中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (12): 1832-1836.doi: 10.12307/2023.005

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

茶多酚处理脱细胞牛心包组织支架作为人工食管修补材料的可行性

李  芹，孙扬永，吴  昊，刘晓红，徐志云   

  1. 上海长海医院心血管外科，上海市  200433
  • 收稿日期:2021-09-08 接受日期:2021-12-07 出版日期:2023-04-28 发布日期:2022-07-30
  • 通讯作者: 刘晓红，副教授，上海长海医院心血管外科实验中心，上海市 200433 徐志云，教授，上海长海医院心血管外科，上海市 200433
  • 作者简介:李芹，女，安徽省宿州市人，汉族，医学检验技师，主要从事生物工程材料研究。
  • 基金资助:
    科技部国家重点研发计划(2016YFC1100900) ，项目负责人：徐志云

Feasibility of tea polyphenol-treated bovine pericardial decellularized tissue scaffolds as artificial esophageal repair materials

Li Qin, Sun Yangyong, Wu Hao, Liu Xiaohong, Xu Zhiyun   

  1. Department of Cardiovascular Surgery, Changhai Hospital, Shanghai 200433, China
  • Received:2021-09-08 Accepted:2021-12-07 Online:2023-04-28 Published:2022-07-30
  • Contact: Liu Xiaohong, Associate professor, Department of Cardiovascular Surgery, Changhai Hospital, Shanghai 200433, China Xu Zhiyun, Professor, Department of Cardiovascular Surgery, Changhai Hospital, Shanghai 200433, China
  • About author:Li Qin, Medical Laboratory Technician, Department of Cardiovascular Surgery, Changhai Hospital, Shanghai 200433, China
  • Supported by:
    National Key Research and Development Program of the Ministry of Science and Technology, No. 2016YFC1100900 (to XZY)

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摘要：

文题释义：
脱细胞牛心包组织支架：牛心包为常见的生物组织材料，去除牛心包的细胞成分后可降低其免疫原性，同时保留机械和生物活性特性，在临床上得到了广泛应用。
茶多酚：又称绿茶提取物，主要是从绿茶中提取的一种天然多酚类化合物，主要成分为儿茶素。茶多酚对癌症、糖尿病、神经系统疾病、心血管疾病等具有多重保护作用，且其交联后的牛心包具有良好的相容性，可促进细胞浸润与血管组织新生，有利于组织再生。

背景：脱细胞组织支架已被应用于心脏、血管、皮肤、肺部和泌尿科等领域，并逐渐成为食管组织工程的良好候选者。
目的：探讨茶多酚处理脱细胞牛心包组织支架作为人工食管材料的可行性。
方法：制备脱细胞牛心包组织支架，分别经PBS、戊二醛、茶多酚进行交联处理。将16只SD大鼠随机分4组，A组将经PBS处理的脱细胞牛心包组织支架缝合至食管缺损处，B组将经戊二醛处理的脱细胞牛心包组织支架缝合至食管缺损处，C组将经茶多酚处理的脱细胞牛心包组织支架缝合至食管缺损处，D组暴露食管后关腹，每组4只。术后4周，观察大鼠体质量变化、吻合口情况，修复部位苏木精-伊红染色及肌动蛋白α抗体免疫组化染色。
结果与结论：①大体观察可见，A组食管缺损部位肉芽增生，狭窄不明显，部分食管补片材料脱落；B组术后出现吻合口漏1只，食管缺损部位肉芽组织增生明显，大块补片组织突出于食管腔内；C组食管缺损部位肉芽组织增生，缺损部位被人工食管支撑，无明显狭窄；②A、B、C组大鼠术后4周的体质量与D组比较差异无显著性意义(P > 0.05)；③苏木精-伊红染色显示，A组食管缺损部位肉芽增生，大量炎性细胞浸润，新生食管黏膜部分覆盖补片组织；B组食管缺损部位肉芽组织增生明显，伴有大量炎性细胞浸润，补片周边可见炎性细胞浸润，食管黏膜未覆盖补片组织；C组食管缺损部位肉芽组织增生，炎性细胞浸润较轻，人工食管补片内可见大量新生细胞，原补片组织结构不清，食管黏膜完全覆盖缺损组织；④免疫组化染色显示，A、B组未见肌动蛋白α阳性染色细胞，C组可见蓝染的细胞核，原补片结构疏松，可见肌动蛋白α特异性染色的新生血管组织；⑤结果表明，茶多酚处理脱细胞牛心包组织支架可作为人工食管修复材料。

https://orcid.org/0000-0003-1004-7390 (李芹)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 人工食管, 食管破裂, 茶多酚, 牛心包, 脱细胞组织支架, 交联

Abstract: BACKGROUND: Decellularized tissue scaffolds have been used in vascular, cardiovascular, skin, pulmonary, and urology fields, and have gradually become good candidates for esophageal tissue engineering.
OBJECTIVE: To investigate the feasibility of tea polyphenol-treated bovine pericardial decellularized tissue scaffolds as artificial esophagus materials. 
METHODS: The bovine pericardium was decellularized and made into tissue scaffolds, which were treated with PBS, glutaraldehyde or tea polyphenol. Totally 16 SD rats were randomly assigned to four groups (n=4). In group A, PBS-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects. In group B, glutaraldehyde-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects. In group C, tea polyphenol-treated decellularized bovine pericardial tissue scaffolds were sutured to esophageal defects. In group D, the abdomen was sutured after exposing the esophagus. At 4 weeks after the operation, the changes in body weight, anastomotic stoma, hematoxylin-eosin staining and actin α antibody immunohistochemical staining at the repaired site were observed.
RESULTS AND CONCLUSION: (1) The general observation showed that in group A, the esophageal defect site had granulation hyperplasia; the stenosis was not obvious; and part of the esophageal patch material fell off. In group B, one patient had anastomotic leakage after operation. The granulation tissue in the esophageal defect site proliferated obviously and the large patch tissue protruded into the esophageal cavity. In group C, granulation tissue hyperplasia was found at the esophageal defect site; the defect site was supported by the artificial esophagus without obvious stenosis. (2) There was no significant difference in body weight between groups A, B and C and group D at 4 weeks after operation (P > 0.05). (3) Hematoxylin-eosin staining demonstrated that in group A, granulation hyperplasia was seen at the esophageal defect site; a large number of inflammatory cells infiltrated; and the newly formed esophageal mucosa was partially covered with the patch tissue. In group B, the granulation tissue at the esophageal defect site had obvious proliferation, accompanied by infiltration of a large number of inflammatory cells. Inflammatory cell infiltration could be seen around the patch, and the esophageal mucosa was not covered with the patch tissue. In group C, granulation tissue proliferated at the esophageal defect site, and the infiltration of inflammatory cells was mild. Numerous new cells were seen in the artificial esophageal patch. The tissue structure of the original patch was unclear, and the esophageal mucosa completely covered the defect tissue. (4) Immunohistochemical staining exhibited that no actin α-positive cells were found in groups A and B, while blue-stained nuclei were seen, the original patch structure was loose, and actin α-specifically stained neovascular tissue was visible in group C. (5) It is concluded that the tea polyphenol-treated bovine pericardium decellularized tissue scaffold can be served as a good substitute for artificial esophagus.

Key words: artificial esophagus, esophagus rupture, tea polyphenol, bovine pericardium, decellularized tissue scaffold, cross-linking

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