中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4239-4242.doi: 10.3969/j.issn.1673-8225.2010.23.014

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脐血间充质干细胞移植后帕金森病大鼠纹状体内多巴胺含量的变化

刘 芳1，樊志刚1，韩雪飞2   

  1. 1河南职工医学院，河南省郑州市  451191；
    2郑州大学医学院干细胞研究中心，河南省郑州市  450052
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 作者简介:刘 芳，女，1976年生，河南省郑州市人，2004年郑州大学毕业，硕士，讲师，主要从事生理学的教学与科研工作。 sincerelf@163. com

Striatal dopamine content in Parkinson’s disease rats following human umbilical cord blood mesenchymal stem cell transplantation

Liu Fang1, Fan Zhi-gang1, Han Xue-fei2   

  1. 1Henan Medical College for Staff and Workers, Zhengzhou   451191, Henan Province, China;
    2Stem Cells Research Center, Medical College of Zhengzhou University, Zhengzhou   450052, Henan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • About author:Liu Fang, Master, Lecturer, Henan Medical College for Staff and Workers, Zhengzhou 451191, Henan Province, China sincerelf @163.com

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488

被引次数

15

摘要：

背景：目前干细胞移植对帕金森病动物模型的研究多集中在骨髓间充质干细胞和胚胎干细胞方面，关于脐血间充质干细胞移植对帕金森病动物模型的研究相对较少，且未见测量脐血间充质干细胞移植前后多巴胺含量变化的报道。
目的：观察脐血干细胞移植对帕金森病大鼠纹状体内多巴胺含量的影响。
方法：帕金森病模型大鼠随机分成实验组和对照组。将第3代脐血间充质干细胞用Hoechst33258标记后植入实验组大鼠纹状体内，对照组注射磷酸盐缓冲溶液。移植后2，4，8周用免疫荧光双标法检测间充质干细胞的存活、迁移以及胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶和突触素的表达。移植后8周利用高效液相色谱-电化学检测仪检测纹状体多巴胺含量。
结果与结论：脐血间充质干细胞移植后可在大鼠脑内存活，随时间延长迁移范围扩大，分布于纹状体、胼胝体和皮质；胶质纤维酸性蛋白、神经元特异性烯醇化酶、酪氨酸羟化酶都有表达，突触素无表达。多巴胺水平与对照组相比有明显提高   (P < 0.05)。

关键词: 脐血间充质干细胞, 细胞移植, 帕金森病, 多巴胺, 大鼠

Abstract:

BACKGROUND: At present, researches of stem cell transplantation for Parkinson’s disease (PD) animal model mainly utilize bone marrow mesenchymal stem cells (MSCs) and embryonic stem cells. Researches of umbilical cord blood MSCs transplantation are relatively less, and there is no report regarding the change of striatal dopamine content before and after umbilical cord blood MSC transplantation.

OBJECTIVE: To explore the effect of human umbilical cord blood MSCs transplantation on the dopamine content in PD rat striatum.

METHODS: The PD rat models were randomly divided into experimental (n=20) and control (n=20) groups. The third passage MSCs were marked by Hoechst33258, and stereotactically transplanted into experimental group, and the control group was given PBS. The brain tissue of PD rat was sectioned to identify the survival and migration of MSCs and the expression of neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP), tyrosine hydroxylase (TH), and synaptophysin under fluorescent microscope at weeks 2, 4 and 8. Dopamine in striatum was detected by high performance liquid chromatography at 8 weeks of transplantation.

RESULTS AND CONCLUSION: Transplanted MSCs survived in the brain, distributed in striatum, callus, and cortex with increasing time, and expressed NSE, GFAP, and TH, but not synapsin. The content of dopamine was significantly increased than control group (P < 0. 05).

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