中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7704-7708.doi: 10.3969/j.issn.1673-8225.2010.41.025

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

短期局部注射激素大鼠肌腱的生物力学和病理学变化

姬树青1，2，马信龙1，3，马剑雄1，朱少文3，杨  阳1   

  1. 1天津医科大学总医院骨科，天津市 300052；2天津市蓟县人民医院骨科，天津市 301900；3天津医院，天津市 300211
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 马信龙，硕士，教授，天津医科大学总医院骨科，天津市 300052；天津医院，天津市 300211 mjx969@163.com
  • 作者简介:姬树青★，男，1979年生，天津市蓟县人，汉族，天津医科大学在读硕士，主要从事骨科生物力学方面的研究。 jshq0_wmh6818@sina.com.cn
  • 基金资助:

    天津市自然科学基金资助项目(043111411)，课题名称“个体化人工股骨头优化设计和应用的实验研究”；天津市自然科学基金资助项目(993607711)，课题名称“废用性骨质疏松形成过程中骨改建活动的研究”。

Biomechanical and pathological changes of rat tendon following short-term local glucocorticosteroid injection

Ji Shu-qing 1,2 , Ma Xin-long 1,3, Ma Jian-xiong1, Zhu Shao-wen3, Yang Yang1   

  1. 1 Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin  300052, China; 2 Department of Orthopedics, the People’s Hospital of Jixian County, Tianjin  301900, China; 3Tianjin Hospital, Tianjin  300211, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Ma Xin-long, Master, Professor, Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin 300052, China; Tianjin Hospital, Tianjin 300211, China
  • About author:Ji Shu-qing★, Studying for master’s degree, Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin 300052, China; Department of Orthopedics, the People’s Hospital of Jixian County, Tianjin 301900, China jshq0_wmh6818@sina.com.cn
  • Supported by:

     the Natural Science Foundation of Tianjin, No. 043111411*; 993607711*

PDF

395

被引次数

7

摘要：

背景：研究证实激素长期局部注射对肌腱是有负面影响的，但短期应用激素对肌腱的影响仍存在争议。
目的：观察短期激素局部注射后大鼠肌腱生物力学和病理方面的变化。
方法：激素封闭对照组、激素封闭训练组大鼠均按人体局部封闭疗程进行治疗，于双侧跟腱的腱周部位注射与人体相当剂量地塞米松0.025 mg，1次/周，3次为1个疗程方法。激素封闭训练组保证跑步训练，跑步强度为25 m/min，30 min/次，3次/周。正常对照组大鼠自由活动，无任何处理。分别于第4，7周取肌腱行苏木精-伊红染色和MASSON染色，观察炎细胞浸润及胶原纤维排列情况，并进行跟腱拉伸实验以观察其生物力学变化。
结果与结论：激素封闭对照组力学性能呈明显先增强后下降的趋势，并出现1例肌腱断裂实例，而激素封闭训练组力学性能呈明显先下降后恢复的趋势，两组病理均表现为肌腱退变。提示短期激素局部注射对肌腱是一种刺激，可使肌腱的适应性下降，应用时需避免强体力劳动，可进行适度功能锻炼。

关键词: 肌腱, 生物力学, 病理, 封闭, 激素, 组织构建

Abstract:

BACKGROUND: The long-term local injection of glucocortico steroid have a negative impact on the tendon, but the short-term application is still in dispute. 
OBJECTIVE: To observe the biomechanical and pathological changes of rat tendon following short-term glucocorticosteroid local injection.     
METHODS: Rats in the hormone blockade control and hormone blockade training groups were injected dexamethasone with human body suitable dose once weekly and three times totally. Treadmill exercise was supplemented to rats in the hormone blockade training group, with intensity of 25 m per minutes, 30 minutes per time, and three times weekly. There was no treatment on rats in the normal control group. Hematoxylin-eosin staining and MASSON staining were used to observe tendon histological alterations. Tension test was performed to observe the biomechanical changes.
RESULTS AND CONCLUSION: Biomechanical features of the hormone blockade control group appeared earlier reinforced and lately degraded, and a case of tendon rupture was found. The mechanics efficiency of tendon in the hormone blockade training group showed earlier degraded and lately recovered. Tendon cataplasia could be found in these two groups. Short-term local glucocorticosteroid injection can stimulate and decline the flexibility of the tendon. Strong physical strength work should be avoided and moderate functional exercise should be done when use it.

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