中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4271-4274.doi: 10.3969/j.issn.1673-8225.2010.23.021

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

慢肌卫星细胞移植修复骨骼肌的钝挫损伤

胡久婷，朱道立   

  1. 南通大学生命科学学院，江苏省南通市  226007
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 朱道立，教授，南通大学生命科学学院，江苏省南通市 226007 zhudaoli@ ntu.edu.cn
  • 作者简介:胡久婷，女，1988年生，江苏省扬州市人，汉族，南通大学本科在读，主要从事骨骼肌卫星细胞移植的研究。 hjt5902008@ 126.com
  • 基金资助:

    南通大学大学生实践创新训练资助项目(2009042)；
    南通市科技局社会发展科技计划资助项目(S2008022)。

Slow skeletal muscle satellite cells transplantation for repair of skeletal muscle contusion injury

Hu Jiu-ting, Zhu Dao-li   

  1. School of Life Science, Nantong University, Nantong 226007, Jiangsu Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Zhu Dao-li, Professor, School of Life Science, Nantong University, Nantong 226007, Jiangsu Province, China zhudaoli@ntu.edu.cn
  • About author:Hu Jiu-ting, School of Life Science, Nantong University, Nantong 226007, Jiangsu Province, China hjt5902008@126.com
  • Supported by:

    the Practice Innovation Training Program for Undergraduate Student in Nantong University, No. 2009042*;
    the Science and Technology Project for Social Development of Science and Technology Bureau of Nantong City, No. S2008022*

PDF

355

被引次数

2

摘要：

背景：肌卫星细胞在骨骼肌的损伤、修复和维持中起着重要作用，骨骼肌的肌湿质量、肌横切面积及肌动蛋白的含量可为损伤肌肉的恢复提供指标。
目的：观察骨骼肌慢肌卫星细胞移植对肌肉损伤的修复作用。
方法：体外培养分离Sprague-Dawley大鼠慢肌卫星细胞，分别取慢肌卫星细胞悬液和生理盐水(对照组)注入钝挫伤动物模型的损伤部位，观察骨骼肌损伤的修复情况。
结果与结论：移植后实验组比对照组恢复效果好。①卫星细胞移植后5，10，15 d，实验组肌肉组织内荧光标记的肌卫星细胞逐渐减少，形成肌管并最终融合成肌纤维参与损伤修复。②移植后5，10，15 d大鼠肌电图的纤颤电位和正尖波均逐渐减少，且损伤侧的肌电强度和波宽依次增大，逐渐接近正常侧，而对照组肌电强度与波宽则增加缓慢。③随着损伤修复的进程，肌湿质量恢复率均逐渐降低。④随着恢复的进程，实验组灰度值大于对照组数值。提示骨骼肌慢肌卫星细胞移植到钝挫损伤的肌肉内可明显加快损伤肌肉的恢复。

关键词: 卫星细胞, 钝挫伤模型, 比目鱼肌, 移植, 大鼠

Abstract:

BACKGROUND: Muscle satellite cells play an important role in skeletal muscle injury, repair and maintenance. The muscle wet weight, muscle fiber cross-sectional area and the content of actin could provide indicators of the recovery of muscle injury.

OBJECTIVE: To investigate the effect of transplantation of slow skeletal muscle satellite cells on repairing muscle contusion damage at 5, 10, 15 days post surgery.

METHODS: Slow skeletal muscle satellite cells of Sprague-Dawley rat were cultured in vitro. Slow muscle satellite cells and physiological saline (control group) were respectively implanted into injured position of contusion animal model. The repairable conditions of skeletal muscle injury were observed. 

RESULTS AND CONCLUSION: The experimental group post-transplantation obtained good results compared with the control group. ① At 5, 10 and 15 days after the transplantation of satellite cells, satellite cells labeled with fluorescence in muscle tissue gradually reduced in the experimental group. Muscle satellite cells formed myotubes and eventually fused into muscle fibers so as to repair the damage. ②At 5, 10 and 15 days after transplantation, the fibrillation potentials and positive sharp waves were progressively reduced. Myoelectric intensity and pulse width in turn at the injury side were increased by degrees to near normal side, while EMG intensity and pulse width were increased slowly in the control group. ③With the processing of the injured repair, the recovery rates of muscle wet weight were progressively reduced. ④With the processing of the recovery, the gray values of the experimental group were higher than the control group. Results have indicated that the transplantation of slow skeletal muscle satellite cells into the contusion muscles can significantly improve the recovery of muscle contusion damage.

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