中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7121-7125.doi: 10.3969/j.issn.2095-4344.2012.38.019

• 药物控释材料 drug delivery materials • 上一篇    下一篇

载盐酸四环素壳聚糖缓释膜的制备

丁 曼1，王华林1，姜绍通2   

  1. 1合肥工业大学化学工程学院，安徽省合肥市 230009
    2合肥工业大学生物与食品工程学院，安徽省合肥市 230009
  • 收稿日期:2011-12-31 修回日期:2012-02-20 出版日期:2012-09-16 发布日期:2012-09-16
  • 通讯作者: 王华林，教授，合肥工业大学化学工程学院，安徽省合肥市 230009 doc_hlwang@ hfut.edu.cn
  • 作者简介:丁曼★，女，1987年生，安徽省阜阳市人，汉族，合肥工业大学在读硕士，主要从事天然高分子材料的研究。 doc_dingman870813@163.com

Preparation of tetracycline hydrochloride-loaded chitosan controlled-released films

Ding Man1, Wang Hua-lin1, Jiang Shao-tong2   

  1. 1School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China
    2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China
  • Received:2011-12-31 Revised:2012-02-20 Online:2012-09-16 Published:2012-09-16
  • Contact: Wang Hua-lin, Professor, School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China doc_hlwang@ hfut.edu.cn
  • About author:Ding Man★, Studying for master’s degree, School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China doc_dingman870813@163.com

PDF

437

被引次数

4

摘要：

背景：壳聚糖具有良好的生物相容性、生物可降解性及较好的抗菌活性。
目的：使用流延法制备载有不同盐酸四环素的壳聚糖载药纳米纤维膜，观察其缓释性能和抑菌性能。
方法：采用流延法制备厚度为0.03 mm的载有不同含量(0，3%，5%，10%，20%)盐酸四环素的壳聚糖载药缓释膜，测定载药率，绘制盐酸四环素缓释曲线。分别用液体培养和固体培养检测载药缓释膜的体外抑菌性能，用磷酸盐缓冲液观察载药缓释膜的降解性能。
结果与结论：随盐酸四环素含量的增加，缓释膜载药率降低，突释量增大。载药壳聚糖膜可有效抑制金黄色葡萄球菌的生长，并随盐酸四环素含量的增加，抑菌效果提高，当盐酸四环素含量超过10%时，载药壳聚糖膜抑菌率的变化不明显。盐酸四环素的加入加快了壳聚糖膜降解，并随着盐酸四环素含量的增加，降解速率增大，当盐酸四环素载药量超过10%时，降解可在8 d内完成。相比较得出，盐酸四环素含量在10%时，在疗效和性价比上是较好的选择。

关键词: 盐酸四环素, 壳聚糖, 缓释, 抑菌, 生物材料

Abstract:

BACKGROUND: Chitosan has good biocompatibility, biodegradability and antibiotic activity.
OBJECTIVE: To prepare chitosan nanofiber films with various tetracycline hydrochloride contents by using tape casting method, and to investigate their performance in preventing bacterial infections as well as their controlled-release characteristic.
METHODS: Chitosan/tetracycline hydrochloride controlled-release films, 0.03 mm in thickness, with various tetracycline hydrochloride contents (0, 3%, 5%, 10%, 20%) were prepared by tape casting method. Drug loaded efficiency was determined, and drug release profiles were drawn. The in vitro antibiotic activity of drug release films was tested by using solution culture and solid culture methods. Degradation of the films was observed by phosphate buffer solution method.
RESULTS AND CONCLUSION: Drug loaded efficiencies of the chitosan/tetracycline hydrochloride films were decreased with the tetracycline hydrochloride contents increased. Besides, burst release amount was increased. The chitosan/tetracycline hydrochloride films could inhibit staphylococcus aureus growth, and with the increase of the tetracycline hydrochloride contents, their antibacterial effect was enhanced. Until the tetracycline hydrochloride content was over 10%, the antibacterial effect did not change significantly. Tetracycline hydrochloride could promote the degradation of chitosan films, and with the increased tetracycline hydrochloride contents, the rate of degradation was faster. Chitosan/tetracycline hydrochloride films with more than 10% tetracycline hydrochloride could be degraded completely within 8 days. These findings suggest that the chitosan film with over 10% tetracycline hydrochloride is a better choice considering the price and curative effect.

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