中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (47): 8773-8776.doi: 10.3969/j.issn.1673-8225.2010.47.008

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

丝素蛋白/磷酸钙骨水泥体外强化骨缺损椎体

陈晓庆1，赵  剑1，张  烽1，王根林2，干旻峰2，顾  勇2，朱雪松2，杨惠林2   

  1. 1南通大学附属医院骨科，江苏省南通市  226001；2苏州大学附属第一医院骨科，江苏省苏州市  215006
  • 出版日期:2010-11-19 发布日期:2010-11-19
  • 通讯作者: 杨惠林，教授，主任医师，苏州大学附属第一医院骨科，江苏省苏州市 215006 osteogen@gmail.com
  • 作者简介:陈晓庆☆，男，1981年生，江苏省南通市人，汉族，苏州大学毕业，博士，医师，主要从事脊柱外科、创伤骨科、人工骨研究。 Chenxiaoqing 1981@gmail.com

Vertebral body defect augmentation with silk fibroin/calcium phosphate cement in vitro

Chen Xiao-qing1, Zhao Jian1, Zhang Feng1, Wang Gen-lin2, Gan Min-feng2, Gu Yong2, Zhu Xue-song2, Yang Hui-lin2   

  1. 1 Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong   226001, Jiangsu Province, China; 2 Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou   215006, Jiangsu Province, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Yang Hui-lin, Professor, Chief physician, Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China osteogen@gmail.com
  • About author:Chen Xiao-qing☆, Doctor, Physician, Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China chenxiaoqing1981@gmail.com

PDF

412

被引次数

3

摘要：

背景：目前常用的椎体强化剂筛选方法是将尸体标本制成骨折模型，然后进行材料的灌注，这种模型很难进一步应用于体内实验。
目的：制备一种椎体骨缺损模型，评价骨水泥增强后的力学性能。
方法：将48个新鲜成年绵羊腰椎单椎体标本随机分为8组。取4组标本，利用直径分别为2.0，4.0，6.0，8.0 mm的钻头钻入椎体10.0 mm制备骨缺损椎体模型，测量单椎体的抗压强度和刚度，与正常椎体组进行比较。根据生物力学测试结果选择一固定直径，将剩下的3组制成骨缺损模型，然后分别注入磷酸钙骨水泥、丝素蛋白/磷酸钙骨水泥及聚甲基丙烯酸甲酯骨水泥，模拟体液环境固化24 h后进行生物力学测试。
结果与结论：不同直径的椎体骨缺损模型中，随着缺损直径增大，其抗压强度与刚度呈逐渐下降趋势，直径为6.0 mm下降明显。选择直径为6.0 mm钻头制备骨缺损椎体模型，丝素蛋白/磷酸钙骨水泥组及聚甲基丙烯酸甲酯骨水泥组的抗压强度和刚度与正常椎体组相比无明显差别(P > 0.05)，而磷酸钙骨水泥组的抗压强度及刚度均明显低于正常椎体组(P < 0.05)。说明缺损直径为6 mm，深度为10 mm绵羊椎体骨缺损是一种合适的椎体骨缺损模型，保持了椎体外形的完整，可用来判断椎体强化剂的体外性能，进一步用于体内实验。丝素蛋白/磷酸钙骨水泥能有效即时强化骨缺损椎体。

关键词: 丝素蛋白/磷酸钙骨水泥, 骨缺损, 椎体, 体外, 绵羊

Abstract:

BACKGROUND: Injection into the vertebral fracture model of corpse is the most common used method for screening bone cement biomechanically in vertebral augmentation. This model can hardly be applied in the further studies in vivo.
OBJECTIVE: To develop a vertebral body defect model for screening vertebral augmentation filler materials and to evaluate the mechanical property after the bone cement augmentation.
METHODS: Forty-eight lumbar vertebrae harvested from ten fresh skeletally mature female sheep cadavers were evenly assigned to eight groups, 6 vertebrae in each. Four groups of vertebrae were drilled in the perpendicular direction to center of the vertebra, with the different drills at a diameter of 2.0, 4.0, 6.0, 8.0, to form a cylindrical bone defect. All vertebrae were compressed to determine the strength and stiffness, which were compared with normal vertebrae group. Based on the biomechanical test, a certain defect was selected as the vertebral body defect model. The remaining 3 groups were prepared into bone defect models, and then augmented by calcium phosphate cement, silk fibroin/calcium phosphate cement, polymethyl methacrylate, respectively. The biomechanical test was performed after setting for 24 hours in simulated body fluid.
RESULTS AND CONCLUSION: The compressive strength and stiffness of the vertebrae with bone defect gradually decreased as the diameter of the bone defect increased, and the decline was remarkable in the vertebrae at a diameter of 6.0 mm. After bone defect in vertebrae was produced using a 6.0-mm-diameter drill, there was no difference in the compressive strength and stiffness of the vertebrae among silk fibroin/calcium phosphate cement, polymethyl methacrylate compared with normal vertebrae group (P > 0.05), while calcium phosphate cement group had a lower value compared with normal vertebrae group (P < 0.05). The sheep vertebra with a 6.0-mm diameter and 10.0-mm depth defect is a suitable model to screen vertebral augmentation filler materials. This model keeps the vertebral figuration intact and can be used to adjust the performance of vertebral enhancer in vitro, so it can be used in further studies in vivo. Silk fibroin/calcium phosphate cement has a prompt and efficient augmentation effect in bone defect vertebral model.

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