Changes of intestinal epithelial cells and their apoptotic factors Caspase-3, Bax and Bcl-2 under urinary environment

doi:10.3969/j.issn.2095-4344.3213

Abstract

Abstract: BACKGROUND: Reconstruction of the urinary tract with the ileum instead of the ureter has achieved a good clinical effect. There are yet no reports on what the adaptation relates to after the ileum acts as a substitute of the ureter. This study attempted to observe the effect of urine on the expression of apoptotic factors in intestinal epithelial cells, to further explore the possible underlying mechanism, and to provide experimental evidence for the pathological characteristics of the ileum instead of the ureter.
OBJECTIVE: To observe the effect of urine environment on the expression of Caspase-3, Bax and Bcl-2 in human intestinal epithelial cells and to further investigate whether the urine environment promotes the apoptosis of intestinal epithelial cells and its underlying mechanism.
METHODS: Human normal intestinal epithelial cells were treated with artificial urine that was diluted in culture medium at the rate of 0, 1:1, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, and 1:128 for 24, 48, and 72 hours. Cell counting kit-8 was used to observe the effect of urine on the proliferation of intestinal epithelial cells. RT-PCR and western blot assay were used to detect the mRNA and protein expressions of Caspase-3, Bax and Bcl-2 in intestinal epithelial cells.
RESULTS AND CONCLUSION: Caspase-3 and Bax were highly expressed in intestinal epithelial cells of the group with the highest urine concentration, and the expression level gradually decreased as the urine concentration gradient decreased. The expression of Bcl-2 in intestinal epithelial cells was low in the group with the highest urine concentration, and the expression level gradually increased as the urine concentration gradient decreased. The higher the urine concentration, the higher the expression of pro-apoptotic factors Caspase-3 and Bax in intestinal epithelial cells, and the lower the expression of anti-apoptotic factor Bcl-2,indicating that the urine can promote the apoptosis of intestinal epithelial cells.

Key words: urine, intestinal epithelial cells, apoptosis, ileum, ureter, Caspase-3, Bax, Bcl-2

CLC Number:

Xu Bin, Yang Xiushu, Liu Xuan, Wang Zhenxing. Changes of intestinal epithelial cells and their apoptotic factors Caspase-3, Bax and Bcl-2 under urinary environment[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(20): 3173-3177.

Figures/Tables 3

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