中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7709-7712.doi: 10.3969/j.issn.1673-8225.2010.41.026

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

家兔胫前肌肌亚体诱发电位和梭内外肌纤维出生后的增龄改变

朱道立1，王康乐1，徐大刚2   

  1. 1南通大学生命科学学院，江苏省南通市  226007；  2上海交通大学医学院，上海市  200025
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 徐大刚，硕士，副教授，上海交通大学医学院，上海市 200025 jsc＠shsmu.edu.cn
  • 作者简介:朱道立，男，1951年生，上海市人，汉族，1977年遵义医学院毕业，教授，主要从事动物学研究。 zhudaoli＠ntu.edu.cn
  • 基金资助:

    南通市科技局社会发展科技计划资助项目(S2008022)，项目名称：骨骼肌卫星细胞在神经肌肉系统疾病治疗的应用研究。

Changes of postnatal ages for the evoked potential and extrafusal, intrafusal fibers of muscle subvolume of tibial muscle in domestic rabbits

Zhu Dao-li1, Wang Kang-lei1, Xu Da-gang2   

  1. 1 Life Science College of Nantong University, Nantong  226007, Jiangsu Province, China; 2 Shanghai Jiao Tong University School of Medicine, Shanghai  200025, China　
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Xu Da-gang, Master, Associate professor, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China jsc＠shsmu.edu.cn
  • About author:Zhu Dao-li, Professor, Life Science College of Nantong University, Nantong 226007, Jiangsu Province, China zhudaoli＠ntu.edu.cn
  • Supported by:

    the Science and Technology Planned Project for Social Development from Science and Technology Bureau of Nantong City, No. S2008022*

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摘要：

背景：家兔骨骼肌生后发育过程中，梭内外肌纤维酶组织化学构成比例和肌电特征的变化反映其与功能的关系。目前，国内外缺乏对长肌胫前肌肌亚体的相关结构功能的系统研究。
目的：探讨生后不同年龄阶段家兔胫前肌肌亚体的诱发电位和酶组织化学表现。
方法：应用大体解剖结合酶组织化学法明确不同年龄阶段家兔胫前肌肌亚体。来用电生理记录仪测量家兔胫前肌肌亚体的诱发电位振幅、持续时间及波形，ATP酶组织化学染色观察ⅡB型、ⅡA型、ⅡX型、Ⅰ型梭外肌纤维组成，梭内肌纤维的构成特点。
结果与结论：幼年家兔的诱发电位振幅、持续时间及波形波动均较小，波形相对较简单且波动平缓；随着年龄增长，成年后诱发电位振幅、持续时间及波形波动较大，波形比幼年的延续时间长而复杂，有小的棘波出现。胫前肌前、后亚体内ATP酶组织化学染色显示ⅡB型、ⅡA型梭外肌纤维随增龄而增加；而ⅡX型、Ⅰ型梭外肌纤维随增龄而减少。梭内肌纤维分为核袋纤维b1、b2和核链纤维c，其外被结缔组织构成的梭囊包绕。结果表明在行走和奔跑状态下，后亚体协助维持小腿的姿势和保持后肢关节的稳定性，当运动需要力量和速度时，前亚体与后亚体一起提供足够的收缩速度和力量以适应运动的需要。

关键词: 胫前肌, 诱发电位, 腺苷三磷酸酶, 梭内肌纤维, 梭外肌纤维, 家兔

Abstract:

BACKGROUND: Electromyography characteristic and constitution percentage of enzyme histochemistry of intrafusal fibers and extrafusal fibers were interrelated with function during postnatal development of rabbit muscle. However, systematic studies addressing muscle subvolume of tibial muscle are insufficient.
OBJECTIVE: To inquire into the evoked potential and enzyme histochemistry of tibial muscle subvolume in dometic rabbits with different ages.
METHODS: General dissection combined with enzyme histochemistry was applied to explicit tibial muscle subvolume in dometic rabbits with different ages; at the same time, the function of muscle subvolume was surveyed using electric physiology record instrument. The inducing potential amplitude, continued time and wave motion, extrafusal ⅡB, ⅡA, ⅡX, Ⅰ fiber composition were stained by ATPase histochemistry and the feature of intrafusal fibers were observed.
RESULTS AND CONCLUSION: The inducing potential amplitude, continued time and wave motion were smaller, the spectrum was simple and wave motion was gentle at childhood. With age increased, the inducing potential amplitude, continued time and wave motion at adult were bigger, and the spectrum was longer and complicated than the continued time of childhood. Small spinal wave appeared. Extrafusal ⅡB, ⅡA fiber of stained ATPase at anterior and posterior subvolume of tibial muscle were increased with age increasing. But the extrafusal ⅡX, Ⅰ fiber of stained ATPase at anterior and posterior subvolume of tibial muscle were decreased from postnatal ages. Intrafusal fibers were divided into nuclear bag1, nuclear bag2 and nuclear chain fibers. They were wrapped with intracapsular and extracapsular regions composed of connective tissues. Posterior subvolume maintains hind legs’ pose and joint of posterior limb with stability during standing and walking. Anterior and posterior subvolume were supplied together sufficient contractive speed and power for need of movement when the motion were needed strength and velocity.

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