中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9221-.doi: 10.3969/j.issn.1673-8225.2010.49.023

• 干细胞与中医药 • 上一篇    下一篇

黄精含药血清促进骨髓间充质干细胞增殖的效应及机制

黄进，张进，徐志伟   

  1. 广州中医药大学，广东省广州市 510006
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: Zhang Jin, Doctor, Associate professor, Fundamental Medical Science College, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China zhjin@gzhtcm.edu.cn
  • 作者简介:黄进☆，女，1981年生，湖南省衡阳市人，汉族，广州中医药大学毕业，博士，主要从事肾藏精的基础与应用研究。 kitty_jinjin@163.com
  • 基金资助:

    国家自然科学基金项目：电针内关穴对骨髓间充质干细胞移植治疗心肌梗塞的影响研究(30500645)。

Promoting effect and mechanism of polygonatum medicated serum on bone marrow mesenchymal stem cells proliferation 

Huang Jin, Zhang Jin, Xu Zhi-wei   

  1. Fundamental Medical Science College, Guangzhou University of Chinese Medicine, Guangzhou  510006, Guangdong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: 张进，博士，副教授，广州中医药大学解剖教研室，广东省广州市 510006 zhjin@gzhtcm.edu.cn
  • About author:Huang Jin☆, Doctor, Fundamental Medical Science College, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China kitty_jinjin@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30500645*

PDF

456

被引次数

28

摘要：

背景：近年来，对传统单味中药有效成分、复方中药及含药血清调控骨髓间充质干细胞增殖与分化作用的研究已有报道，但鲜有涉及到机制的研究。
目的：观察黄精含药血清体外对骨髓间充质干细胞增殖的影响，并分析其作用途径与机制。
方法：雄性SD大鼠用于骨髓间充质干细胞的提取；雌性SD大鼠以中药水溶液灌胃后定时取血制备含药血清。全骨髓贴壁法分离培养大鼠骨髓间充质干细胞，将传至P3代的细胞，制备成单细胞悬液接种于96孔培养板后，分别加入黄精含药血清及空白血清，各组血清浓度分别为培养液体积的5%，10%，15%，20%，25%及30%。MTT法检测黄精含药血清对骨髓间充质干细胞增殖的影响；RT-PCR检测各细胞因子mRNA表达；实时PCR检测粒细胞集落刺激因子mRNA表达。
结果与结论：与空白血清组相比，体积分数10%的黄精含药血清具有明显促进骨髓间充质干细胞增殖的作用(P < 0.05)，并显著促进粒细胞集落刺激因子mRNA的表达(P < 0.05)。提示体积分数10%的黄精含药血清具有促进骨髓间充质干细胞增殖的作用，可能与其促进粒细胞集落刺激因子mRNA表达有关。

关键词: 黄精, 含药血清, 骨髓间充质干细胞, 增殖, 粒细胞集落刺激因子

Abstract:

BACKGROUND: Current studies focus on the observation of single or complex Chinese medicine and serum containing drug on bone marrow mesenchymal stem cells (BMSCs) proliferation and differentiation, but few reports have addressed the mechanisms.
OBJECTIVE: To investigate the effect of polygonatum medicated serum on BMSCs proliferation in vitro, and to analyze its action pathway and mechanism.
METHODS: Male Sprague-Dawley rats were used for the isolation of BMSCs cells; the serum of female Sprague-Dawley rats was obtained after intragastric administration with medicine water solution. Rat BMSCs were isolated and purified by whole bone marrow adherent method, purified by trypsin. The third passage of BMSCs were prepared into single cell suspension, and incubated in the 96-well culture plate. The experimental group was added polygonatum medicated serum, while the control group added blank serum at concentration of 5%, 10%, 15%, 20%, 25%, 30%. The polygonatum medicated serum-induced proliferation effect on BMSCs was detected by MTT assay. Different cytokine mRNA expression was measured by RT-PCR. Granulocyte colony-stimulating factor mRNA expression was analyzed by real-time PCR.
RESULTS AND CONCLUSION: Compare with the blank serum group, 10% polygonatum medicated serum significantly promoted the proliferation of BMSCs (P < 0.05), and significantly improved the expression of granulocyte colony-stimulating factor mRNA and protein (P < 0.05). Results suggest that 10% polygonatum medicated serum can promote proliferation of BMSCs, and the mechanism involves the stimulation of granulocyte colony-stimulating factor mRNA expression.

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