中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1319-1321.doi: 10.3969/j.issn.1673-8225.2010.07.042

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

组织蛋白酶K在人椎间盘中的表达及意义

刘  雅，王宗亮，蔡  明，王  敏，王  尚   

  1. 南阳市骨科医院脊柱外科，河南省南阳市  473000
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 作者简介:刘 雅，男，1963年生，河南省南阳市人，汉族，1981年新乡医学院毕业，副主任医师，主要从事脊柱外科研究。

Expression and clinical significance of cathepsin K in intervertebral disk degeneration in humans

Liu Ya, Wang Zong-liang, Cai Ming, Wang Min, Wang Shang   

  1. Department of Spine Surgery, Orthopedics Hospital of Nanyang, Nanyang   473000, Henan Province, China
  • Online:2010-02-12 Published:2010-02-12
  • About author:Liu Ya, Associate chief physician, Department of Spine Surgery, Orthopedics Hospital of Nanyang, Nanyang 473000, Henan Province, China wzl21@126.com

PDF

509

被引次数

4

摘要：

背景: 研究证实，椎间盘内酶类的激活与基质成分的降解密切相关。已知基质金属蛋白酶和金属蛋白酶组织抑制剂两个酶系统在椎间盘细胞外基质改变过程中发挥着重要作用，除两者外，其他对细胞外基质有降解作用的蛋白酶是否也参与了椎间盘的退变过程？
目的：检测正常和退变人椎间盘细胞中组织蛋白酶K的表达，探讨组织蛋白酶K与椎间盘退变的相关性。
方法：应用免疫组化S-P法和ELISA法对30例腰椎间盘突出症患者椎间盘组织中组织蛋白酶K进行检测,并取15例健康成人(尸体)和脊柱骨折手术患者正常椎间盘组织作对照，观察组织蛋白酶K在正常和退变人椎间盘细胞中的蛋白表达。
结果与结论：正常椎间盘和退变椎间盘中均有组织蛋白酶K表达,退变椎间盘中组织蛋白酶K表达水平明显高于正常组,差异有显著性意义( P < 0.05)。提示组织蛋白酶K可能参与了椎间盘退变的病理改变。

关键词: 组织蛋白酶K, 椎间盘, 退变, 基质, 骨组织工程

Abstract:

BACKGROUND: Several studies have confirmed that activation of intervertebral disc enzymes is closely related to matrix degradation. Matrix metalloproteinase and tissue inhibitor of metalloproteinase have been shown to exert important roles in the process of extracellular matrix degeneration in intervertebral disk. Besides these two enzyme systems, whether other proteases that exhibit degrading effects on extracellular matrix are involved in the intervertebral disk degeneration remains poorly understood. 
OBJECTIVE: To detect the cathepsin K expression in normal and degenerated human intervertebral disc cells and investigate the correlation between cathepsin K and intervertebral disc degeneration.
METHODS: Cathepsin K expression was detected in intervertebral disc tissue from 30 patients with lumbar intervertebral disc protrusion using immunohistochemistry SP method and ELISA. At the same time, the intervertebral disc tissue from 15 healthy adult cadavers and/or spine fracture patients was taken as control. Cathepsin K protein expression in normal and degenerated human intervertebral disc tissues were compared.
RESULTS AND CONCLUSION: Cathepsin K expression was observed in normal and degenerated intervertebral disc tissues. The expression level was significantly higher in degenerated tissue than normal tissue (P < 0.05). These findings demonstrate that Cathepsin K possibly participates in the intervertebral disc degeneration.

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