A porous silk fibroin scaffold is requried for repair of critical-size mandibular defects in rabbits

doi:10.3969/j.issn.2095-4344.2013.08.002

Abstract

Abstract:

BACKGROUND: Silk fibroin has excellent biocompatibility and biodegradability.
OBJECTIVE: To observe the effect of porous silk fibroin as a scaffold in repairing rabbit critical-size mandibular defects.
METHODS: The rabbit models of critical-size mandibular defects were established. Randomly selected one side of mandibular defects was filled with porous silk fibroin scaffold as experimental group, and the other side served as control group without disposal.
RESULTS AND CONCLUSION: (1) The gross specimen showed: After 12 weeks, in the experimental group, the surfaces of bone defect cavities were completely covered by new bone tissues, without materials prolapsed; in the blank control group, bone defect cavities were full of granulation tissues. (2) X-ray bone density: As time after surgery on, both experimental group and control group had higher bone density, and 12 weeks > 6 weeks > 2 weeks (P < 0.05), as well as, the bone density in the experimental group was higher than that in the control group at the same period (P < 0.05). (3) Hematoxylin-eosin staining of histopatological slices: At 12 weeks after surgery, in the experimental group, new bone and islands of trabecular bone increased significantly, and became thicker and denser. Materials were obviously loose inside, some areas collapsed. In the control group, there were scattered new bone tissues in edges of host bone, and no coarse trabecular bone formed. (4) Bone morphogenetic protein-2 immunohistochemical staining: As time on, the number of bone morphogenetic protein-2 positive cells in new bone trabeculae increased to varied degrees after surgery in both experimental group and control group, and the difference within group was statistically significant (P < 0.05); at each time, the number of bone morphogenetic protein-2-positive cells in the experimental group was higher than that in the control group (P < 0.05). These findings indicate that the porous silk fibroin scaffold has certain feasibility for in situ bone tissue engineering to repair bone defects.

Key words: biomaterials, tissue engineering bone materials, silk fibroin, scaffolds, critical-size bone defects, in situ tissue engineering, bone mineral density, bone morphogenetic protein, other grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Tang Ming, Zhao Xia, Chen Xin, Cui Xi-dong, Wen Jian-chuan, Gao Hai-he. A porous silk fibroin scaffold is requried for repair of critical-size mandibular defects in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1337-1343.

Figures/Tables 9

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