中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3751-3754.doi: 10.3969/j.issn.1673-8225.2012.20.032

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

银杏叶提取物对胚胎大鼠中脑原代细胞及多巴胺能神经元活性的影响*★

奚淑芳1，王群江2，朱灿宏1，胡  蓉1，端礼荣3   

  1. 1镇江市第一人民医院，江苏省镇江市 212002；2浙江省人民医院，浙江省杭州市 310051；3江苏大学医学院预防医学系，江苏省镇江市  212001
  • 收稿日期:2011-09-07 修回日期:2011-11-11 出版日期:2012-05-13 发布日期:2012-05-13
  • 作者简介:奚淑芳★，女，1976年生，陕西省蒲城市人，汉族，2005年解放军第四军医大学毕业，硕士，主要从事帕金森病及老年痴呆的研究。xishuf@163.com 并列第一作者：王群江★，男，1976年生，新疆维吾尔族自治区精河县人，汉族，2007年浙江中医药大学毕业，硕士，主要从事脑血管疾病研究。
  • 基金资助:

    2006年江苏省镇江市科技计划项目基金资助(SH2006043)，项目名称：银杏叶提取物对大鼠中脑原代细胞的作用。

Effect of ginkgo biloba extract on activities of primary cells from embryonic rat mesencephalon and dopaminergic neurons  

Xi Shu-fang1, Wang Qun-jiang2, Zhu Can-hong1, Hu Rong1, Duan Li-rong3   

  1. 1First People’s Hospital of Zhenjiang, Zhenjiang  212002, Jiangsu Province, China; 2Zhejiang Provincial People’s Hospital, Hangzhou  310051, Zhejiang Province, China; 3Department of Preventive Medicine, Medical College of Jiangsu University, Zhenjiang  212001, Jiangsu Province, China
  • Received:2011-09-07 Revised:2011-11-11 Online:2012-05-13 Published:2012-05-13
  • About author:Xi Shu-fang★, Master, First People’s Hospital of Zhenjiang, Zhenjiang 212002, Jiangsu Province, China xishuf@163.com Wang Qun-jang, Master, Zhejiang Provincial People’s Hospital, Hangzhou 310051, Zhejiang Province, China
  • Supported by:

    Science and Technology Project of Zhenjiang City in 2006, No. SH2006043*

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摘要：

背景：银杏叶提取物具有清除自由基和过氧化脂质，对抗兴奋性神经递质释放等多种药理活性。
目的：观察银杏叶提取物EGB761对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinum, MPP+)及左旋精氨酸(L-arginine, L-Arg)干预后的中脑原代细胞及多巴胺能神经元是否具有保护作用。
方法：分离培养SD胚胎大鼠中脑神经细胞，分组培养：空白对照组、MPP+组、L-Arg组、MPP++L-Arg组、MPP++EGB761组、MPP++EGB761+L-Arg组。
结果与结论：①细胞A值：MPP+组、MPP++L-Arg组显著低于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P < 0.05)，MPP++L-Arg组低于MPP+组(P < 0.05)，MPP++EGB761+L-Arg组低于MPP++EGB761组(P < 0.05)。②酪氨酸羟化酶阳性细胞数：MPP+组、MPP++L-Arg组显著低于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P < 0.05)，MPP++L-Arg组低于MPP+组(P < 0.05)，MPP++EGB761+L-Arg组低于MPP++EGB761组(P < 0.05)。③细胞一氧化氮水平：MPP+组、MPP++L-Arg组显著高于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P < 0.05)，MPP++L-Arg组高于MPP+组(P < 0.05)，MPP++EGB761+L-Arg组高于MPP++EGB761组(P < 0.05)。说明L-Arg可加重MPP+对多巴胺能神经元的损伤作用，EGB761对损伤后的胚胎大鼠中脑原代培养细胞有保护作用，此作用可能是通过抑制诱导型一氧化氮合酶活性降低一氧化氮产生完成的。
关键词：银杏叶提取物；一氧化氮；左旋精氨酸；1-甲基-4-苯基吡啶离子； 神经细胞 
doi:10.3969/j.issn.1673-8225.2012.20.032

关键词: 银杏叶提取物, 一氧化氮, 左旋精氨酸, 1-甲基-4-苯基吡啶离子, 神经细胞 

Abstract:

BACKGROUND: Ginkgo biloba extract (EGB) has the pharmacological activities of free radical and lipid peroxidation scavenging and release of excitatory neurotransmitter resisting.
OBJECTIVE: To explore whether EGB761 has protective effect on primary cells from embryonic rat mesencephalon and dopaminergic neurons from injuries induced by 1-methyl-4-phenylpyridinum (MPP+) and L-arginine (L-Arg).  
METHODS: Midbrain nerve cells from Sprague Dawley embryonic rats were isolated and cultured. The cells were divided into control group, MPP+ group, L-Arg group, MPP++L-Arg group, MPP++EGB761 group and MPP++EGB761+L-Arg group.
RESULTS AND CONCLUSION: Comparison results of cell A values and numbers of tyrosine hydroxylase-positive cells among the groups: these indexes in the MPP+ group and MPP++L-Arg group were significantly decreased compared with the control group, L-Arg group, MPP++EGB761 group and MPP++EGB761+L-Arg group (P < 0.05), those of the MPP++L-Arg group were lower compared with the MPP+group (P < 0.05), and those of the MPP++EGB761+L-Arg group were lower than the MPP++ EGB761 group (P < 0.05). Cell nitric oxide levels: the levels in the MPP+ group and MPP++L-Arg group were obviously higher compared with the control group, L-Arg group, MPP++EGB761 group and MPP++EGB761 L-Arg group (P < 0.05), the level of the MPP++L-Arg group was higher compared with the MPP+ group (P < 0.05), and that of the MPP++EGB761+L-Arg group was higher than the MPP++EGB761 group (P < 0.05). It is indicated that L-Arg can increase the MPP+-induced damage to nopaminergic neurons, and EGB761 has the protective effect on injured primary cells from embryonic rat mesencephalon which may derived from reduction of nitric oxide by inhibiting the activity of inducible nitric oxide synthase.

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