中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7734-7737.doi: 10.3969/j.issn.1673-8225.2011.41.033

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

SPF级Balb/c小鼠脏器质量、脏器系数、血常规、血生化指标的测定与比较

张修彦，詹纯列，肖育华，唐咸国   

  1. 广州军区广州总医院动物实验中心，广东省广州市  510010
  • 收稿日期:2011-07-21 修回日期:2011-08-22 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 詹纯列，主任医师，广州军区广州总医院动物实验中心，广东省广州市 510010 Zhanchunlie1963@sina.com
  • 作者简介:张修彦★，女，1986年生，汉族， 2010年华南理工大学毕业，硕士，主要从事动物模型研究。 449183654@qq.com
  • 基金资助:

    广东省科学技术厅科技基础条件建设项目(2008A060202016)

Measurement and comparisons of organ weight, organ coefficient, hematological parameters and hematological biochemical parameters of specific pathogen free Balb/c mice

Zhang Xiu-yan, Zhan Chun-lie, Xiao Yu-hua, Tang Xian-guo   

  1. Department of Medical Research, General Hospital of Guangzhou Military Command of PLA, Guangzhou   510010, Guangdong Province, China
  • Received:2011-07-21 Revised:2011-08-22 Online:2011-10-08 Published:2011-10-08
  • Contact: Zhan Chun-lie, Chief physician, Department of Medical Research, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China Zhanchunlie1963@ sina.com
  • About author:Zhang Xiu-yan★, Master, Department of Medical Research, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China 449183654@qq.com
  • Supported by:

    the National Science and Technology Infrastructure Program of the Ministry of Science and  Technology of Guangdong Province. No. 2008A060202016*

PDF

2840

被引次数

28

摘要：

背景：实验动物重要基础数据的标准化测定，是实行动物标准化监督管理的重要依据。
目的：测定SPF级Balb/c小鼠主要脏器质量、脏器系数、血常规、血生化指标，并进行比较。
方法：取18 g左右Balb/c小鼠240只，雌雄各半，饲养1周后，精确称量体质量和主要脏器质量，计算脏器系数，并检测血常规及血生化指标。
结果与结论：雌性与雄性Balb/c小鼠比较：①肺、脾、双肾、心、膀胱质量差异有显著性意义(P < 0.01或 P < 0.05)。②肺、脾、双肾、膀胱脏器系数差异有显著性意义(P < 0.01或 P < 0.05)。③白细胞、单核细胞、嗜碱性粒细胞百分比、红细胞、血红蛋白、红细胞平均血红蛋白浓度、红细胞分布宽度、嗜酸性粒细胞百分比差异有显著性意义(P < 0.01或 P < 0.05)。④总蛋白、白蛋白、白球比、血糖、肌酐、尿酸、三酰甘油、总胆固醇、低密度脂蛋白、高密度脂蛋白、铁、磷、尿素氮差异有显著性意义(P < 0.01或 P < 0.05)。说明性别对SPF级Balb/c小鼠主要脏器、主要脏器系数、血常规、血生化指标有影响。

关键词: 小鼠, 脏器质量, 血常规, 血生化, 性别

Abstract:

BACKGROUND: Measuring important basic data of experimental animals is the important basis for standardization and management of experimental animals.
OBJECTIVE: To measure and compare organ weight, organ coefficient, hematological parameters and hematological biochemical parameters of specific pathogen free (SPF) Balb/c mice.
METHODS: A total of 240 Balb/c mice were fed for 1 week and then organ weights, organ coefficient, hematological parameters and hematological biochemical parameters were measured.
RESULTS AND CONCLUSION: There were significant differences in weights of the heart, lung, spleen, double kidneys, and bladder between female and male Balb/c mice (P < 0.01 or P < 0.05). There were significant differences in organ coefficient of lung, spleen, double kidneys and bladder between female and male Balb/c mice (P < 0.01 or P < 0.05). There were significant differences in leukocyte, monocytes, basophils, monocytes (%), eosinophils, basophils (%), erythrocyte, hemoglobin, mean corpuscular hemoglobin concentration, and red blood cell distribution width between female and male Balb/c mice (P < 0.01 or    P < 0.05). There were significant differences in total protein, albumin, A/G, glucose, urea nitrogen, creatinine, uric acid, triglycerides, total cholesterol, low density lipoprotein, high density lipoprotein, iron, and phosphorus between female and male Balb/c mice(P < 0.01 or P < 0.05). These results suggest that gender has influences on organ weight, organ coefficient, hematological parameters and hematological biochemical parameters of SPF Balb/c mice.

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