中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7519-7523.doi: 10.3969/j.issn.1673-8225.2010.40.024

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

Thy1.1干细胞对损伤动脉一氧化氮合成酶的影响

刘华东，董少红，姜  昕，罗林杰   

  1. 深圳市人民医院(暨南大学第二临床医学院)心内科，广东省深圳市  518020
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 作者简介:刘华东☆，女，1975年生，山东省即墨市人，汉族，2008年华中科技大学同济医学院毕业，博士，主治医师，主要从事心血管介入的研究。 lhd2578@163.com

Influence of Thy-1.1 stem cell transplantation on nitric oxide synthase of injured artery

Liu Hua-dong, Dong Shao-hong, Jiang Xin, Luo Lin-jie   

  1. Department of Cardiology, Shenzhen People’s Hospital, Second Clinical College, Jinan University, Shenzhen  518020, Guangdong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Liu Hua-dong☆, Doctor, Attending physician, Department of Cardiology, Shenzhen People’s Hospital, Second Clinical College, Jinan University, Shenzhen 518020, Guangdong Province, China lhd2578@163.com

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摘要：

背景：目前干细胞移植对血管成形后再狭窄的影响存在争议。
目的：观察大鼠Thy-1.1干细胞局部移植对大鼠颈总动脉球囊损伤后内膜增生的影响，探讨干细胞移植对内皮型一氧化氮合成酶及诱生型一氧化氮合成酶的影响，并评价干细胞移植对再狭窄的影响与一氧化氮合成酶的关系。
方法：4~6周龄雄性SD大鼠用于制备骨髓Thy-1.1干细胞。雌性SD大鼠随机抽签法法分为3组，干细胞组：于颈总动脉球囊损伤后即刻将约5×106 Thy-1.1干细胞注入至损伤血管局部；损伤组：颈总动脉球囊损伤后局部注入等量生理盐水；对照组：与损伤组共用大鼠，损伤组取材左侧损伤颈总动脉，对照组取右侧未损伤颈总动脉。各组各于术后即刻、3，7，14，21，28 d麻醉并处死大鼠，留取两侧颈总动脉标本。应用组织形态学方法检测内膜增生并进行计算机图像分析，RT-PCR方法检测内皮型一氧化氮合成酶及诱生型一氧化氮合成酶的表达情况。
结果与结论： ①干细胞组内膜面积低于损伤组(P < 0.05)。②损伤组内皮型一氧化氮合成酶mRNA明显低于对照组，诱生型一氧化氮合成酶mRNA表达高于对照组(P < 0.05)。③干细胞组内皮型一氧化氮合成酶mRNA及诱生型一氧化氮合成酶mRNA表达均明显高于损伤组(P < 0.05)。提示Thy-1.1干细胞局部移植可抑制内膜增生，对球囊损伤具有修复作用，这可能与Thy-1.1干细胞增加一氧化氮合成酶的表达，促进大鼠颈总动脉球囊损伤后再内皮化有关。

关键词: 内皮型一氧化氮合成酶, 骨髓干细胞, Thy-1.1抗原, 诱生型一氧化氮合成酶, 血管损伤

Abstract:

BACKGROUND: At present, effects of stem cell transplantation on restenosis following angiopoiesis are controversial. It is of great significance to explore effects of stem cell transplantation on restenosis and its action mechanism.
OBJECTIVE: To study the effect of local transplantation of rat Thy-1.1 stem cells on endothelial hyperplasia following common carotid artery aortic sac, explore effects of stem cell transplantation on endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS), and evaluate the relationship of restenosis and NOS after stem cell transplantation.
METHODS: Male 4-6 weeks Sprague Dawley rats were used for preparing Thy-1.1 stem cells. Female Sprague Dawley rats were randomly divided into three groups. The rats of stem cell group were injected about 5×106Thy-1.1 stem cells into the injured artery after carotid artery injury. The rats from injury group underwent carotid artery injury and were injected with the same amount of saline; the uninjured right carotid arteries of the injury group rats were harvested for control group. The animals were killed immediately and 3, 7, 14, 21, 28 days after injury, and the samples of carotid artery were harvested. The intimal thickness was detected using histomorphological methods, and analyzed using computer image analysis. Reverse transcription-polymerase chain reaction was utilized to determine the expression of eNOS mRNA and iNOS.
RESULTS AND CONCLUSION: The intimal thickness was thinner in stem cell group compared with injury group (P < 0.05). The eNOS mRNA expression was significantly lower and iNOS mRNA was higher in injury group than in control group (P < 0.05). eNOS mRNA and iNOS mRNA expression was significantly higher in stem cell group compared with injury group (P < 0.05). These indicate that local transplantation of Thy-1.1 stem cells decreased intimal formation and could repair saccule injury, which may be associated with the over-expression of NOS and accelerated re-endothelialization following common carotid artery saccule injury.

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