中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9593-9597.doi: 10.3969/j.issn.1673-8225.2011.51.022

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

纯钛种植体表面微弧氧化涂层的生物相容性

张  强1，王健平2，胡美玲3，孟祥才4，王  静4   

  1. 1长治市第二人民医院修复正畸科，山西省长治市  046000
    2佳木斯大学附属口腔医院牙体牙髓病一科，黑龙江省佳木斯市  154002
    3哈尔滨医科大学附属第三医院麻醉科，黑龙江省哈尔滨市  1500040
    4佳木斯大学，黑龙江省佳木斯市  154002
  • 收稿日期:2011-03-14 修回日期:2011-04-30 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 王健平，硕士，主任医师，佳木斯大学附属口腔医院牙体牙髓病一科，黑龙江省佳木斯市 154002 WJP.1959@ 163.com
  • 作者简介:张强★，男，1984年生，山西省长治市人，汉族，2011年佳木斯大学毕业，硕士，主要从事口腔基础医学方面的研究。 Sky9100101399@136.com

Biocompatibility of pure titanium implant surface micro-arc oxidation coating

Zhang Qiang1, Wang Jian-ping2, Hu Mei-ling3, Meng Xiang-cai4, Wang Jing4   

  1. 1Department of Prosthodontics, Second People’s Hospital of Changzhi City, Changzhi  046000, Shanxi Province, China
    2First Department of Pulp Disease, Stomatological Hospital of Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
    3Department of Anesthesiology, Third Affiliated Hospital of Harbin Medical University, Harbin  150040, Heilongjiang Province, China
    4Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
  • Received:2011-03-14 Revised:2011-04-30 Online:2011-12-17 Published:2011-12-17
  • Contact: Wang Jian-ping, Master, Chief physician, First Department of Pulp Disease, Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China WJP.1959@163.com
  • About author:Zhang Qiang★, Master, Department of Prosthodontics, Second People’s Hospital of Changzhi City, Changzhi 046000, Shanxi Province, China 13603695015@ 139.com

PDF

425

摘要：

背景：各种纯钛种植体表面微弧氧化涂层效果不尽相同。
目的：观察3种不同微弧氧化涂层种植体钛片对小鼠成骨细胞的细胞增殖、碱性磷酸酶活性和β1-integrin的基因表达水平的影响。
方法：采用国际常用小鼠成骨细胞系(MC3T3-E1)，3种不同涂层钛片作为影响因素，纯钛作为对照，采用MTT法和电镜法观察细胞附着和细胞增殖，PNPP法测定碱性磷酸酶的活性，RT-PCR法检测β1-integrin在小鼠成骨细胞中的表达。
结果与结论：MTT值、碱性磷酸酶值、β1-integrin的基因表达水平和电镜观察均显示含钙、磷、镁、锌元素的二氧化钛涂层钛片生物相容性最好，含钙磷盐的二氧化钛涂层钛片次之，二氧化钛涂层钛片最差。小鼠成骨细胞在其多孔，含有钙、磷、镁、锌元素表面的黏附及增殖最优。

关键词: 微弧氧化, 纯钛, 生物涂层, 成骨细胞, 生物相容性

Abstract:

BACKGROUND: Biological effects of various pure titanium implant surface micro-arc oxidation coatings are different.
OBJECTIVE: To study the effects of three different micro-arc oxidation coating palatal implant titanium tablets on mouse osteoblast cell proliferation, alkaline phosphatase activity and beta 1-integrin gene expression level.
METHODS: Using international common mice lines (MC3T3-E1), three different coated titanium pieces were used as influencing factors, pure titanium group as control group. MTT method and electron microscopic observation were conducted for cell adhesion and cell proliferation, PNPP was used for determination of alkaline phosphatase activity, RT-PCR method to detect beta 1-integrin expression in mouse osteoblasts.
RESULTS AND CONCLUSION: The MTT value, alkaline phosphatase activity, beta 1-integrin gene expression level and electron microscopic observation showed calcium, phosphorus, magnesium, zinc coated titanium pieces of titanium dioxide had the best biocompatibility, titanium dioxide coating titanium containing calcium containing phosphorus salt was secondary, and titanium dioxide coated titanium slice was the worst. The results showed that mouse osteoblasts on the porous of calcium, phosphorus, magnesium, zinc coated titanium pieces had the best adhesion and proliferative abilities.

中图分类号: