中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7543-7546.doi: 10.3969/j.issn.1673-8225.2010.40.030

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

诱导性多潜能干细胞的应用前景

刘  康1，易强英1，宋桂芹2，冯  刚1   

  1. 1川北医学院附属第二临床医学院组织工程与干细胞研究所，四川省南充市 637000；2川北医学院医学生物学教研室，四川省南充市  637007
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 通讯作者: 冯刚，博士，教授，硕士生导师，川北医学院附属第二临床医学院组织工程与干细胞研究所，四川省南充市637000 lssmd18@gmail.com
  • 作者简介:刘康★，男，1983年生，山东省泰安市人，汉族，2009年四川大学毕业，硕士，研究实习员，主要从事干细胞方向的研究。 kangliu2009@gmail.com
  • 基金资助:

    国家自然科学基金面上项目(30872614)；四川省科技支撑计划项目(2008SZ0103)；四川省杰出青年学科带头人资助计划(2009-05-396)。

Application prospect of induced pluripotent stem cells

Liu Kang1, Yi Qiang-ying1, Song Gui-qin2, Feng Gang1   

  1. 1 Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong  637000, Sichuan Province, China; 2 Department of Medical Biology, North Sichuan Medical College, Nanchong  637007, Sichuan Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Feng Gang, Doctor, Professor, Master’s supervisor, Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong 637000, Sichuan Province, China lssmd18@gmail.com
  • About author:Liu Kang★, Master, Research Assistant, Research Institute of Tissue Engineering and Stem Cells, Second Clinical Hospital Affiliated to North Sichuan Medical College, Nanchong 637000, Sichuan Province, China kangliu2009@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872614*; the Science and Technology Support Program of Sichuan Province, No. 2008SZ0103*; the Grant for Outstanding Youth Leader of Sichuan Province, No. 2009-05-396*

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被引次数

2

摘要：

背景：通过基因转染的方式将已分化的体细胞重编程为诱导性多潜能干细胞，是近年来干细胞领域一项令人瞩目的新技术。由于诱导性多潜能干细胞摆脱了材料来源和伦理学的限制，因此其出现为特异的细胞治疗，特别是再生医学带来新的曙光。
目的：从诱导性多潜能干细胞的制备流程、产生的限制条件与机制、患者诱导性多潜能干细胞的获得及应用前景等方面做一评述。
方法：由第一作者检索PubMed数据库(www.ncbi.nlm.nih.gov/PubMed)2006-01/2010-03有关诱导性多潜能干细胞制备、产生机制的文章，检索词“induced pluripotent stem cells”，限定语言种类为English；同时手工检索部分文章。排除重复性研究，最终纳入34篇符合标准的文献。
结果与结论：诱导性多潜能干细胞系的建立主要包括以下几个步骤：①重组因子的选择。②目的细胞的选择。③重组因子的导入。④重组因子在目的细胞内的表达。⑤诱导性多潜能干细胞的产生。⑥重组细胞的鉴定。DNA甲基化、组蛋白的修饰作用和甲基化以及p53基因的表达在体细胞重编程为多潜能干细胞的过程中具有重要作用。虽然诱导性多潜能干细胞技术的研究仍然处于初级阶段，但是毫无疑问其具有广阔的应用前景。特别是患者特异性和疾病特异性诱导性多潜能干细胞的获得对于更好地理解疾病的发病机制及药物安全性评价提供了巨大的细胞种子资源。

关键词: 诱导性多潜能干细胞, 体细胞, 重编程, 胚胎干细胞, 应用前景

Abstract:

BACKGROUND: Differentiated somatic cells can be reprogrammed into induced pluripotent stem cells (iPS) by transduction of defined transcription factors. Undoubtedly, iPS technology is a great breakthrough of stem cell research in recent years. iPS breaks away from restrictions on source material and ethics. iPS technology elicits a great promise for patient-specific cell therapy and regenerative medicine.
OBJECTIVE: To review iPS preparation procedures, limited conditions and mechanisms, collection and application prospect.
METHODS: The first author retrieved PubMed database (www.ncbi.nlm.nih.gov/PubMed) for literatures concerning iPS preparation and production mechanisms published from January 2006 to March 2010. The key words were “induced pluripotent stem cells” in English. Simultaneously, some articles were retrieved by hand. Duplicated articles were excluded. Finally, 34 literatures were included.
RESULTS AND CONCLUSION: The establishment of iPS mainly contains following procedures: ① choice of recombinant factors; ② choice of target cells; ③ introduction of recombinant factors; ④ expression of recombinant factors in target cells; ⑤ production of iPS; ⑥ identification of recombinant cells. DNA methylation, histone modification and methylation and p53 gene expression play important roles in the process of somatic cells reprogrammed into iPS. The study of iPS technique was still in an early phase, but it has a broad application prospect. In particular, the acquisition of patient-specific and disease-specific iPS provides great cell seed source for understanding the onset mechanism of diseases and for evaluating drug safety.

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