中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 2033-2038.doi: R318

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

氧化应激和Rac1/2对静脉壁的影响及在创伤性深静脉血栓形成中的作用******☆

李兴国1，郑宏宇1，李  文2，李宏昆1，赵学凌1，王  兵1，吴雪梅1   

  1. 1昆明医学院第一附属医院骨科，云南省昆明市  650032；2云南省人民医院心内科，云南省昆明市  650032
  • 收稿日期:2011-09-12 修回日期:2011-12-12 出版日期:2012-03-11 发布日期:2012-03-11
  • 通讯作者: 吴雪梅，主管护师，昆明医学院第一附属医院骨科，云南省昆明市 650032
  • 作者简介:李兴国☆，男，1981年生，云南省楚雄人，汉族，昆明医学院在读骨科博士，讲师，主要从事深静脉血栓形成分子机制的研究。 并列第一作者：郑宏宇，男，1978年生，云南省大理人，汉族，昆明医学院在读博士，医师，主要从事深静脉血栓预测诊断的研究。
  • 基金资助:

    国家自然科学基金资助项目(30960389，81060151，81160236)；云南省科技厅-昆明医学院联合项目(2009cd159)；云南省重点新产品项目开发计划项目(2010BC010)昆明医学院博士研究生创新基金资助项目(2011D07)。

Effects of oxidative stress and Rac1/2 on venous wall and their roles in traumatic deep vein thrombosis******☆

Li Xing-guo1, Zheng Hong-yu1, Li Wen2, Li Hong-kun1, Zhao Xue-ling1, Wang Bing1, Wu Xue-mei1   

  1. 1Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China; 2Department of Cardiology, Yunnan People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2011-09-12 Revised:2011-12-12 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Wang Bing, Doctor, Associate chief physician, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China wangbingdoctor@126.com
  • About author:Li Xing-guo☆, Studying for doctorate, Lecturer, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China ynlxg1981@126.com Zheng Hong-yu, Studying for doctorate, Physician, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China zhenghongyu@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*, 81060151*, 81160236*; the Joint Foundation of Yunnan Science and Technology Department and Kunming Medical University, No. 2009cd159*; Yunnan Key New Product Development Projects, No. 2010BC010*; the Innovation Foundation for Doctors in Kunming Medical University, No. 2011D07*

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摘要：

背景：深静脉血栓形成的分子机制及核心调控网络目前仍未完全阐明。
目的：观察氧化应激和Rac1/2在大鼠创伤性深静脉血栓形成中的作用。
方法：将SD大鼠采用股静脉钳夹联合下肢石膏制动构建大鼠深静脉血栓模型。不同时间点(造模后2.5 h和25 h)解剖股静脉观测血栓的发生率及严重程度。再将模型大鼠分为：血栓形成前组(造模后2.5 h)、血栓形成组(创伤后25 h)、血栓未形成组(造模后    25 h)。获取股静脉壁组织，提取总蛋白质和总RNA。
结果与结论：比色法检测结果显示，相比血栓未形成组，血栓形成组大鼠股静脉组织中丙二醛的含量最高(P < 0.05)，血栓形成前组次之(P < 0.05)；而总超氧化物岐化酶、谷胱甘肽还原酶的活性在血栓形成组最低，血栓形成前组次之(P < 0.05)。基因芯片分析及real-time PCR结果发现，相比血栓未形成组，血栓形成组大鼠股静脉组织中Rac1和Rac2的表达最高(P < 0.05)，血栓形成前组次之(P < 0.05)。结果证实，局部静脉血管壁组织中丙二醛，Rac1/2表达上调，总超氧化物岐化酶和谷胱甘肽还原酶活性降低，可能导致创伤性深静脉血栓的形成。
B 

关键词: 氧化应激, 丙二醛, 总超氧化物岐化酶, 谷胱甘肽还原酶, Rac1/2, 深静脉血栓, 组织构建

Abstract:

BACKGROUND: The molecular mechanism and core regulatory network of deep vein thrombosis are not fully clarified yet.
OBJECTIVE: To explore the roles of oxidative stress and Rac1/2 in rat deep vein thrombosis.
METHODS: Deep vein thrombosis model in SD rats was established by a champing method femoral veins clamping combined with fixation of the lower extremity with plaster. The incidence and serious degree of thrombus were observed by dissecting rat femoral vein at different time points (2.5 and 25 hours after modeling). The model rats were divided into pre-thrombogenesis group (2.5 hours after modeling), thrombogenesis group (25 hours after modeling) and non-thrombogenesis group (25 hours after modeling). Then total RNA and protein were extracted from the femoral venous wall tissues.
RESULTS AND CONCLUSION: Colorimetry results showed that compared with the non-thrombogenesis group, the concentration of malondiadehyde in rat femoral vein wall tissues of the thrombogenesis group was the highest (P < 0.05), followed by that of the pre-thrombogenesis group (P < 0.05). The concentrations of total superoxide dismutase and glutathione reductase in the thrombogenesis group were the lowest, followed by those in the pre-thrombogenesis group (P < 0.05). The results of gene chip hybridization analysis and real-time PCR showed that compared with the non-thrombogenesis group, the expressions of Rac1 and Rac2 in rat femoral vein wall tissues of thrombogenesis group increased the most, followed by that of the pre-thrombogenesis group (P < 0.05). These findings indicate that the up-regulation of malondialdehyde and Rac1/2 as well as the activity decrease of total superoxide dismutase and glutathione reductase may lead to the formation of deep venous thrombosis.

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