Effect of Bushen Jianpi Huoxue Recipe on transforming growth factor beta/bone morphogenetic protein 2 signaling pathway in the muscle and skeleton of ovariectomized rats

doi:10.3969/j.issn.2095-4344.3137

Abstract

Abstract: BACKGROUND: Bushen Jianpi Huoxue Recipe is an empirical prescription for treating osteoporosis in the Third Affiliated Hospital of Guangzhou University of Chinese Medicine. Studies have confirmed that the transforming growth factor β (TGF-β)/bone morphogenetic protein 2 (BMP-2) signaling pathway can regulate bone formation, which is an important pathway for the differentiation and synthesis of osteoblasts.
OBJECTIVE: To investigate the effect of Bushen Jianpi Huoxue Recipe on TGF-β/BMP-2 signaling pathway in the muscle and skeleton of ovariectomized rats.
METHODS: Seventy-two SPF female Sprague-Dawley rats aged 6 months were randomly divided into sham operation group (n=24) and model group (n=48). In the model group, ovariectomized models were made in rats. Twelve rats from each group were randomly selected 3 months after operation for testing bone density. The remaining 36 rats in the model group were randomly divided into model group, Bushen Jianpi Huoxue Recipe group (BSJPHX group, dose 2.979 g/kg), and alendronate sodium D3 tablets (II) group (ALN group, dose 1.02 mg/kg). The sham operation group and the model group were given an equal volume of normal saline. After 12 weeks, rats in each group were sacrificed under anesthesia. The bone density of lumbar vertebrae and femur were detected by dual-energy X-ray method. Real-time quantitative polymerase chain reaction (real-time PCR) and western blot were used to detect TGF-β and BMP-2 mRNA and protein in rat muscle and bone, respectively. The experiment was approved by the Ethics Committee of Guangzhou University of Chinese Medicine, with an approval No. S2016046.
RESULTS AND CONCLUSION: After 3 months of modeling, the bone mineral density of the model group was significantly lower than that of the sham operation group (P < 0.05). After 3 months of drug intervention, compared with the model group, bone mineral density was significantly increased in the BSJPHX group and ALN group, and TGF-β and BMP-2 mRNA and protein expression levels were significantly increased (P < 0.05). To conclude, Bushen Jianpi Huoxue Recipe regulates the transduction of TGF-β/BMP-2 signaling pathway by increasing the expression of TGF-β and BMP-2 at mRNA and protein levels, and then plays a preventive role in postmenopausal osteoporosis.

Key words: osteoporosis, bone mineral density, traditional Chinese medicine, Bushen Jianpi Huoxue Recipe, transforming growth factor β, bone morphogenetic protein 2, signaling pathway, rat

CLC Number:

Liu Zhijun, Liu Shaojin, Wei Hewei, Wan Lei, Huang Hongxing, Qiao Rongqin. Effect of Bushen Jianpi Huoxue Recipe on transforming growth factor beta/bone morphogenetic protein 2 signaling pathway in the muscle and skeleton of ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(14): 2219-2223.

Figures/Tables 5

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