中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9253-.doi: 10.3969/j.issn.1673-8225.2010.49.030

• 干细胞基础实验 • 上一篇    下一篇

常氧培养与低氧培养肺间充质细胞凋亡的比较

邢文，杨少光，韩之波，茹永新，刘蒙，卢士红，赵钦军，李建平，任倩，韩忠朝   

  1. 中国医学科学院血液学研究所血液病医院，实验血液学国家重点实验室，天津市 300020
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 作者简介:邢文★，男，1969年生，山东省莱阳市人，汉族，2004年上海第二医科大学毕业，硕士，助理研究员，主要从事实验血液学方面的研究。xw2318@yahoo.com.cn 并列第一作者：杨少光，男，1953年生，重庆市人，汉族，1977年中山医科大学毕业，副主任技师，从事血液病相关造血干细胞和再造血微环境的研究。Yangsg666@163.com
  • 基金资助:

    教育部博士点基金新教师项目资助课题(20070023087)，课题名称：骨髓组织工程及其对同基因骨髓移植的支持作用研究。

Apoptosis of lung mesenchymal cells under normal oxygen culture versus low oxygen culture

Xing Wen, Yang Shao-guang, Han Zhi-bo, Ru Yong-xin, Liu Meng, Lu Shi-hong, Zhao Qin-jun, Li Jian-ping, Ren Qian, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin  300020, China
  • Online:2010-12-03 Published:2010-12-03
  • About author:Xing Wen★, Master, Assistant researcher, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, China xw2318@yahoo.com.cn Yang Shao-guang, Associate chief technician, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, China Yangsg666@163.com
  • Supported by:

     the New Teacher Program of Doctor Research Center of Ministry of Education, No. 20070023087*

PDF

347

被引次数

3

摘要：

背景：小鼠间充质细胞在体外培养扩增困难，细胞凋亡是否是其原因之一，目前未知。
目的：观察常氧和低氧条件下，小鼠肺间充质细胞的凋亡率是否有差别。
方法：用组织块培养法获得C57BL/6J雄性小鼠肺间充质细胞，分别在常氧(体积分数20%氧)和低氧(体积分数5%的氧)条件下培养，观察细胞内活性氧水平、凋亡率和超微结构的变化。
结果与结论：体积分数为5%和20%氧气培养的细胞随代数的增加造血细胞均逐渐减少。体积分数20%氧气培养的小鼠肺间充质细胞内的活性氧水平明显高于体积分数5%氧气培养的细胞。体积分数20%氧气培养的肺间充质细胞的凋亡现象较常见，体积分数5%氧气培养的细胞凋亡现象较少见。提示常氧培养条件下，细胞的凋亡率增加，降低氧浓度可减少细胞的凋亡率。

关键词: 氧, 间充质细胞, 组织块培养, 凋亡, 常氧培养, 低氧培养

Abstract:

BACKGROUND: Expansion of murine-derived mesenchymal cells is difficult in vitro and it is unclear whether this phenomenon is caused partly by cell apoptosis.
OBJECTIVE: To compare the differences of mouse lung mesenchymal cell apoptosis between normal oxygen and low oxygen culture conditions.
METHODS: C57BL/6J male mouse lung derived-mesenchymal cells were isolated by primary explant culture and cultured under normal (20% O2) and low oxygen (5% O2) conditions. The differences in intracellular active oxygen level, apoptotic rate and ultrastructure were observed.
RESULTS AND CONCLUSION: The number of hematopoietic cells was decreased with increased cell passage under both 5% and 20% O2 condition. The active oxygen levels were significantly greater in the mouse lung-mesenchymal cells under 20% O2 condition compared with those under 5% O2 condition. Apoptosis was more in cells under 20% O2 condition compared with those under 5% O2 condition. These indicate that under normal oxygen culture condition, the apoptotic rate of cells increases and low oxygen can reduce the apoptosis.

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