中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (24): 3773-3778.doi: 10.3969/j.issn.2095-4344.2014.24.001

• 骨组织构建 bone tissue construction •    下一篇

Micro CT定量研究去卵巢山羊胫骨平台松质骨微结构特点

王德志,陈世昌,梁正洋,王  友   

  1. 上海交通大学医学院附属第九人民医院骨科,上海市  200011
  • 修回日期:2014-04-26 出版日期:2014-06-11 发布日期:2014-06-11
  • 通讯作者: 王友,博士,教授,主任医师,博士生导师,上海交通大学医学院附属第九人民医院骨科,上海市 200011
  • 作者简介:王德志,男,1988年生,山东省日照市人,汉族,上海交通大学医学院附属第九人民医院骨科在读硕士,主要从事膝关节领域的研究工作。
  • 基金资助:

    国家科技支撑计划课题(2012BAI18B07)

Micro CT study on trabecular microstructure of tibial plateau in ovariectomized goats

Wang De-zhi, Chen Shi-chang, Liang Zheng-yang, Wang You   

  1. Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200011, China
  • Revised:2014-04-26 Online:2014-06-11 Published:2014-06-11
  • Contact: Wang You, M.D., Professor, Chief physician, Doctoral supervisor, Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200011, China
  • About author:Wang De-zhi, Studying for master’s degree, Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200011, China
  • Supported by:

    the National Science & Technology Pillar Program of China, No. 2012BAI18B07

摘要:

背景:骨质疏松时胫骨平台松质骨微结构发生显著变化,Micro CT是一种能够全面、立体、无创测量骨微结构、评估骨质量及预测骨强度的新兴技术,近年来在骨质疏松研究领域得到日益广泛的应用。
目的:应用Micro CT技术定量研究去卵巢山羊胫骨平台松质骨的微结构特点。
方法:将12只2.5岁健康雌性山羊随机分为去卵巢组和假手术组,去卵巢组行卵巢切除,假手术组切除等量腹腔脂肪组织,每组各6只。两组实验动物分别在术后2,4年处死,分离并截取左侧胫骨平台,行Micro CT扫描,分别测量胫骨平台骨骺松质骨和干骺端松质骨微结构参数。
结果与结论:术后2和4年,与假手术组相比,去卵巢组胫骨平台骨骺和干骺松质骨微观结构参数-骨体积分数、骨小梁数量和骨小梁厚度均降低(P < 0.05),骨小梁分离度均升高(P < 0.05),基本呈时间依赖性变化。仅在术后4年,去卵巢组骨骺松质骨微观结构参数骨小梁厚度与假手术组相比差异无显著性意义(P > 0.05),其骨小梁分离度、骨小梁厚度与去卵巢组术后2年相比差异无显著性意义(P > 0.05)。无论术后2或4年,与假手术组相比,去卵巢组干骺端松质骨微结构参数的改变均比骨骺松质骨明显。结果证实,山羊胫骨平台骨骺松质骨微结构参数与干骺端松质骨具有一定的差异;骨质疏松时山羊胫骨平台松质骨微结构改变呈现出区域性特点,干骺端松质骨较骨骺松质骨微结构退变更为显著。



中国组织工程研究
杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


全文链接:

关键词: 组织构建, 骨组织构建, 软骨组织构建, Micro CT, 微结构, 松质骨, 胫骨平台, 骨质疏松, 山羊, 骨密度, 绝经, 模型

Abstract:

BACKGROUND: Trabecular microstructure of tibial plateau changes significantly under the progress of osteoporosis. Micro CT is a comprehensive, three-dimensional, noninvasive technology, which provides new method of measuring bone microstructure, evaluating bone quality and predicting bone strength. In recent years, this technique has been widely used in the field of osteoporosis study.
OBJECTIVE: To investigate the trabecular microstructure of tibial plateau in ovariectomized goats by Micro CT.
METHODS: Twelve female goats at 2.5 years old were randomly divided into ovariectomy (OVX) and sham groups, and each group included six goats. Goats in OVX group underwent ovarian surgery, while sham-operated goats removed equal amount of abdominal fat tissue. Half of goats in both OVX and sham groups were executed at 2 and 4 years after surgery, respectively. The left tibial plateau was harvested and a high resolution Micro CT was used to identify the trabecular microstructure parameters of metaphysis and epiphysis.
RESULTS AND CONCLUSION: Compared with the sham-operated goats at 2 and 4 years post-surgery, bone volume fraction, trabecular number and trabecular thickness were decreased in the OVX group (P < 0.05), while trabecular separation was increased (P < 0.05), which all appeared a time-dependent manner. As exceptions, for the comparison of trabecular thickness in epiphysis between OVX group and sham group at 4 years post-surgery  (P > 0.05), and for the comparison of both trabecular thickness and trabecular separation in epiphysis at 2 years post-surgery (P > 0.05), there was no significant difference. At 2 and 4 years after surgery, microstructure parameters in metaphysis changed more obviously than them in epiphysis. The trabecular microstructure showed the differences between metaphysis and epiphysis of tibial plateau. The change of trabecular microstructure appears to be site-specific in the progress of osteoporosis in tibial plateau, and the microstructure deterioration of trabecular bone in the metaphysis of tibial plateau happens severer than that in the epiphysis.



中国组织工程研究
杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


全文链接:

Key words: osteoporosis, ovariectomy, tibia, microstructure, bone mineral density

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