中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (3): 404-408.doi: 10.3969/j.issn.2095-4344.0603

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

骨折模型小鼠周围组织E、P选择素含量变化及意义

罗  伟1，李翔翮1，杨先腾2，李森磊2，张  进2，田晓滨2，孙  立2   

  1.  (1贵州医科大学研究生院，贵州省贵阳市   550000；2贵州省人民医院骨科，贵州省贵阳市   550002)
  • 收稿日期:2018-07-21 出版日期:2019-01-28 发布日期:2019-01-28
  • 通讯作者: 孙立，博士，主任医师，教授，博士生导师，贵州省人民医院骨科，贵州省贵阳市 550002
  • 作者简介:罗伟，男，1991年生，四川省广安市人，汉族，贵州医科大学关节外科在读硕士，主要从事干细胞靶向迁移相关研究。
  • 基金资助:

    国家自然科学基金(81560356)，项目负责人：田晓滨；贵州省科学技术基金(黔科合J字[2015]2089号)，项目负责人：杨先腾

Alteration and significance of E-selectin and P-selectin in the tissues surrounding fracture in mouse models

Luo Wei1, Li Xianghe1, Yang Xianteng2, Li Senlei2, Zhang Jin2, Tian Xiaobin2, Sun Li2   

  1.  (1Graduate School of Guizhou Medical University, Guiyang 550000, Guizhou Province, China; 2Department of Orthopedics, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China)
  • Received:2018-07-21 Online:2019-01-28 Published:2019-01-28
  • Contact: Sun Li, MD, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China
  • About author:Luo Wei, Master candidate, Graduate School of Guizhou Medical University, Guiyang 550000, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81560356 (to TXB); the Science and Technology Foundation of Guizhou Province of China, No. [2015]2089 (to YXT)

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摘要：

文章快速阅读：

文题释义：
E、P选择素：是一类由糖基化单链跨膜蛋白构成的细胞黏附分子，主要分布于血小板及内皮细胞上，介导细胞间选择性识别、迁移、黏附，并在炎症反应、肿瘤转移及血栓形成等中起重要作用。
酶联免疫吸附法(ELISA)：是一类利用抗原抗体结合专一性进行的免疫反应，可定性、定量检测组织中的蛋白含量。ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。由于酶的催化效率很高，间接地放大了免疫反应的结果，使测定方法达到很高的灵敏度。
摘要
背景：E、P选择素是重要的黏附分子，参与机体内的炎症反应，而炎症反应对早期骨折的愈合具有积极的促进作用。
目的：探究小鼠骨折周围组织内E、P选择素含量变化及意义。
方法：取BALB/c小鼠36只，制作小鼠稳定胫骨骨折模型，建模后随机将小鼠分为1 h、6 h、12 h、24 h、48 h及3 d组，分别在对应时间点取骨折周围血肿肌肉组织，健侧同部位胫骨周围肌肉组织作为对照，采用酶联免疫吸附法对所取组织标本进行E、P选择素含量检测。
结果与结论：①骨折周围组织的E、P选择素含量均较健侧胫骨周围肌肉组织明显升高(P < 0.01)，其中E选择素含量在6 h左右达到高峰，P选择素含量在24 h左右达到峰值；②小鼠胫骨骨折后，骨折周围组织内E、P选择素含量明显上调，提示两者在小鼠骨折早期内可能介导了炎症细胞迁移、黏附，并参与了骨折后机体修复的病理过程。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-4095-3944(罗伟)

关键词: 小鼠, 骨折周围组织, 炎症反应, 黏附分子, 选择素, 酶联免疫吸附法, 组织构建

Abstract:

BACKGROUND: E-selectin and P-selectin are important adhesion molecules involved in the inflammatory response in vivo, and inflammatory response plays a positive role in promoting early fracture healing.
OBJECTIVE: To investigate the alterations and significance of E-selectin and P-selectin in the tissues surrounding fracture of mice.
METHODS: Thirty-six BALB/c mice were selected for establishing stable tibia fracture model. The mouse models were randomly divided into 1-, 6-, 12-, 24-, and 48-hour and 3-day groups. The hematoma tissue surrounding the fracture was removed at corresponding time, and contralateral tibial tissues at the healthy side were used as controls. The concentrations of E-selectin and P-selectin in tissues were detected by ELISA.
RESULTS AND CONCLUSION: The concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly higher than those in the healthy side (P < 0.01). E-selectin reached a peak at about 6 hours. P-selectin was the highest at about 24 hours. In summary, the concentrations of E-selectin and P-selectin in the tissues surrounding the fracture were significantly up-regulated, suggesting that E-selectin and P-selectin may mediate the migration and adhesion of inflammatory cells during the early stage of mouse fracture and play an important role in the pathological process of bone repair.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Fractures, Bone, E-Selectin, P-Selectin, Tissue Engineering

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