中国组织工程研究

中国组织工程研究

• 生物材料学术探讨 biomaterial academic discussion • 上一篇    下一篇

组织工程肌腱材料修复肌腱损伤

李  昂1，戴  艳2，涂相仁1   

  1. 1江西教育学院体育系，江西省南昌市 330029；2江西师范大学体育运动技术学院，江西省南昌市 330006
  • 出版日期:2010-12-17 发布日期:2010-12-17
  • 作者简介:李昂，男，1977年生，江西省南昌市人，汉族，2003年江西科技师范学院毕业，讲师，主要从事体育教育学研究。 zcqaerobics@126.com

Tissue engineered tendon materials for tendon damage repair

Li Ang1, Dai Yan2, Tu Xiang-ren1   

  1. 1Department of Physical Education, Jiangxi Institute of Education, Nanchang   330029, Jiangxi Province, China; 2Sports Technology Institute, Jiangxi Normal University, Nanchang   330006, Jiangxi Province, China
  • Online:2010-12-17 Published:2010-12-17
  • About author:Li Ang, Lecturer, Department of Physical Education, Jiangxi Institute of Education, Nanchang 330029, Jiangxi Province, China zcqaerobics@126.com

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摘要：

目的：总结和分析组织工程肌腱研究进展，在此基础上进一步分析组织工程肌腱作为替代物对于肌腱损伤修复的可能性，以及对未来发展进行展望。
方法： 以“组织工程，种子细胞，肌腱，损伤修复，支架材料”为中文关键词，以“tissue engineering，seed cells，tendon，damage repair，scaffold”为英文关键词，应用计算机检索1994-01/2009-12 Pubmed数据库(http://www.ncbi.nlm. nih.gov/PubMed)及维普数据库(http://www.cqvip.com/)有关运动性肌腱损伤与组织工程肌腱的文献，排除重复研究或Meta分析类文章。共纳入25篇文献，重点对以下4个问题进行讨论：①运动性肌腱工程生物力学研究进展。②肌腱组织工程种子细胞的分类与筛选。③肌腱组织工程支架材料应具备的特点。④组织工程肌腱面临的问题与研究方向。
结果：目前，在肌腱组织工程中研究的种子细胞有肌腱细胞、皮肤成纤维细胞和骨髓基质干细胞。标准种子细胞的难以确立一直制约着组织工程的发展，如何获得大规模、再生性的种子细胞是当前组织工程化肌腱研究面临的最为关键的问题。组织工程化人工肌腱修复缺损肌腱，与其他传统方法相比，主要具有以下优点：①所形成的肌腱组织有活力和功能，可对肌腱缺损进行形态修复和功能重建，并达到永久性替代。②以相对少量的肌腱细胞经体外培养扩增后，修复严重的肌腱缺损。③按缺损肌腱形态任意塑形，达到形态修复。
结论：组织工程肌腱要真正应用于临床，关键是如何模拟体内环境，在体外成功构建肌腱组织，因而在体外模拟体内环境进行组织工程的构建是未来的研究方向。

关键词: 组织工程肌腱, 种子细胞, 支架材料, 损伤修复, 生物力学

Abstract:

OBJECTIVE: To summarize and analyze the research progress of tissue engineered tendon, and further analyze the possibility of tissue engineered tendon as a substitute for tendon repair, as well as future development prospects.
METHODS: Using “tissue engineering, seed cells, tendons, damage repair, scaffold” in Chinese and English as the key words, a computer-based retrieval of PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and VIP database (http://www.cqvip.com/) from January 1994 to December 2009 was performed for articles on exercise-induced tendon injury and tissue engineered tendon, excluding Meta analysis or repeated research. A total of 25 articles were included, focusing on the following four aspects for discussion: ① biomechanical research progress of sport tendon engineering; ② classification and screening of tendon tissue engineering seed cells; ③ necessary characteristics of tendon tissue engineering scaffolds; ④ existing problems and research directions of tendon tissue engineering.
RESULTS: At present, the seed cells in tendon tissue engineering include tendon cells, skin fibroblasts and bone marrow stromal stem cells. Uncertain standards for seed cells has restricted the development of tissue engineering, how to get large-scale regenerative seed cells is the most critical issue of tissue engineered tendon studies currently facing. Tissue engineered artificial tendon is supervisor to other traditional methods for repairing tendon defects, mainly in the following advantages: ① the forme d tendon tissue has vitality and function, can reconstruct the morphology and function of the defective tendon, and achieve a permanent replacement; ② repair serious tendon defects after a relatively small amount of tendon cells culture and amplification in vitro; ③ molding defective tendon, to repair shape.
CONCLUSION: The key of tissue engineered tendon in practical clinical application is how to simulate the environment in vivo and to successfully construct tendon tissue in vitro, thus simulating in vivo environment and construction in vitro of tissue engineering is future research direction.

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