关键词: 脂肪细胞肿瘤, 苏木素, 伊红, 非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤, MDM2, 基因

Abstract: BACKGROUND: The quality of conventional paraffin sections prepared by traditional reagents (formaldehyde solution fixation, ethanol dehydration, xylene transparent dewaxing) used for tissue pretreatment is low, which has become the bottleneck of pathological diagnosis of adipocytic tumors. Moreover, traditional reagents are easy to pollute the laboratory environment and cause various health hazards to laboratory personnel.
OBJECTIVE: To explore the application value of environmental friendly tissue sample preparation solution in the detection of MDM2 gene in adipocyte-tumor hematoxylin-eosin staining and atypical lipomatous-tumor/well differentiated liposarcoma.
METHODS: From February 2016 to July 2022, 592 samples of adipocyte tumors were selected as the research objects. The same sample was cut in half. According to the different reagents used in the pretreatment of samples, they were randomly divided into two groups: the traditional group and the environmental protection group. In the traditional group, 592 conventional paraffin sections were made by formalin fixation, alcohol dehydration and xylene transparent dewaxing. In the environmental protection group, 592 slices were made with environmental protection tissue sample preparation solution. According to the different grades of hematoxylin-eosin staining quality of sections, the excellent and good rate of the matoxylin-eosin staining was compared between the two groups. Further, 33 specimens of atypical lipomatous tumor/well differentiated liposarcoma confirmed by pathology were sectioned again, and the MDM2 gene was detected by fluorescence in situ hybridization to compare the difference in the amplification rate of MDM2 gene between the two groups.
RESULTS AND CONCLUSION: (1) Compared with the traditional group, the tissue sections of the adipocyte tumors in the environmental protection group were more stretched and complete; the cells were not folded; the staining was clearer; the red blue contrast was better and the cell structure was denser. (2) The excellent and good rate of hematoxylin-eosin staining of tissue sections in the environmental protection group was higher than that in the traditional group, with a statistically significant difference (P=0.000). (3) Compared with the traditional group, the MDM2 probe and the CSP12 probe of the environmental protection group bound only in the specific region of the sample chromosome, which was more specific. (4) The number of successfully hybridized cells in the environmental protection group was higher than that in the traditional group, and the difference was statistically significant (P=0.000). (5) There was no significant difference in MDM2 gene signal number, MDM2/cell value, CSP12 signal number, CSP12/cell value and MDM2/CSP12 value between the traditional group and the environmental protection group (P > 0.05). (6) There was no significant difference in the amplification rate of MDM2 gene between the two groups (P=0.31). (7) The results showed that the environment-friendly tissue sample preparation solution is conducive to improving the excellent and good rate of hematoxylin-eosin staining of adipocyte tumors and ensuring the detection quality of MDM2 gene in atypical lipomatous tumors/well differentiated liposarcomas and has the value of clinical promotion. 

Key words: adipocyte tumor, hematoxylin, eosin, atypical lipomatous tumor/well differentiated liposarcoma, MDM2, gene