中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8175-8178.doi: 10.3969/j.issn.1673-8225.2010.44.002

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

低温保存同种异体带瓣主动脉移植的免疫反应

周  政，李东玉   

  1. 中国医科大学附属盛京医院心脏外科，辽宁省沈阳市  110004
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 李东玉，博士，教授，中国医科大学附属盛京医院心脏外科，辽宁省沈阳市 110004
  • 作者简介:周政★，男，1983年生，辽宁省沈阳市人，汉族，硕士，医师，主要从事异体移植的免疫反应排斥研究。 mysky007411@hotmail.com

Immunological reaction of allogenic aortic valve transplantation following hypothermic preservation

Zhou Zheng, Li Dong-yu   

  1. Department of Cardiac Surgery, Shengjing Hospital, China Medical University, Shenyang  110004, Liaoning Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Li Dong-yu, Doctor, Professor, Department of Cardiac Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
  • About author:Zhou Zheng★, Master, Physician, Department of Cardiac Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China mysky007411@hotmail.com

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摘要：

背景：前期研究证实，液氮低温保存同种异体主动脉能保持其细胞的生物活性和组织结构的完整性，但有关同种异体带瓣主动脉移植的排斥反应研究较少。
目的：观察同种异体带瓣主动脉移植后移植物的免疫指标变化特点。
方法：将低温保存的SD大鼠带瓣主动脉异位植入Wistar大鼠腹主动脉，分组干预：同种异体移植组不进行干预；免疫干预组移植后给予环孢素A；对照组为假移植，将腹主动脉切断后直接吻合。移植后1，2，3，4，5周收集移植动物血清标本，免疫组织化学染色测量MHCⅡ阳性表达，淋巴细胞涂片免疫组织化学测定CD4+ 、CD8+百分率；应用酶联法测定血清中白细胞介素2，肿瘤坏死因子α水平。
结果与结论：同种异体主动脉移植后1~4周可见MHC显著阳性，CD4+ 、CD4+ /CD8+比值明显增高，血清中白细胞介素2和肿瘤坏死因子α水平明显增高，并在移植后5周内维持较高水平，与对照组比较差异有显著性意义(P < 0.05)。环孢素A对同种异体主动脉移植物内膜增厚、淋巴细胞浸润、MHCⅡ表达、T淋巴细胞激活、白细胞介素2和肿瘤坏死因子α水平的增高具有明显抑制作用(P < 0.05)。说明低温保存同种异体主动脉移植后有明确的免疫排斥反应发生，环孢素A对同种异体移植后的免疫排斥反应有明显抑制作用。

关键词: 主动脉, 同种异体, 移植, 免疫排斥, 器官移植

Abstract:

BACKGROUND: Previous studies demonstrated that nitrogen cryopreservation can maintain cell biological activity and tissue construction integrality, however, there are few studies concerning immunological reaction of allogenic aortic valve transplantation.
OBJECTIVE: To observe the immune parameters changes following allogenic aortic valve transplantation.
METHODS: Hypothermic preserved allogenic aortic valve was transplanted into Wistar rats, and divided into groups. There was no intervention on the allogenic transplantation group; Ciclosporin A was injected into rats in the immune prevention group; in the control group, the aorta was cut down and re-anastomosed directly. The serum specimen was collected at 1, 2, 3, 4 and 5 weeks after transplantation. The MHCⅡ positive expression and percentage of CD4+ , CD8+ cells were measured by immunohistochemistry. The serum interleukin 2 and tumor necrosis factor α levels were measured by ELISA.   
RESULTS AND CONCLUSION: MHC positive could be seen at 1-4 weeks after transplantation, and the ratio of CD4+ , CD4+ /CD8+ was obviously increased, the serum interleukin 2 and tumor necrosis factor α levels were dramatically increased, and maintained a high level within 5 weeks after transplantation. Compared with the control group, there was significant difference (P < 0.05). Ciclosporin A exhibited an obvious inhibitory effect on intimal thickening, lymphocyte infiltration, MHCⅡ expression, T lymphocyte activation, as well as increased interleukin 2 and tumor necrosis factor α levels after allogenic transplantation (P < 0.05). The findings demonstrated that, there are obvious immunological rejections of allogenic aortic valve transplantation following hypothermic preservation, but ciclosporin A has notably inhibitory action.

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