中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 433-437.doi: 10.3969/j.issn.1673-8225.2010.03.013

• 药物控释材料 drug delivery materials • 上一篇    下一篇

静电贴附海藻酸钙微球制备自组装支架

陈文斌1，2，鲁  路1，2，焦延鹏1，2，周长忍1，2   

  1. 1暨南大学理工学院材料系，广东省广州市  510632；2人工器官及材料教育部工程研究中心，广东省广州市  510632
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 周长忍，教授，博士生导师，暨南大学材料科学与工程系，广东省广州市 510632；人工器官及材料教育部工程中心，广东省广州市 510630 tcrz9@jnu.edu.cn
  • 作者简介:陈文斌★，男，1983年生，湖南省永州市人，暨南大学在读硕士，主要从事生物材料方面的研究。 cwb2217@sina.com
  • 基金资助:

    国家高新技术发展计划(863计划)(2007AA091603)。课题名称：功能性壳聚糖基组织器官修复材料的产业化技术研究。

Preparation of self-assembly scaffolds via electrostatic attachment of calcium alginate microspheres

Chen Wen-bin 1,2, Lu Lu 1, 2 , Jiao Yan-peng 1, 2 , Zhou Chang-ren 1, 2   

  1. 1 Department of Material Science and Engineering, Jinan University, Guangzhou  510630, Guangdong Province, China; 2 Engineering Research Center of Artificial Organ and Materials, Ministry of Education, Guangzhou  510630, Guangdong Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Zhou Chang-ren, Professor, Doctoral supervisor, Department of Material Science and Engineering, Jinan University, Guangzhou 510630, Guangdong Province, China; Engineering Research Center of Artificial Organ and Materials, Ministry of Education, Guangzhou 510630, Guangdong Province, China tcrz9@jnu.edu.cn
  • About author:Chen Wen-bin★, Studying for master’s degree, Department of Material Science and Engineering, Jinan University, Guangzhou 510630, Guangdong Province, China; Engineering Research Center of Artificial Organ and Materials, Ministry of Education, Guangzhou 510630, Guangdong Province, China cwb2217@sina.com
  • Supported by:

    the National High Technology Research and Development Program (863 Program) of China, No. 2007AA091603*

PDF

637

被引次数

4

摘要：

背景：微球型注射支架在软骨组织工程中具有良好的发展前景，但是常存在体内成型困难和微球游走等问题。
目的：探讨利用静电作用力将负电性海藻酸钙微球与正电性壳聚糖微球贴附在一起制备自组装支架的可行性。
方法：用乳化内部凝胶法制备表面带负电荷的海藻酸钙微球，用喷雾干燥法制备表面带正电荷的壳聚糖微球。扫描电镜观察微球的表面形貌、光学显微镜分析微球的粒径及其粒径分布，zeta电位仪测定微球的表面电位；将两种带电荷微球的悬浮液混合在一起制备自组装支架，用光学显微镜和扫描电镜观察微球间的静电贴附情况，并对支架的压缩弹性模量进行了分析。
结果与结论：海藻酸钙微球的平均粒径为52.5 μm，表面电位为-23.5 mV，壳聚糖微球的平均粒径为4.1 μm，表面电位为+9.8 mV，两种微球表面光滑，成球性良好；光学显微镜和扫描电镜下可以观察到小粒径的壳聚糖微球能够将海藻酸钙微球贴附在一起，支架的压缩弹性模量随微球固含量的增加而增加，随溶液离子强度的增加而减小，随壳聚糖微球与海藻酸钙微球质量比的增加，先增加后减小，当壳聚糖微球与海藻酸钙微球质量比为2∶1时支架的压缩弹性模量最佳。提示正电性的壳聚糖微球可将负电性的海藻酸钙微球贴附在一起而形成自组装型支架。

关键词: 静电作用, 海藻酸钙, 壳聚糖, 贴附, 自组装

Abstract:

BACKGROUND: Microspheric injectable scaffold has a perspective in cartilage tissue engineering; however, it is still limited by in vivo hard forming and microsphere transmigration. 
OBJECTIVE: To investigate the feasibility of self-assembly scaffolds by attaching negatively charged calcium alginate microspheres and positively charged chitosan microspheres by electrostatic force.
METHODS: The calcium alginate and chitosan microspheres were prepared by emulsion-internal gelation and spray drying technique, respectively. The characterizations of the microspheres were determined by means of scanning electron microscopy, optical microscopy and zeta potential analysis methods. Self-assembly scaffolds were fabricated by mixing the aqueous suspensions of the microspheres with oppositely charged surfaces. The phenomenon of electrostatic attachment was characterized by optical microscopy and scanning electron microscopy, and the elastic compress modulus of the scaffolds was also investigated.
RESULTS AND CONCLUSION: The average diameter of the calcium alginate microspheres was 52.5 μm, and the chitosan one was 4.1 μm, respectively. The zeta-potential of the calcium alginate microspheres was -23.5 mv, and the chitosan one was +9.8 mv, respectively. The microspheres were spherical and smooth. The small size chitosan microspheres could attach to the surface of the calcium alginate microspheres and anchor the calcium alginate microspheres together. The elastic compress modulus increased with the increase of solid content of the microspheres, but decreased with the increase of the ionic strength. The elastic compress modulus increased firstly and then decreased with the increase of the mass ratio of m (CHI):m (ALG), and it showed the highest elastic compress modulus when m (CHI):m (ALG) was 2:1. The positively charged chitosan microspheres could attach to the negatively calcium alginate microspheres to form a self-assembly scaffold.

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