中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 400-404.doi: 10.3969/j.issn.1673-8225.2010.03.006

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

拉伸-复形过程对骨修复形状记忆聚氨酯材料细胞相容性的影响

颜泽萱，罗彦凤，黄美娜，王远亮   

  1. 教育部生物流变科学与技术重点实验室(重庆大学)，重庆大学生物工程学院生物材料与仿生工程研究中心，重庆市 400030
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 罗彦凤，博士，教授，博士生导师，教育部生物流变科学与技术重点实验室（重庆大学），重庆大学生物工程学院生物材料与仿生工程研究中心，重庆市 400030 yfluo@cqu.edu.cn
  • 作者简介:颜泽萱★，女，1983年生，重庆市人，汉族，2006年重庆大学毕业，硕士，主要从事细胞/组织工程与生物材料方面的研究。 yan_zexuan@163.com
  • 基金资助:

    国家“十一五”科技支撑计划项目(2006BA103B04)，课题名称“避孕节育新技术和新方法的研究”；重庆市科技攻关项目(CSTC 2008AB0027)，课题名称“基于形状记忆聚合物的输卵管绝育栓的研制”。

Effects of stretching-shape recovering process on the cytocompatibility of novel shape memory polyurethane for bone repair

Yan Ze-xuan, Luo Yan-feng, Huang Mei-na, Wang Yuan-liang   

  1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Research Center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing   400030, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Luo Yan-feng, Doctor, Professor, Doctoral supervisor, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Research Center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China yfluo@cqu.edu.cn
  • About author:Yan Ze-xuan★, Master, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Research Center of Bioinspired Material Science and Engineering, College of Bioengineering, Chongqing University, Chongqing 400030, China yan_zexuan@163.com
  • Supported by:

    the National Technology Supporting Project of China during the 11th Five-Year Plan Period, No. 2006BA103B04*; the Tackle Key Program in Science and Technology of Chongqing City, No. CSTC 2008AB0027*

PDF

605

被引次数

15

摘要：

背景：形状记忆聚氨酯的温敏性形状记忆功能使其可用于缺损骨的修复，达到抗应力遮挡和抗骨不连的作用。准确的细胞相容性评价对于形状记忆聚氨酯进一步的体内实验和应用具有重要的指导意义，但目前关于变形-复形过程对形状记忆聚氨酯细胞相容性的影响报道甚少。
目的：考察形状记忆聚氨酯在变形-复形前后的成骨细胞相容性。
方法：采用溶剂浇注成膜法制备形状记忆聚氨酯膜，分别在Tg+15 ℃、Tg-15 ℃和Tg+15 ℃条件下对形状记忆聚氨酯膜进行拉伸变形(拉伸率200%)、固形和复形，制得拉伸-复形后的形状记忆聚氨酯膜。采用轻敲模式原子力显微镜观察形状记忆聚氨酯膜表面的相分离和表面形貌。将体外培养的第3~5代成骨细胞直接接种到形状记忆聚氨酯膜上，检测成骨细胞的黏附、增殖和铺展情况。
结果与结论：形状记忆聚氨酯膜在拉伸-复形前后都具有明显而规整的相分离，拉伸-复形过程在形状记忆聚氨酯膜表面形成较规整的微米级的“沟槽-脊”结构。与拉伸-复形前的形状记忆聚氨酯相比，成骨细胞在这种具有微米级“沟槽-脊”结构的表面上可以更好的黏附和增殖，且成骨细胞的排列表现出一定的取向性。结果表明拉伸-复形过程可明显改变形状记忆聚氨酯膜的表面形貌，并表现出更好的与成骨细胞的生物相容性。

关键词: 形状记忆聚合物, 聚氨酯, 细胞相容性, 骨修复, 生物材料

Abstract:

BACKGROUND: Shape memory polyurethane (SMPU) may be employed for bone repair capable of resisting stress shielding and bone non-union due to the shape memory effect responding to changed external temperature. Evaluating the cytocompatibility of SMPU is important for its further in vivo experiments and applications. However, few have been done to investigate the cytocompatibility of SMPU after encounted from deforming and shape recovering.
OBJECTIVE: To evaluate the osteoblast compatibility of SMPU before and after stretching-shape recovering process.
METHODS: Solvent casting method was used to fabricate SMPU films; the obtained SMPU films were stretched to 200%, and then fixed and finally recovered to its original shape at Tg+15 ℃, Tg-15 ℃ and Tg+15 ℃, respectively. Atomic force microscope (AFM) with tapping mode was employed to probe the surface morphology and phase separation of SMPU. Primary osteoblasts at 3-5 passages were seeded on SMPU films in vitro to evaluate the adhesion, proliferation and spreading of osteoblasts.
RESULTS AND CONCLUSION: There were obvious and regular phase separation resulted from soft segments and hard segments in SMPU, and some groove-ridge architectures within a scale of micrometers were produced by the stretching-shape recovering process. These special micropatterned structures promoted osteoblast adhesion and proliferation, and also resulted in partially oriented cell growth along the grooves. Shape memory process, i.e. stretching-shape recovering process may obviously change the surface morphology of SMPU films, and suggesting better biocompatibility with osteoblasts.

中图分类号: