中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (32): 5085-5089.doi: 10.3969/j.issn.2095-4344.2014.32.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

Spry1在miR-21调控人骨髓间充质干细胞成骨分化中的作用

杨  楠1,周  威1,王  光2,丁  寅3,金  岩4   

  1. 1解放军第309医院口腔科,北京市  100091;2解放军91551部队门诊部,江西省九江市  332005;3解放军第四军医大学口腔医院正畸科,陕西省西安市  710032;4解放军第四军医大学组织工程中心,陕西省西安市  710032
  • 收稿日期:2014-06-26 出版日期:2014-08-06 发布日期:2014-09-18
  • 通讯作者: 丁寅,博士生导师,主任医师,解放军第四军医大学口腔医院正畸科,陕西省西安市 710032 并列通讯作者:金岩,博士生导师,教授,解放军第四军医大学组织工程中心,陕西省西安市 710032
  • 作者简介:杨楠,女,1981年生,北京市人,满族,2012年解放军第四军医大学毕业,博士,主治医师,主要从事口腔正畸及干细胞研究。
  • 基金资助:

    国家重点基础研究发展计划(2011CB964700)

Role of Spry1 in osteogenic differentiation of human bone marrow mesenchymal stem cells under miR-21 regulation

Yang Nan1, Zhou Wei1, Wang Guang2, Ding Yin3, Jin Yan4   

  1. 1Department of Stomatology, the 309 Hospital of PLA, Beijing 100091, China; 2Outpatient Department of Troop 91551, Jiujiang 332005, Jiangxi Province, China; 3Department of Orthodontics, Stomatological Hospital of Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China; 4Center of Tissue Engineering, Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China
  • Received:2014-06-26 Online:2014-08-06 Published:2014-09-18
  • Contact: Ding Yin, Doctoral supervisor, Chief physician, Department of Orthodontics, Stomatological Hospital of Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China Corresponding author: Jin Yan, Doctoral supervisor, Professor, Center of Tissue Engineering, Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China
  • About author:Yang Nan, M.D., Attending physician, Department of Stomatology, the 309 Hospital of PLA, Beijing 100091, China
  • Supported by:

    The National Basic Research Program of China (973 Program), No. 2011CB964700

摘要:

背景:前期研究发现,miR-21在骨髓间充质干细胞成骨分化中表达上升,但是miR-21在骨髓间充质干细胞成骨分化中的作用及分子机制尚不明确。
目的:验证miR-21的靶基因Spry1,探明Spry1在人骨髓间充质干细胞成骨分化中的作用。
方法:荧光素酶报告验证miR-21靶基因Spry1,Western Blot检测Spry1在人骨髓间充质干细胞成骨过程的表达。构建Spry1慢病毒表达载体,感染人骨髓间充质干细胞。碱性磷酸酶、茜素红染色、RT-PCR和Western Blot分析Spry1高表达后人骨髓间充质干细胞成骨能力的改变。
结果与结论:荧光素酶报告提示Spry1为miR-21的靶基因,在人骨髓间充质干细胞成骨过程中表达量下降。上调Spry1能够抑制人骨髓间充质干细胞的成骨分化。结果表明Spry1作为miR-21的靶基因负向调控人骨髓间充质干细胞的成骨分化,在骨形成过程发挥着重要作用。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

关键词: 干细胞, 骨髓干细胞, 骨髓间充质干细胞, miR-21, Spry1, 成骨分化, 973项目

Abstract:

BACKGROUND: Previous studies have found that miR-21 expression is increased during osteogenic differentiation of bone marrow mesenchymal stem cells, but the action and molecular mechanism of miR-21 are still unclear.
OBJECTIVE: To verify the target gene of miR-21, Spry1, and to explore the role of Spry1 in osteogenic differentiation of human bone marrow mesenchymal stem cells.
METHODS: Luciferase report was used to verify Spry1 gene targeted by miR-21, and western blot assay was used to detect the expression of Spry1 in the osteogenesis of human bone marrow mesenchymal stem cells. Spry1 expression vector was established and transfected into human bone marrow mesenchymal stem cells. Osteogenesis ability of human bone marrow mesenchymal stem cells was analyzed after Spry1 high expression by alkaline phosphatase, alizarin red staining, RT-PCR and western blot.
RESULTS AND CONCLUSION: Luciferase report suggested that Spry1 was a target gene of miR-21. The expression level of Spry1 was decreased in the osteogenesis of human bone marrow mesenchymal stem cells. Increasing expression of Spry1 could inhibit osteogenic differentiation of human bone marrow mesenchymal stem cells. These results indicate that Spry1 as a target gene of miR-21 negatively regulates osteogenic differentiation 
of human bone marrow mesenchymal stem cells, and plays an important role in bone formation process.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

Key words: bone marrow, mesenchymal stem cells, cell transdifferentiation, osteoblasts, microRNAs, gene expression

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