中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1360-1364.doi: 10.3969/j.issn.1673-8225.2011.08.007

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

纳米羟基磷灰石纤维蛋白凝胶复合重组人成骨蛋白1修复骨缺损

赵玉驰1，王大平2，赖苑威1，熊建义2，王力刚1，朱伟民2，黄仁辉1，袁  杰1   

  1. 1深圳市盐田区人民医院，广东省深圳市 518000
    2深圳市第二人民医院，广东省深圳市 518035
  • 收稿日期:2010-08-30 修回日期:2011-01-01 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 王大平，主任医师，深圳市第二人民医院，广东省深圳市 518035 szhzwm@126.com
  • 作者简介:赵玉驰，男，1959年生，湖南省邵阳市人，汉族，1983年衡阳医学院毕业，教授，硕士生导师，主要从事创伤骨科研究。 westerlife@126.com
  • 基金资助:

    深圳市科技计划项目资助。

Nano-hydroxyapatite/fibrin glue/recombinant human osteogenic protein-1 for repair of bone defects

Zhao Yu-chi1, Wang Da-ping2, Lai Yuan-wei1, Xiong Jian-yi2, Wang Li-gang1, Zhu Wei-min2, Huang Ren-hui1, Yuan Jie1   

  1. 1Shenzhen Yantian People’s Hospital, Shenzhen  518000, Guangdong Province, China
    2Shenzhen Second People’s Hospital, Shenzhen  518035, Guangdong Province, China
  • Received:2010-08-30 Revised:2011-01-01 Online:2011-02-19 Published:2011-02-19
  • Contact: Wang Da-ping, Chief physician, Shenzhen Second People’s Hospital, Shenzhen 518035, Guangdong Province, China szhzwm@126.com
  • About author:Zhao Yu-chi, Professor, Master’s supervisor, Shenzhen Yantian People’s Hospital, Shenzhen 518000, Guangdong Province, China westerlife@126.com
  • Supported by:

    Shenzhen Science and Technology Research Project, No. 200533118*

PDF

345

被引次数

9

摘要：

背景：纳米级的羟基磷灰石纤维蛋白凝胶材料与人体内组织成分更为相似，具有良好的生物与力学性能，但缺乏骨诱导作用。
目的：观察纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1复合人工骨的骨缺损修复能力。
方法：制备新西兰大白兔单侧桡骨缺损模型后，以数字表法随机分为3组，分别植入不同材料行骨缺损修复：纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1人工骨组、纳米羟基磷灰石/纤维蛋白凝胶组、空白对照组(未植入任何材料)。术后4，8，12周行大体标本观察、X射线、扫描电镜、放射性核素骨扫描及生物力学测试，比较各组材料修复骨缺损的能力。
结果与结论：术后4，8，12周，纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1人工骨组X射线评分、成骨效果、放射性核素聚集强度、生物力学强度均高于纳米羟基磷灰石/纤维蛋白凝胶组(P < 0.05)。空白对照组骨缺损区无骨性连接，骨端硬化，骨缺损未能修复。说明纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1复合人工骨具有良好的骨缺损修复能力，有望成为一种理想的骨缺损修复材料。

关键词: 纤维蛋白凝胶, 羟基磷灰石, 重组人成骨蛋白1, 人工骨, 骨缺损

Abstract:

BACKGROUND: The nano-hydroxyapatite/fibrin glue (Nano-HA/FG) is similar to the human tissues and possesses good biological and mechanical properties, but it lacks of bone induction effect.
OBJECTIVE: To investigate the repair capacity of nano-hydroxyapatite/fibrin glue/recombinant human osteogenic protein-1 (Nano-HA/FG/rhOP-1) on bone defects.
METHODS: Animal models of bone defects were established on the bilateral radius of New Zealand white rabbits, which were randomly divided into Nano-HA/FG/rhOP-1 artificial bone group (bone defect was repaired with artificial bone), Nano-HA/FG artificial bone group (bone defect was repaired with Nano-HA/FG artificial bone) and blank control group (bone defect was left unrepaired). The ability of repairing bone defect was evaluated by gross specimen observation, X-ray radiograph, scanning electronic microscope, radionuclide bone scan, and biomechanical test at 4, 8, 12 weeks after operation.
RESULTS AND CONCLUSION: Radiographical scores, osteogenic effect, aggregation intensity of radionuclide, and biomechanical strength in Nano-HA/FG/ rhOP-1 artificial bone group were higher than that in Nano-HA/FG group (P < 0.05) at 4, 8, 12 weeks after operation. There was no bone connection in the defect field, bone end sclerosis, and the bone defect was not repaired in the blank control group. It suggested that Nano-HA/FG/ rhOP-1 artificial bone has good repair capacity of bone defects and is expected to be an ideal repairing material for bone defect.

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