中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7621-7625.doi: 10.3969/j.issn.1673-8225.2010.41.006

• 骨组织构建 bone tissue construction • 上一篇    下一篇

人成骨细胞增殖和分化与生长激素释放肽

廖乐乐，王万春   

  1. 中南大学湘雅二医院骨科，湖南省长沙市  410011
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 王万春，博士，教授，中南大学湘雅二医院骨科，湖南省长沙市 410011 wwc1962@yahoo.com.cn
  • 作者简介:廖乐乐☆，1982年生，湖南省衡阳市人，汉族，2010年中南大学湘雅医学院毕业，博士，医师，主要从事关节外科、骨性关节炎的发病机制和治疗的研究。 L820207@163.com

Effects of ghrelin on the proliferation and differentiation of human osteoblasts

Liao Le-le, Wang Wan-chun   

  1. Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha  410011, Hunan Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Wang Wan-chun, Doctor, Professor, Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China wwc1962@yahoo.com.cn
  • About author:Liao Le-le☆, Doctor, Physician, Department of Orthopaedics, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China L820207@163.com

PDF

425

被引次数

4

摘要：

背景：有研究表明生长激素释放肽对体外培养大鼠成骨细胞增殖有促进作用，但对人成骨细胞增殖和分化是否有影响却少有报道。
目的：探讨生长激素释放肽对人成骨细胞增殖和分化的影响。
方法：取正常成人髂前上棘松质骨，分离出成骨细胞并进行体外培养，应用RT-PCR法检测生长激素促分泌素受体的表达；3H-掺入法检测成骨细胞增殖；Westernblot检测与成骨细胞分化相关的Runx2蛋白表达；α-磷酸奈酚法和放射免疫法观察生长激素释放肽对人成骨细胞分化的影响。
结果与结论：人成骨细胞可表达生长激素促分泌素受体mRNA和生长激素促分泌素受体蛋白。生长激素释放肽促进人成骨细胞增殖(P < 0.05或P < 0.01)，且呈剂量依赖性，而且可增加碱性磷酸酶的活性并促进骨钙素的分泌(P < 0.05或P < 0.01)，但对人成骨细胞中Runx2蛋白的表达无影响。结果提示，生长激素释放肽能促进人成骨细胞增殖和分化。

关键词: 生长激素释放肽, 人成骨细胞, 细胞增殖, 细胞分化, 组织构建

Abstract:

BACKGROUND: Ghrelin has been reported to have a role in the regulation of bone metabolism on rats, but whether it has the effects on the proliferation and differentiation of human osteoblasts remains unclear.
OBJECTIVE: To investigate the effects of ghrelin on the proliferation and differentiation of human osteoblasts.
METHODS: Human osteoblasts were separated from normal human cancellous bone in anterior superior spine, and cultured in vitro. The expression of GHS-R1a in human osteoblasts was verified by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Human osteoblasts proliferation was assessed by measuring [3H] thymidine (2Ci/ml) incorporation into trichloroacetic acid (TCA)-insoluble material. The differentiations of human osteoblasts were evaluated by RIA and ELISA. The expression of Runx2 in human osteoblasts was detected by Western blot.
RESULTS AND CONCLUTION: The mRNA and protein of GHS-R1a were expressed in human osteoblasts. Ghrelin promoted the proliferation of human osteoblasts in a dose-dependent manner (P < 0.01). Ghrelin increased the activity of alkaline phosphatase (P < 0.01) and the secretion of osteocalcin (P < 0.01) in human osteoblasts. But the protein expression levels of Runx2 in human osteoblasts were no significantly changed by the intervention of ghrelin. The findings demonstrated that ghrelin can efficiently promote the proliferation and differentiation of human osteoblasts.

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