Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (20): 3117-3124.doi: 10.3969/j.issn.2095-4344.3199

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Combined effects of hyperbaric oxygen, vibration training and astaxanthin on bone mineral density, glucose metabolism and oxidative stress in diabetic osteoporosis rats

Liu Yulin1, Li Guotai2   

  1. 1Wisdom Health Care School, Chongqing City Management College, Chongqing 401331, China; 2College of Physical Education, Chongqing University, Chongqing 400044, China
  • Received:2020-02-22 Revised:2020-02-29 Accepted:2020-06-03 Online:2021-07-18 Published:2021-01-15
  • About author:Liu Yulin, Master, Lecturer, Wisdom Health Care School, Chongqing City Management College, Chongqing 401331, China

Abstract: BACKGROUND: Diabetic osteoporosis is a serious and common diabetic complication associated with a high disability and mortality rate that occurs in the skeletal system. There is no specific treatment plan for the treatment of diabetic osteoporosis, and a combination of hypoglycemic and anti-osteoporosis drugs is mainly used. Compared with single-drug therapy, combined therapy with non-drug adjuvants may have a more significant effect on patients’ bone mineral density.
OBJECTIVE: To explore the combined effects of hyperbaric oxygen + vibration training + astaxanthins on bone mineral density, bone metabolism, glucose metabolism, bone biomechanical performance, and oxidative stress in diabetic osteoporosis model rats.
METHODS: In this study, 80 rats were modeled with a DOP model, and another 10 rats were reared normally. Normally reared rats were listed as a control group. Diabetic osteoporosis rats were randomly divided into model control group, hyperoxic group (H group), vibration training group (V group), astaxanthin intake group (A group), hyperbaric oxygen + vibration training group (HV group), hyperbaric oxygen + astaxanthin group (HA group), vibration training + astaxanthin group (VA group), hyperbaric oxygen + vibration training + astaxanthin group (HVA group). Interventions in each group lasted for 16 weeks. Bone mineral density, glucose metabolism, and oxidative stress were tested at 16 weeks after intervention as well as at the end of the intervention. 
RESULTS AND CONCLUSION: After 16 weeks of intervention, the fasting plasma glucose, fasting serum insulin, and homeostasis model assessment insulin resistance indicators in the HV and HVA groups were significantly lower than those in the model control, H, V, A, HA, and VA groups (P < 0.05). Malondialdehyde content in the HVA group was significantly lower than that in the H, V, and HV groups (P < 0.05). The superoxide dismutase and glutathione peroxidase levels in the HVA group were significantly higher than those in the model control, H, V, and HV groups (P < 0.05). The bone mineral density in the HVA group was significantly higher than that in the model control, H, and A groups (P < 0.05). The parathyroid hormone and alkaline phosphatase levels in the HVA and HV groups were significantly lower than those in the H, V, A, HA, and VA groups (P < 0.05). The insulin-like growth factor 1 level was significantly higher in the HVA group than in the H, V, A, HA, and VA groups (P < 0.05). The maximum load, fracture load, and elastic model of the rat femur were significantly higher in the HV, HVA, V, and VA groups than in the model control, H, A, and HA groups 
(P < 0.05). To conclude, the combination of hyperbaric oxygen + vibration training + astaxanthin for diabetic osteoporosis as an adjuvant therapy can effectively control blood glucose, relieve insulin resistance, reduce bone resorption, increase bone mineral density, and improve bone biomechanical performance. Moreover, the combined effect is significantly better than that of hyperbaric oxygen or vibration training alone.

Key words: diabetic osteoporosis, hyperbaric oxygen, vibration training, astaxanthin, blood glucose, insulin resistance, bone mineral density, biomechanics

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