中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8585-.doi: 10.3969/j.issn.1673-8225.2010.46.009

• 组织构建与生物力学 • 上一篇    下一篇

模拟失重与骨组织的细胞凋亡

麦燕兴1，黄震2，简炼1，秦佳升3，杜江4，邓伟民5   

  1. 南方医科大学珠江医院，1内分泌科，2外科学教研室，3肝胆外科， 4再生医学研究所，广东省广州市 510282；5解放军广州军区广州总医院华侨科，广东省广州市  510282
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 黄震，男，教授，硕士生导师。南方医科大学第二临床学院外科教研室主任，广东省医学会骨质疏松分会荣誉主任。南方医科大学珠江医院外科学教研室，广东省广州市 510252 hzhen2@sina.com
  • 作者简介:麦燕兴★，女，1981年生，广东省中山市人，南方医科大学在读硕士，主要从事内分泌与代谢病方面的研究。 maiyanxing123@163.com

Simulated weightlessness and apoptosis in bone tissue

Mai Yan-xing1, Huang Zhen2, Jian Lian1, Qin Jia-sheng3, Du Jiang4, Deng Wei-min5   

  1. 1Department of Endocrinology, 2Department of Surgery, 3Department of Hepatobiliary Surgery, 4Institute for Regeneration Medical, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China; 5Department of Overseas Chinese, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510282, Guangdong Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Huang Zhen, Professor, Master’s supervisor, Department of Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China hzhen2@sina.com
  • About author:Mai Yan-xing★, Studying for master’s degree, Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China maiyanxing123@163.com

PDF

375

被引次数

8

摘要：

背景：空间骨丢失主要表现为成骨细胞、骨细胞活性减弱，而包括Fas蛋白在内的诸多因素可调节成骨细胞、骨细胞的增殖和分化并最终调控骨形成。
目的：观察失重对骨组织中细胞凋亡的影响。
方法：5周龄SPF级雄性SD大鼠36只，随机分为尾吊模拟失重组及对照组。建立大鼠尾吊模拟失重模型，建模后7，14，21 d，使用自动生化仪检测大鼠血清中钙、磷、碱性磷酸酶含量，放射免疫分析法测定骨钙素含量，原位细胞凋亡检测技术检测骨组织中细胞凋亡，免疫组化方法检测骨组织中Fas蛋白含量。
结果与结论：尾吊模拟失重组大鼠血清中碱性磷酸酶、骨钙素含量均显著低于对照组(P < 0.05)，但血钙、磷浓度均较相应对照组升高，骨组织中成骨细胞、骨细胞及骨髓间充质细胞凋亡指数、骨组织中Fas抗原含量均高于相应对照组(P < 0.01)。说明尾部悬吊模拟失重增加骨组织中Fas蛋白表达，从而增加大鼠骨组织中成骨细胞、骨细胞及骨髓间充质细胞凋亡。

关键词: 尾吊, 模拟失重, 生化, 骨钙素, 细胞凋亡, Fas蛋白

Abstract:

BACKGROUND: Bone loss in space presents with weakened activity of osteoblasts and osteocytes. Many factors, including Fas protein, can regulate the proliferation and differentiation of osteoblasts and osteocytes, ultimately, regulate bone formation.
OBJECTIVE: To observe the effects of weightlessness on apoptosis of bone tissues.
METHODS: Thirty-six male Sprague Dawley rats were randomly assigned to simulated weightlessness and control groups. The rats were suspended by the tail for simulating weightlessness. At 7, 14 and 21 days after simulated weightlessness, contents of serum calcium, phosphorus, and alkaline phosphatase were observed by an automated analyzer. The level of bone gla protein was measured by radio immunoassay. Condition of cell apoptosis in bone tissues was detected by in situ death detection. The expressions of Fas protein were detected with SP immunohistochemical method.
RESULTS AND CONCLUSION: The contents of serum bone gla protein and alkaline phosphatase were significantly lower in the simulated weightlessness group than those in corresponding control group (P < 0.05), whereas serum calcium, phosphorus concentration were increased, and the apoptotic index of osteoblasts, osteocytes and bone marrow stromal cells were higher than that in corresponding control group (P < 0.01). Skeletal unloading in rats induces an increase in Fas protein expression, thus, increase the apoptosis of osteoblasts, osteocytes and bone marrow cells.

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