中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 429-432.doi: 10.3969/j.issn.1673-8225.2010.03.012

• 药物控释材料 drug delivery materials • 上一篇    下一篇

壳寡糖对Hela细胞cyclin D1、bcl-xl和bcl-2 mRNA表达的影响

李  贤1，汪  炬2，周长忍1，田金环1，陈小佳2，赵名艳1   

  1. 1暨南大学理工学院材料科学与工程系，广东省广州市 510632; 2暨南大学生物工程研究所，广东省广州市  510632
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 周长忍，教授，博士生导师，暨南大学理工学院材料科学与工程系，广东省广州市 510632 tcrz9@jnu.edu.cn
  • 作者简介:李 贤☆，女，1975年生，汉族，河北省保定市人，暨南大学在读博士，主要从事生物材料与细胞相互作用方面的研究。 lxchina2006@163.com
  • 基金资助:

    国家高新技术发展计划(863计划)，编号：2007AA091603。课题名称：功能性壳聚糖基组织器官修复材料的产业化技术研究。

Effect of chitooligosaccharides on cyclin D1, bcl-xl and bcl-2 mRNA expression in Hela cells

Li Xian1, Wang Ju2, Zhou Chang-ren1, Tian Jin-huan1, Chen Xiao-jia2, Zhao Ming-yan1   

  1. 1 Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou  510632, Guangdong Province, China; 2 Bio-engineering Institute of Jinan University, Guangzhou     510632, Guangdong Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Zhou Chang-ren, Professor, Doctoral supervisor, Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou 510632, Guangdong Province, China tcrz9@jnu.edu.cn
  • About author:Li Xian☆, Studying for doctorate, Department of Materials Science and Engineering, School of Technology, Jinan University, Guangzhou 510632, Guangdong Province, China lxchina2006@163.com
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2007AA091603*

PDF

946

被引次数

24

摘要：

背景：研究表明壳寡糖具有抗肿瘤作用，但壳寡糖对cyclin D1、bcl-2和bcl-xl影响机制尚不清楚。
目的：观察壳寡糖抑制Hela细胞生长的作用，以及对细胞内cyclin D1、bcl-xl和 bcl-2 mRNA表达的影响。
方法：壳寡糖(0.1，0.5，1，2，5 g/L)刺激Hela细胞后，CCK8实验检测其对细胞生长的影响；运用实时荧光定量PCR方法在分子水平检测cyclin D1、bcl-xl和 bcl-2 mRNA在细胞中的表达情况。
结果与结论：壳寡糖可抑制Hela细胞的生长；随着壳寡糖的质量浓度由0.1 g/L增加至2 g/L，壳寡糖抑制cyclin D1，bcl-2，bcl-xl基因表达的作用也逐渐增强，在2 g/L时作用最强，但质量浓度过高时(5 g/L)抑制作用反而有所减弱。结果表明壳寡糖的抗肿瘤作用可能通过两方面进行：一方面壳寡糖可以抑制与细胞周期有关的cyclin D1 mRNA表达以抑制肿瘤细胞的增殖；另一方面壳寡糖可以抑制抗凋亡蛋白Bcl-2 和bcl-xl mRNA的表达以促进肿瘤细胞的凋亡；且对前者的作用强于后者。

关键词: 荧光定量PCR, 壳寡糖, Hela细胞, 细胞周期蛋白 D1, bcl-xl, bcl-2

Abstract:

BACKGROUND: Studies have shown that chitooligosaccharides have antitumor effect. However, the influence of chitooligosaccharides on cyclin D1, bcl-2 and bcl-xl remains unclear.
OBJECTIVE: To observe the inhibition effect of chitooligosaccharides on the proliferation of Hela cells, and the influence on cyclin D1, bcl-xl and bcl-2 mRNA expression.
METHODS: Hela cells were stimulated by different concentrations of chitooligosaccharides (0.1, 0.5, 1, 2, 5 g/L). The effects of chitooligosaccharides on Hela cells were detected by CCK8 kit. Using real-time PCR methods, the gene expression of cyclin D1, bcl-xl and bcl-2 mRNA were determined.
RESULTS AND CONCLUSION: Chitooligosaccharides inhibited the proliferation of Hela cells. With the concentrations of chitooligosaccharides increased from 0.1 g/L to 2 g/L, the inhibition effects on the gene expression of cyclin D1, bcl-xl and bcl-2 were enhanced, peaked at 2 g/L, and decreased at high concentration (5 g/L). Antitumor activity of chitooligosaccharides may exert through two aspects: it inhibits cyclin D1 mRNA expression to suppress the proliferation of tumor cells; on the other hand, chitooligosaccharides inhibits the expression of bcl-xl and bcl-2 to promote the apoptosis of tumor cells. Moreover, the effects of the former are stronger than the latter.

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