中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9587-9592.doi: 10.3969/j.issn.1673-8225.2011.51.021

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

Fe3O4纳米粒子的细胞相容性

龚君佐1，屠重棋2，段  宏2，周绍兵3   

  1. 1川北医学院急诊医学系，四川省南充市   637000
    2四川大学华西医学中心骨科，四川省成都市   610046
    3西南交通大学材料先进技术教育部重点实验室，四川省成都市   610046
  • 收稿日期:2011-06-17 修回日期:2011-08-25 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 屠重棋，博士，博士导师，教授，四川大学华西医学中心骨科，四川省成都市 610046 tuchongqi@ 163.com
  • 作者简介:龚君佐★，男，1975年生，四川省南充市人，汉族，2008年四川大学华西医学中心毕业，硕士，讲师，主要从事骨肿瘤骨病方面的研究。 gongjunzuo@ 163.com

Cellular compatibility of Fe3O4 nanoparticles

Gong Jun-zuo1, Tu Zhong-qi2, Duan Hong2, Zhou Shao-bing3   

  1. 1Department of Emergence Medicine, North Sichuan Medical College, Nanchong  637000, Sichuan Province, China
    2Department of Orthopedics, West China Center of Medical Sciences, Sichuan University, Chengdu  610046, Sichuan Province, China
    3Key Laboratory of Advanced Material Technology, the Ministry of Education, Southwest Jiaotong University, Chengdu  610046, Sichuan Province, China
  • Received:2011-06-17 Revised:2011-08-25 Online:2011-12-17 Published:2011-12-17
  • Contact: Tu Zhong-qi, Doctor, Doctoral supervisor, Professor, Department of Orthopedics, West China Center of Medical Sciences, Sichuan University, Chengdu 610046, Sichuan Province, China tuchongqi@163.com
  • About author:Gong Jun-zuo★, Master, Lecturer, Department of Emergence Medicine, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China gongjunzuo@ 163.com

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摘要：

背景：无论是作为化疗药物载体还是作为肿瘤热疗介质，Fe3O4纳米粒子与肿瘤细胞微观结构的作用都有待于深入研究。
目的：分析Fe3O4纳米粒子的细胞相容性，探讨其在骨肉瘤化疗中作为药物载体的应用和存在的问题。
方法：参照GB/T16886.5-2003 (医疗器械生物学评价 第5部分：体外细胞毒性试验)的评价标准和要求，偶联十六烷基三甲基溴化铵、聚乙二醇、油酸钠Fe3O4纳米粒子胶体溶液，分别与大鼠原发骨肉瘤细胞和人成纤维细胞共培养的方式对其进行细胞毒性测试。
结果及结论：偶联油酸钠Fe3O4纳米粒子细胞相容性良好，参照GB/T16886.5-2003标准属于安全范围。偶联十六烷基三甲基溴化铵Fe3O4纳米粒子细胞相容性差，不适宜作为化疗药物载体进入人体。偶联聚乙二醇Fe3O4纳米粒子有一定的细胞相容性，作为磁靶向热化疗载体还需研究。

关键词: Fe3O4纳米粒子, 细胞相容性, 细胞毒性, 药物载体, 化疗

Abstract:

BACKGROUND: Whether as chemotherapy or as a medium of thermotherapy in malignant tumor, the effect and microstructure of Fe3O4 nanoparticles need further studies.
OBJECTIVE: To analyze the cellular compatibility of Fe3O4 nanoparticles and to investigate the application and problems of Fe3O4 nanoparticles as drug carriers in the chemotherapy of malignant bone tumors.
METHODS: According to the evaluate criteria and requirement of GB/T 16886. 5-2003 (Part V: cytotoxicity test in vitro, Biological Evaluation for Medical Instrument), the colloidal solution of Fe3O4 nanoparticles conjugated with cetrimonium bromide or polyethylene glycol or sodium oleate was co-cultured with osteosarcoma UMR-16 cells and human fibroblast cells, respectively, hence to assess the cytotoxicity characteristics.
RESULTS AND CONCLUSION: According the criteria of GB/T16886.5-2003, Fe3O4 nanoparticles conjugated with sodium oleate had good cellular compatibility, whereas, the cellular compatibility of Fe3O4nanoparticles conjugated with cetrimonium bromide was poor, which were not considered as a drug carrier of chemotherapy drug using in clinical practice. Fe3O4 nanoparticles conjugated with polyethylene glycol had a certain cellular compatibility, which needed further studies used as drug carriers.

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