中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 813-817.doi: 10.3969/j.issn.1673-8225.2012.05.013

• 肾移植 kidney transplantation • 上一篇    下一篇

无心跳供肾模型大鼠的安全热缺血时限*★

戴  晨，李素华，刘  健   

  1. 新疆医科大学第一附属医院肾病科，新疆维吾尔自治区乌鲁木齐市     830000
  • 收稿日期:2011-08-20 修回日期:2011-10-13 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 刘健，教授，主任医师，硕士生导师，新疆医科大学第一附属医院肾病科，新疆维吾尔自治区乌鲁木齐市 830000 jianliu@medmail.com
  • 作者简介:戴晨★,女，1986年生，新疆维吾尔自治区乌鲁木齐市人，汉族，新疆医科大学在读硕士，主要从事肾脏保存液方面的研究。 mimose1986@sina.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金(2011211A058)，项目名称：肾移植早期体内不同免疫状态下T细胞亚群、TH1/TH2细胞因子和MICA抗体变化的前瞻研究。

Safe duration limits of tolerance to warm ischemia of kidney grafts from non-heart-beating donor rats

Dai Chen, Li Su-hua, Liu Jian   

  1. Department of Nephrology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-08-20 Revised:2011-10-13 Online:2012-01-29 Published:2012-01-29
  • Contact: Liu Jian, Professor, Chief physician, Master’s supervisor, Department of Nephrology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China jianliu@medmail.com
  • About author:Dai Chen★, Studying for master’s degree, Department of Nephrology, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China mimose1986@sina.com
  • Supported by:

     the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2011211A058*

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摘要：

背景：近年来心脏停博供体已然成为最有潜力的移植供体来源。目前有关于供肾安全热缺血期的时限研究各报道差异较大，更未上升到安全时限的理论高度。
目的：探讨建立大鼠无心跳供肾模型的实验方法，并分析此供体耐受热缺血的安全时限。
方法：采用断延髓法建立大鼠无心跳供肾模型，并按肾移植供体经历的热缺血时间0，10，30，60 min将其分为4组。观察离体肾脏经过不同热缺血期后，供肾的组织病理学及肾组织丙二醛含量变化。
结果与结论：光镜下观察，热缺血30 min以前，肾脏病理改变为可逆性改变，随着热缺血时间的延长病理改变向着不可逆方向发展。与无热缺血期比较，热缺血10 min组、30 min组及60 min组的丙二醛含量均有所升高(P < 0.05)；与热缺血10 min组比较，热缺血30 min组及60 min组丙二醛含量明显升高(P < 0.05)；但热缺血30 min与60 min组差异无显著性意义(P > 0.05)。提示可成功采用断延髓法建立无心跳供肾模型，并在此基础上，结合供肾的病理结果和肾组织丙二醛含量变化，综合评定无心跳供肾所能耐受的安全热缺血时限为30 min。

关键词: 心脏停搏供体, 肾移植, 热缺血, 安全时限, 大鼠

Abstract:

BACKGROUND: Recently, non-heart-beating donor has become the most potential source of transplant donors. At present, the research reports on safe duration limits of tolerance to warm ischemia of kidney grafts differ significantly, much less reach the theoretical perspective of safe duration limits.
OBJECTIVE: To investigate the establishment of kidney transplantation model of non-heart-beating donors in rats and to explore the safe duration limits of tolerance to warm ischemia of the donor.
METHODS: Rat medulla oblongata was broken to establish the kidney transplantation model of non-heart-beating donors in rats. The rats were divided into four groups according to the warm ischemia time (0, 10, 30 and 60 minutes). Changes of histopathology and malondialdehyde content in kidney off-body with different warm ischemia time were observed. 
RESULTS AND CONCLUSION: Under light-microscopy, renal pathological changes were reversible within 30 minutes of warm ischemia. Along with the extension of warm ischemia time, pathological changes developed towards irreversible direction. The malondialdehyde contents in the groups of 10, 30 and 60 minutes warm ischemia were increased than that in the group without warm ischemia (P < 0.05). The malondialdehyde contents in the groups of 30 and 60 minutes warm ischemia were significantly increased than that in the 10 minutes warm ischemia group (P < 0.05). There was no significant difference in malondialdehyde content between the groups of 30 and 60 minutes warm ischemia (P > 0.05). These findings indicate that the kidney transplantation model of non-heart-beating donors was established successfully by breaking the medulla oblongata. On that basis, the safe duration limits of tolerance to warm ischemia of kidney grafts from non-heart-beating donor is determined as 30 minutes according to the pathological results and malondialdehyde content changes in donor kidneys.

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