中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (7): 1196-1200.doi: 10.3969/j.issn.2095-4344.2013.07.011

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

慢性颈脊髓压迫模型大鼠构建及评价

王 军1，韦 峰1，汪辉亮2，赵 靖2，容 威1，胡 星1，刘忠军1   

  1. 1 北京大学第三医院骨科，北京市 100191
    2北京师范大学化学学院，北京市 100875
  • 收稿日期:2012-06-13 修回日期:2012-07-13 出版日期:2013-02-12 发布日期:2013-02-12
  • 通讯作者: 刘忠军，硕士，教授，主任医师，北京大学第三医院骨科，北京市 100191 zjliu@bjmu.edu.cn
  • 作者简介:王军★，男，1986年生，山西省大同市人，汉族，北京大学第三医院在读硕士，主要从事脊柱脊髓损伤的相关研究。 pkuwj2010@sina.cn

Establishment and evaluation of a rat model of chronic cervical spinal cord compression

Wang Jun1, Wei Feng1, Wang Hui-liang2, Zhao Jing2, Rong Wei1, Hu Xing1, Liu Zhong-jun1   

  1. 1 Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China
    2 College of Chemistry, Beijing Normal University, Beijing 100875, China
  • Received:2012-06-13 Revised:2012-07-13 Online:2013-02-12 Published:2013-02-12
  • Contact: Liu Zhong-jun, Master, Professor, Chief physician, Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China zjliu@bjmu.edu.cn
  • About author:Wang Jun★, Studying for master’s degree, Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China pkuwj2010@sina.cn

PDF

732

被引次数

7

摘要：

背景：在脊髓型颈椎病脊髓损伤发生机制的研究过程中，建立稳定且同人类体内疾病演变过程相似的疾病模型对于研究脊髓型颈椎病发病机制至关重要。
目的：构建慢性颈脊髓压迫模型，观察该模型病理生理变化特点，进一步明确脊髓型颈椎病受压脊髓组织的病理改变。
方法：30只SD大鼠随机均分为对照组、轻度压迫组、重度压迫组。将不同大小吸水性压迫材料聚乙烯醇丙烯酰胺互穿网络水凝胶植入C5-C7椎板下，制作慢性颈脊髓压迫动物模型，对照组不植入压迫材料。
结果与结论：MRI检查显示两压迫组大鼠出现不同程度的椎管狭窄和脊髓压迫，而对照组椎管宽度正常无脊髓压迫。电生理检测显示两压迫组大鼠运动诱发电位潜伏期较对照组明显延长且振幅明显降低(P < 0.05)。神经元免疫荧光染色显示对照组大鼠脊髓有大量形态规则的神经元，而两压迫组大鼠神经元计数明显减少且神经元胞体形态明显皱缩，脱髓鞘现象明显，3组间比较差异有显著性意义(P < 0.05)。压迫组大鼠脊髓压迫节段发现较多凋亡细胞，而对照组未发现。说明构建的大鼠慢性颈脊髓压迫模型符合脊髓型颈椎病的病理改变，且手术操作简便，不易感染死亡率低；神经元损伤、脱髓鞘改变和凋亡机制参与了大鼠慢性颈脊髓压迫损伤的发生发展过程。

关键词: 组织构建, 组织构建实验造模, 慢性脊髓压迫, 动物模型, 大鼠, 组织学, 运动诱发电位, 脱髓鞘, 脊髓型颈椎病, 组织构建细胞图片, 国家自然科学基金

Abstract:

BACKGROUND: Establishing a stable model of cervical spondylotic myelopathy is essential for the study of cervical spondylotic myelopathy pathogenesis.
OBJECTIVE: To clarify the mechanism of cervical spondylotic myelopathy by establishing a cervical spondylotic myelopathy model and observing the pathophysical changes of this model.
METHODS: A total of 30 Sprague-Dawley rats were divided into three groups: control group, mild compression group and severe compression group. Water-absorbing compression materials of different size were implanted below the C5-7 to prepare chronic cervical spinal cord compression models. Control group was implanted nothing.
RESULTS AND CONCLUSION: The normal morphology in the control group and varying degrees of compression on the spinal cord and spinal canal stenosis in the two compression groups were shown in MRI. The lower amplitude and longer latency of motor evoked potential after spinal cord compression was found in the two compression groups as compared with the control group (P < 0.05). Immunofluorescence staining showed that that a great number of healthy-shaped neurons were found in the control group. But, the number of neurons in the two compression groups was significantly decreased, and the neurons shrunk and demyelination was obvious. The difference of neuron number among the three groups was statistically significant (P < 0.05). Apoptosis in the segment of compressed spinal cord was found in the two compression groups rather than in the control group. In conclusion, rat models of chronic spinal cord compression are in line with the pathological changes of cervical spondylotic myelopathy. The surgical procedure is simple and the rate of infection and mortality is lower. Neuronal injury, demyelination and apoptosis are involved in the process of chronic spinal cord compression in rats.

Key words: tissue construction, experimental modeling in tissue construction, chronic spinal cord compression, animal models, rats, histology, motor evoked potentials, demyelination, cervical spondylotic myelopathy, tissue construction photographs-containing paper, the National Natural Science Foundation of China

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