30801107）；国家自然科学基金项目（30772105）；国家自然科学基金项目（30672043）；2005/2008国家自然科学基金重点项目（20434030）；国家自然科学基金项目（20074031）；上海市教委科研创新项目（09YZ84）；上海市科委自然科学基金项目（07ZR14076）；上海市科委自然科学基金项目（09140902300）；山东省科技攻关计划（2008GG10002027）；上海市研究生创新基金项目（JWCXSL1021）。

Cryopreservation of microencapsulated cells

Ye Ping1,2, Chang Zhao-hua1,2, Liu Bao-lin1,2, Zhao Qing-xiao1,2, Peng Cheng-hong3,4, Shen Bai-yong3,4, Han Bao-san3,4,5   

  1. 1Institute of Refrigeration and Cryogenics, 2Institute of Biology and Thermal Science, College of Medical Devices and Food, University of Shanghai for Science and Technology, Shanghai  200093, China; 3Department of General Surgery, 5Breast Disease Diagnosis and Treatment Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai   200025, China; 4Shanghai Institute of Digestive Surgery, Shanghai  200025, China
  • Received:2010-08-24 Revised:2010-09-25 Online:2011-02-19 Published:2011-02-19
  • Contact: Han Bao-san, Doctor, Associate chief physician, Department of General Surgery, Breast Disease Diagnosis and Treatment Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Shanghai Institute of Digestive Surgery, Shanghai 200025, China hanbaosan@126.com
  • About author:Ye Ping☆, Studying for doctorate, Institute of Refrigeration and Cryogenics, Institute of Biology and Thermal Science, College of Medical Devices and Food, University of Shanghai for Science and Technology, Shanghai 200093, China iamyeping@163.com
  • Supported by:

    the National “Eleventh Five-Year” High-Tech Research and Development Program (863 Project), No.2008AA02Z417*; the National Natural Science Foundation of China, No. 30801107*, 30772105*, 30672043*, 20074031*; Key Program of the National Natural Science Foundation of China, No. 20434030*; Scientific Research and Innovation Project of Shanghai Municipal Education Department, No. 09YZ84*; Natural Science Foundation of Shanghai Science and Technology Commission, No. 07ZR14076*, 09140902300*; Science and Technology Research Program of Shandong Province, No. 2008GG10002027*; Graduate Innovation Fund of Shanghai, No. JWCXSL1021*

PDF

285

被引次数

3

摘要：

背景：微胶囊是一种有效的免疫隔离工具，低温冷冻方便了微囊的保存与运输，有利于微囊化技术在临床的推广应用。
目的：综述微囊低温保存技术中微囊低温保存特点、不同保存方法优缺点、研究方法及微囊低温保存实验现状。
方法：检索1980/2010 PubMed数据库，1991/2010万方数据库、维普数据库有关微囊低温保存研究，低温保存工艺理论及专用仪器，微囊低温保存技术医学应用的文献。
结果与结论：微囊化细胞在低温保存过程中的损伤特点与细胞、组织有很大不同。微囊相对较大的尺寸与复杂的囊壁半透膜结构，使得微囊结构与囊内细胞更容易受到溶质损伤与冰晶损伤，因此不能简单套用单细胞悬液的低温保存方案。慢速冷却法与玻璃化法是两种常见的微囊化细胞低温保存方法，各有利弊。微囊化细胞低温保存技术尚未成熟，在开发新型微囊低温保存设备，优化设计微囊低温保存方案的同时，需进一步加强对微囊冻存机制更深层次的探索。

关键词: 低温保存, 微囊化细胞, 玻璃化, 移植, 胰岛移植, 生物材料

Abstract:

BACKGROUND: Microcapsule is an effective tool for immunoisolation, cryopreservation makes the preservation and the transportation of the microcapsules easy, which is helpful for microencapsulated technology applied in clinical practice.
OBJECTIVE: To summarize the cryopreservation of microencapsulated cells, such as cryopreservation characteristic of microencapsulated cells, advantages and disadvantages of different cryopreservation methods, research methods and so on.
METHODS: A computer-based online search of PubMed database (from 1980 to 2010), Wanfang database (from 1991 to 2010) and Vip database (from 1991 to 2010) was performed for articles related to the cryopreservation of microencapsulated cells, cryopreservation technology and devices, cryopreservation application in medical field.
RESULTS AND CONCLUSION: The cryodamage characteristic of microencapsulated cells are different to the cells and tissues. The relatively large size of microcapsules and complex structure of semi-permeable membrane make it susceptibly that microcapsule structure and cells in microcapsules are damaged by ice crystals and solution in cryopreservation. So the cryopreservation protocol of cell suspension could not be used in microencapsulated cells cryopreservation. Slow-cooling and vitrification are two common methods of the cryopreservation of microencapsulated cells, each has advantages and disadvantages. The technology of cryopreservation of microencapsulated cells is not yet mature. The mechanism underlying the cryopreservation of microencapsulated cells needs further strengthen at the same time of exploring the optimal cryopreservation protocols for microencapsulated cells and new cryopreservation facilities for microencapsulated cells.

中图分类号: