中国组织工程研究

中国组织工程研究 ›› 2024, Vol. 28 ›› Issue (5): 712-716.doi: 10.12307/2024.264

• 材料力学及表面改性 material mechanics and surface modification • 上一篇    下一篇

不同含量B2O3对生物活性玻璃支架力学性能与生物活性的影响

王建春1，杨树青2，苏  欣2，王宏远2   

  1. 1赤峰学院附属医院，内蒙古自治区赤峰市  024000；2内蒙古医科大学第四附属医院口腔科，内蒙古自治区包头市  014032
  • 收稿日期:2022-11-03 接受日期:2023-04-18 出版日期:2024-02-18 发布日期:2023-08-16
  • 通讯作者: 王宏远，主任医师，硕士生导师，内蒙古医科大学第四附属医院口腔科，内蒙古自治区包头市 014032
  • 作者简介:王建春，男，1996年生，内蒙古自治区赤峰市人，蒙古族，硕士，医师，主要从事颌骨修复材料研究。
  • 基金资助:
    内蒙古自然科学基金课题项目(2019MS08177)，项目负责人：王宏远

Different contents of B2O3 affect mechanical properties and bioactivity of bioactive glass scaffolds

Wang Jianchun1, Yang Shuqing2, Su Xin2, Wang Hongyuan2   

  1. 1Affiliated Hospital of Chifeng University, Chifeng 024000, Inner Mongolia Autonomous Region, China; 2Department of Stomatology, Fourth Affiliated Hospital of Inner Mongolia Medical University, Baotou 014032, Inner Mongolia Autonomous Region, China
  • Received:2022-11-03 Accepted:2023-04-18 Online:2024-02-18 Published:2023-08-16
  • Contact: Wang Hongyuan, Chief physician, Master’s supervisor, Department of Stomatology, Fourth Affiliated Hospital of Inner Mongolia Medical University, Baotou 014032, Inner Mongolia Autonomous Region, China
  • About author:Wang Jianchun, Master, Physician, Affiliated Hospital of Chifeng University, Chifeng 024000, Inner Mongolia Autonomous Region, China
  • Supported by:
    Natural Science Foundation Project of Inner Mongolia Autonomous Region, No. 2019MS08177 (to WHY)

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摘要：


文题释义：

氮氧生物活性玻璃：是一种以硅酸盐类CaO-SiO2为基础的人工骨修复替代材料，由硅、钙、磷和钠的氧化物组成CaO-SiO2-P2O5系统，最早由 HENCH发现并命名，而氮氧生物活性玻璃是对基础玻璃进行过氮化处理，提高了其力学性能。
人工合成骨替代材料：此类研发的骨替代生物材料拥有诸多优点，被植入到骨缺损部位后能为新骨的形成提供理想的机体微环境，同时使成骨细胞拥有一个可依附的稳定三维空间，且原料易获取、成本低廉。


背景：生物活性玻璃骨修复材料具有骨结合能力、骨诱导能力及骨传导特性，但目前生物活性玻璃的性能尚不符合临床应用要求，添加硼元素有望改善生物活性玻璃的性能。

目的：研究不同含量B2O3替代SiO2对生物活性玻璃力学性能及生物活性的影响。
方法：以含磷氮氧生物活性玻璃(成分为：SiO2-CaO-ZnO-Na2O-Si3N4-P2O5)为基础，以B2O3部分替代其中的SiO2，采用高温熔融法烧制含B2O3质量分数分别为0%(A组)，5%(B组)，10%(C组)，15%(D组)的基础玻璃(基础玻璃中SiO2与B2O3的质量分数总和为41%)，采用有机泡沫浸渍法制作多孔生物活性玻璃支架，利用万能力学试验机单轴压缩和三点弯曲法测试力学性能；将4组支架浸泡于模拟体液中，检测支架的降解性能，利用扫描电镜观察浸泡前后支架的形貌变化，以及X射线衍射分析浸泡前后的支架物相组成。

结果与结论：①随着B2O3质量分数的增加，多孔生物活性玻璃支架的抗压强度与抗弯强度升高，4组支架的抗压强度与抗弯强度比较差异有显著性意义(P ≤ 0.05)；②浸泡于模拟体液中后，随着时间的延长，多孔生物活性玻璃支架逐渐降解；相同浸泡时间点下，随着B2O3质量分数的增加，支架的降解速率加快，4组支架的抗压强度与抗弯强度比较差异有显著性意义(P ≤ 0.05)；③浸泡于模拟体液后的扫描电镜显示，A、B组浸泡1 d后表面沉积大量的颗粒状物质，3 d后表面的颗粒状物质相互融合形成薄膜样沉积，7 d后表面的薄膜即相互融合成片，基本覆盖整个试件表面；C组浸泡1 d后表面形成薄膜样物质沉积，3 d后表面的薄膜即相互融合成片，基本覆盖整个试件表面；D组浸泡1 d后可见基本覆盖整个试件表面的片状物质；④浸泡于模拟体液中1 d后的X射线衍射分析显示，4组支架表面的沉积物为结晶态的羟基磷灰石；⑤B2O3替代部分SiO2会增强多孔生物活性玻璃支架的力学性能、降解性能及体外矿化活性。

https://orcid.org/0000-0001-9458-928X(王建春)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 生物活性玻璃, 骨修复材料, 支架, 力学性能, 生物活性

Abstract: BACKGROUND: Bioactive glass bone repair material has bone-bonding ability, bone induction ability and bone conduction characteristics. However, the performance of bioactive glass does not meet the requirements of clinical application, and the addition of boron is expected to improve the performance of bioactive glass.
OBJECTIVE: To study the effect of different contents of B2O3 replacing SiO2 on the mechanical properties and bioactivity of bioactive glass.
METHODS: Based on bioactive glass containing phosphorus nitrogen and oxygen (composition: SiO2-CaO-ZnO-Na2O-Si3N4-P2O5), B2O3 was used to partially replace the SiO2. The basic glass containing B2O3 with a mass fraction of 0% (group A), 5% (group B), 10% (group C), and 15% (group D) was fired using the high-temperature melting method (the total mass fraction of SiO2 and B2O3 in the basic broken glass was 41%). Porous bioactive glass scaffolds were fabricated by the organic foam impregnation method. Uniaxial compression and three-point bending method of universal mechanical testing machine were used to test mechanical properties. Four groups of scaffolds were immersed in simulated body fluids to detect the degradation performance of scaffolds. Scanning electron microscopy was used to observe the morphological changes of scaffolds before and after soaking. X-ray diffraction was used to analyze the phase composition of scaffolds before and after soaking.
RESULTS AND CONCLUSION: (1) With the increase of the mass fraction of B2O3, the compressive strength and bending strength of the porous bioactive glass scaffold increased, and there was a significant difference between the compressive strength and bending strength of the four groups (P ≤ 0.05). (2) After soaking in simulated body fluids, the porous bioactive glass scaffolds degraded gradually with the extension of time. At the same soaking time point, the degradation rate of the scaffolds was accelerated with the increase of the mass fraction of B2O3, and the compressive strength and bending strength of the scaffolds in the four groups were significantly different (P ≤ 0.05). (3) Scanning electron microscopy after soaking in simulated body fluids showed that a large number of granular materials were deposited on the surface of group A and group B after soaking for 1 day. After 3 days, the granular materials on the surface fused with each other to form film-like deposits. After 7 days, the films on the surface fused with each other to form pieces, basically covering the entire surface of the specimen. After soaking for 1 day, film-like material deposition was formed on the surface of group C, and after 3 days, the films on the surface were fused into pieces, basically covering the whole surface of the specimen. After soaking for 1 day in group D, flake material covering the whole surface of the specimen could be seen. (4) X-ray diffraction analysis after 1 day of immersion in simulated body fluids showed that the deposits on the surface of the four groups of scaffolds were crystallized hydroxyapatite. (5) B2O3 replacement of SiO2 can enhance the mechanical properties, degradation properties and in vitro mineralization activity of porous bioactive glass scaffolds.

Key words: bioactive glass, bone repair material, scaffold, mechanical property, bioactivity

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