中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6935-6937.doi: 10.3969/j.issn.1673-8225.2010.37.022

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

茶多酚对力竭运动大鼠骨骼肌组织氧化损伤的保护作用

刘  霞   

  1. 上海中医药大学中医营养教研室，上海市  201203
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 作者简介:刘霞★，女，江苏省建湖县人，汉族，硕士，讲师，主要从事运动与营养方面的研究。yzliuxia@126.com

Protective effect of tea polyphenols on skeletal muscle oxidative injury in acute exhausted rats

Liu Xia   

  1. Department of Traditional Chinese Medical Nutriology, Shanghai University of Traditional Chinese Medicine, Shanghai  201203, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Liu Xia★, Master, Lecturer, Department of Traditional Chinese Medical Nutriology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China yzliuxia@126.com

PDF

354

被引次数

10

摘要：

背景：骨骼肌在急性运动时产生大量自由基，使体内氧化剂与抗氧化剂之间平衡的失调，引起骨骼肌疲劳、肌肉功能障碍和损伤等，使机体运动能力下降。而茶多酚是从茶叶中提取的多酚类物质，具有很强的抗氧化作用，是一种高效低毒的天然抗氧化剂。
目的：通过茶多酚对骨骼肌运动性损伤的干预，观察其对骨骼肌氧化损伤的保护作用。
方法：雄性SD健康大鼠30只，随机分为对照组、运动组、茶多酚组。茶多酚组大鼠补充茶多酚1周后，与运动组大鼠一起通过一次性力竭游泳建立力竭运动模型，记录大鼠力竭时间，测定力竭运动即刻时大鼠骨四头肌中丙二醛，超氧化物歧化酶谷胱甘肽过氧化物酶和总抗氧化能力。
结果与结论：力竭运动使大鼠骨骼肌丙二醛含量增加，超氧化物歧化酶、谷胱甘肽过氧化酶、血浆总抗氧化能力活力下降。茶多酚可以降低运动后丙二醛水平，提高运动后大鼠骨骼肌超氧化物歧化酶、谷胱甘肽过氧化酶、血浆总抗氧化能力活力，延长大鼠力竭时间，由此得出，大鼠力竭运动后骨骼肌组织会发生氧化损伤，茶多酚干预可能会保护骨骼肌氧化损伤，进而提高大鼠运动能力。

关键词: 茶多酚, 骨骼肌, 力竭运动, 自由基, 软组织构建

Abstract:

BACKGROUND: Plenty of free radicals were produced by skeletal muscle after acute exercise, which disturbs the balance between oxidizing agent and antioxidant, leads to skeletal muscle fatigue, muscle dysfunction and damage, eventually decreases the motor ability. Tea polyphenols is a polyphenolic compound extracted from tea and has strong antioxidative activities, which is a natural antioxidant with highly effective and low toxicity.
OBJECTIVE: To intervene the motility damage on skeletal muscle tissue, and to observe the protective effect of tea polyphenols on oxidative injure.
METHODS: Thirty health male Sprague Dawley rats were randomly divided into the control, exercise and tea polyphenols groups. At 1 week after adding tea polyphenols in the tea polyphenols group, rats were prepared for acute exhausted exercise models by exhaustive swimming. The exhaustive duration was recorded, and the levels of malonaldehyde (MDA), super oxide dimutese (SOD), glutathione peroxidase (GSH-Px) and total antioxidative capacity (T-AOC) in skeletal muscle after immediately acute exhausted exercise were measured.
RESULTS AND CONCLUSION: Exhaustive exercise could step up the content of MDA, and significantly decrease SOD, GSH-Px and TAC in rats’ skeletal muscle tissues. While tea polyphenols could decrease MDA levels, increase the SOD, GSH-Px and TAC levels and prolong exhaustive duration of rats. Accordingly, after exhaustive exercise, skeletal muscle tissues were injured by free radicals’ attack. Tea polyphenols can prevent oxdiative damage and improve the ability of exercise.

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