中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9216-.doi: 10.3969/j.issn.1673-8225.2010.49.022

• 干细胞与中医药 • 上一篇    下一篇

人参皂苷Re对K562细胞向红系分化的作用

雷翠蓉1，姜蓉1，王红宁1，何轩1，左国伟2，陈地龙1，官涛3，王建伟1   

  1. 1重庆医科大学组织胚胎教研室，干细胞与组织工程研究室，重庆市  400016；2重庆医科大学临床检验诊断学省部共建教育部重点实验室，重庆市 400016；3重庆医科大学生理教研室，重庆市 400016
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 王建伟，博士，教授，博士生导师，重庆医科大学干细胞与组织工程研究室，重庆市 400016 wjwcq@yahoo.com.cn
  • 作者简介:雷翠蓉★，女，1982年生，重庆市人，汉族，重庆医科大学组织胚胎学专业在读硕士，医师，主要从事中药药理及干细胞研究。 zhuozhi1014@163.com

Erythroid lineage differentiation of K562 cells induced by gensingnoside Re

Lei Cui-rong1, Jiang Rong1, Wang Hong-ning1, He Xuan1, Zuo Guo-wei2, Chen Di-long1, Guan Tao3, Wang Jian-wei1   

  1. 1Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering,  Chongqing Medical University, Chongqing  400016, China; 2Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing  400016, China; 3Department of Physiology, Chongqing Medical University, Chongqing 400016, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Wang Jian-wei, Doctor, Professor, Doctoral supervisor, Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China wjwcq@yahoo.com.cn
  • About author:Lei Cui-rong★, Studying for master’s degree, Physician, Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China zhuozhi1014@163.com

PDF

344

被引次数

9

摘要：

背景：既往研究表明，人参皂苷既能促进正常血细胞生成，又能抑制白血病细胞的增殖，人参皂苷Re是人参皂苷的一类主要有效成分，有多种活性，但是对白血病细胞的作用及机制尚不清楚。
目的：观察人参皂苷Re对人慢性粒细胞白血病细胞株(K562细胞)分化的影响。
方法：取对数生长期的K562细胞，调整细胞浓度为7×108 L-1。空白对照组予以RPMI1640 培养基常规培养；人参皂苷Re组予以含人参皂苷Re的RPMI1640 培养基培养，终浓度分别为30，60，90，120，150 μmol/L。Wright’s染色、联苯胺染色、血红蛋白测定检测K562细胞向红系细胞分化的特征，流式细胞术测定细胞表面标志物表达。
结果与结论：人参皂苷Re 90 μmol/L可以诱导K562细胞向早期红系细胞分化，表现为：①K562 细胞形态学上可见细胞体积缩小，核直径减小，胞浆丰富，核浆比例降低。②人参皂苷Re在体外对K562细胞有诱导血红蛋白生成的作用。③细胞膜表面CD13阳性率增加。

关键词: 人参皂苷Re, K562细胞, 诱导分化, 联苯胺染色, 白血病

Abstract:

BACKGROUND: Gensingnosides can promote normal hematopoiesis and inhibit proliferation of leukemia cells. Gensingnoside Re as the main active ingredient in Ginsenosides has a variety of activities. However, Re induces leukemia cells differentiation and the mechanisms remain unclear.
OBJECTIVE: To investigate the effect of Gensingnoside Re on differentiation of chronic myelocytic leukemia K562 cells.
METHODS: The density of K562 cells at logarithmic phase was regulated to 7×108/L. Cells in the blank control group were routinely cultured in RPMI1640 medium. Cells in the Gensingnoside Re group were incubated in RPMI1640 medium containing Gensingnoside Re with the final concentration of 30, 60, 90, 120, 150 μmol/L. Wright’s staining, benzidine staining and hemoglobinometry were utilized to determine the characteristics of K562 cell differentiation into erythroid cells. Expressions of markers on the surface of K562 cells were determined by flow cytometry. 
RESULTS AND CONCLUSION: Gensingnoside Re 90 μmol/L could induce the K562 cell differentiation into erythroid cells. ① The morphological feature of K562 cells presented diminished volume, reduced nuclear diameter, abundant cytoplasm, and decreased karyoplasmic ratio. ②Hemoglobin in K562 cells induced by gensingnoside Re increased significantly in vitro. ③CD13 expressions on the surface of K562 cells increased.

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