Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (16): 2554-2561.doi: 10.3969/j.issn.2095-4344.3118
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Chen Zhenyu, Zhang Xiaoning, Luo Yuxin, Liang Jianwei, Yan Chi
Received:
2020-06-28
Revised:
2020-07-02
Accepted:
2020-08-04
Online:
2021-06-08
Published:
2021-01-07
Contact:
Zhang Xiaoning, PhD, Associate Professor, State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
About author:
Chen Zhenyu, Master candidate, State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
Supported by:
CLC Number:
Chen Zhenyu, Zhang Xiaoning, Luo Yuxin, Liang Jianwei, Yan Chi. Evaluation of silk fibroin/curcumin composite film for promoting wound healing[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2554-2561.
2.5 丝素蛋白/姜黄素复合膜敷料创面疗效观察 2.5.1 创面愈合率 各组小鼠不同时间点创面大体观见图7。术后第3天,实验组创面与丝素蛋白/姜黄素复合膜敷料紧密贴合,创面处无渗出液积累,且创面已经开始缩小;对照组创面处还有少许渗出液且有出血现象,创面愈合率低于实验组(P < 0.05)。术后第7天,实验组创面明显缩小,伤口结痂;对照组创面开始缩小,创面愈合率低于实验组(P < 0.05)。术后第14天,实验组创面表面平滑而光亮,表明创面已基本愈合,但创面中心处仍有褐色印记;对照组创面中心仍有内陷,且内陷区域表面伴有少量结痂,创面愈合率低于实验组(P < 0.05)。两组不同时间点创面愈合率见图8。 "
实验发现,最初使用丝素蛋白/姜黄素复合膜敷料处理小鼠背部皮肤缺损创面时,丝素蛋白/姜黄素复合膜敷料紧紧贴合在创面上,随着小鼠皮肤缺损创面的逐渐愈合,丝素蛋白/姜黄素复合膜敷料也逐渐从创面脱落。 2.5.2 组织切片结果分析 图9为两组术后不同时间点的创面组织形态学观察。术后第3天,两组创面区域均有上皮化现象,均有肉芽组织形成,实验组再生上皮长度、肉芽组织厚大于对照组。术后第7天,两组再生上皮长度、肉芽组织厚度均增加,实验组的再生上皮长度、肉芽组织厚度仍大于对照组。术后第14天,两组创面区域均全部上皮化,实验组创面愈合区域皮下毛囊发育已经基本完成,可见大量胶原纤维及成纤维细胞整齐排列,为成熟的纤维结缔组织;对照组小鼠皮肤缺损创面附近未发现有纤维结缔组织的形,对照组小鼠背部创面愈合区域皮下毛囊则未全部长出,见图10。两组术后不同时间点的再生上皮长度、肉芽组织厚见图11。"
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