中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6857-6860.doi: 10.3969/j.issn.1673-8225.2010.37.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

骨形态发生蛋白2和血管内皮生长因子mRNA在股骨骨不连大鼠损伤区域的动态表达

徐晓峰1，李  阳1，钱  栋1，马  鹏2，刘小平1，崔学文1   

  1. 江苏大学附属医院，1骨科，2麻醉科，江苏省镇江市 212001　
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 作者简介:徐晓峰，男，1962年生，江苏省南通市人，汉族，1983年南通医学院毕业，主任医师，副教授，硕士研究生导师，主要从事骨组织工程方面的研究。 andy.china@foxmail.com
  • 基金资助:

    镇江市社会发展基金（SH2002019），课题名称：骨髓间充质干细胞体外扩增回植对骨缺损的作用。

Dynamic mRNA expression of bone morphogenetic protein-2 and vascular endothelial growth factor in different regions of rat femoral nonunion

Xu Xiao-feng1, Li Yang1, Qian Dong1, Ma Peng2, Liu Xiao-ping1, Cui Xue-wen1   

  1. 1 Department of Orthopedics, 2 Department of Anesthesiology, Affiliated Hospital of Jiangsu University, Zhenjiang  212001, Jiangsu Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Xu Xiao-feng, Chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China andy.china@foxmail.com
  • Supported by:

    the Foundation for Society Development of Zhenjiang City, No. SH2002019*

PDF

479

被引次数

25

摘要：

背景：骨形态发生蛋白2和血管内皮生长因子可以促进新骨的形成，提高骨不连的治愈率。
目的：观察大鼠股骨骨不连修复过程中骨缺损区骨形态发生蛋白2与血管内皮生长因子mRNA的表达情况。
方法：SD大鼠股骨左建立骨不连模型后，随机分为7组，分别于造模后1，3，7，14，21，28，35 d，处死取材。大鼠右股骨不做缺损直接缝合伤口，作为对照。RT-PCR技术检测大鼠股骨骨形态发生蛋白2和血管内皮生长因子mRNA的表达。
结果与结论：大鼠股骨骨不连损伤后，损伤周围区骨形态发生蛋白2与血管内皮生长因子 mRNA表达上升，至7 d时达到顶峰，而后随时间的延长而下降；而在损伤中心区骨形态发生蛋白2与血管内皮生长因子 mRNA表达延迟，且表达量低于损伤周围区。提示大鼠股骨骨不连缺损中心区域在修复早期骨形态发生蛋白2与血管内皮生长因子mRNA的低表达及时间滞后可能是其骨不连发生的重要原因，在相应的时期补充外源性骨形态发生蛋白2与血管内皮生长因子可能助于骨不连的修复愈合。

关键词: 骨不连, 骨形态发生蛋白2, 血管内皮生长因子, 大鼠, 骨组织工程

Abstract:

BACKGROUND: Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) can promote new born formation, and elevate cure rate of bone nonunion.
OBJECTIVE: To observe the dynamic mRNA expression of BMP-2 and VEGF during restoration of rat femoral nonunion.
METHODS: SD rats were established femoral nonunion models and randomly divided into 7 groups, rats were sacrificed at 1, 3, 7, 14, 21, 28 and 35 days after model preparation. Wounds of rats in the control group were directly sutured without defects. The mRNA expression of BMP-2 and VEGF were detected using RT-PCT technique.
RESULTS AND CONCLUSION: The mRNA expression of BMP-2 and VEGF at the outer zone of injury were increased after injury, reached a peak at 7 days, and the decreased with time prolonged. However, the mRNA expression of BMP-2 and VEGF at the central zone of injury were delayed expressed and smaller than that of the outer zone. Low and delay expression of BMP-2 and VEGF in the central zone during the early stage may be an important reason for bone nonunion, thus, supplying extrinsic source of BMP-2 and VEGF in early stage may be contribute to the repairing of nonunion.

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