中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7463-7466.doi: 10.3969/j.issn.1673-8225.2011.40.013

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐血间充质干细胞对T淋巴细胞旁分泌的免疫调节作用

徐丽南1，姚志成2，林  楠2，胡昆鹏2，钟跃思2   

  1. 1中山大学附属第一医院妇产科，广东省广州市 510080
    2中山大学附属第三医院肝胆外科，广东省广州市  510630
  • 收稿日期:2011-07-27 修回日期:2011-09-07 出版日期:2011-10-01 发布日期:2011-10-01
  • 作者简介:徐丽南☆，女，1977年生，河南省安阳市人，汉族，2011年中山大学毕业，博士，主治医师，主要从事围产医学的临床与基础研究。 drxulinan@gmail.com
  • 基金资助:

    国家自然科学青年基金(81000674)；广东省科技计划项目(2010B031600313)。

Immunoregulatory effect of human umbilical cord blood mesenchymal stem cells on proliferation of T lymphocytes through paracrine mechanism

Xu Li-nan1, Yao Zhi-cheng2, Lin Nan2, Hu Kun-peng2, Zhong Yue-si2   

  1. 1Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
    2Department of Hepatobiliary Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-07-27 Revised:2011-09-07 Online:2011-10-01 Published:2011-10-01
  • About author:Xu Li-nan☆, Doctor, Attending physician, Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China drxulinan@gmail.com
  • Supported by:

    the National Natural Science Foundation for the Youth, No. 81000674*; the Science and Technology Plan Program of Guangdong Province, No. 2010B031600313*

PDF

306

被引次数

5

摘要：

背景：相关研究表明脐血间充质干细胞具有一定的免疫调节作用，但具体机制不详。
目的：观察脐血源间充质干细胞通过旁分泌机制对T淋巴细胞增殖的影响。
方法：分离正常分娩产妇脐血间充质干细胞和健康志愿者外周血T淋巴细胞，按不同比例建立脐血间充质干细胞与T淋巴细胞非接触共培养体系，以单独培养T淋巴细胞作为对照组。
结果与结论：脐血间充质干细胞形态学呈现成纤维梭型细胞样，呈旋涡状团集生长。流式细胞仪检测脐血间充质干细胞表面标记物：CD29(+)，CD44(+)，CD34(-)，CD45(-)，HLA-DR(-)；与对照组相比，共培养组可明显抑制植物血凝素刺激T淋巴细胞的增殖作用(P < 0.05)，且呈剂量依赖性；ElISA法检测共培养组分泌的白细胞介素10水平较对照组明显升高(P < 0.05)；中和试验后脐血间充质干细胞对T淋巴细胞增殖的抑制作用明显减弱。结果说明脐血间充质干细胞可明显抑制异体外周血T淋巴细胞的增殖，可能是通过旁分泌白细胞介素10达到负向免疫调节作用。

关键词: 免疫调节, 脐血间充质干细胞, T淋巴细胞, 旁分泌, 白细胞介素10

Abstract:

BACKGROUND: Studies have shown that umbilical cord blood mesenchymal stem cells (UCB-MSCs) have a certain role in immune regulation, but the exact mechanism is unknown.
OBJECTIVE: To study the effect of UCB-MSCs on T lymphocytes by paracrine mechanism.
METHODS: We isolated the UCB-MSCs and T lymphocytes from the umbilical cord blood and peripheral blood. Indirect coculture system of UCB-MSCs and T lymphocytes with different proportion was constructed. The group of coculture acted as experiment group, the group of monoculture T lymphocytes as control group.
RESULTS AND CONCLUSION: UBC-MSCs presented a shuffle-type growth which liked the fibroblast in morphology; the flow cytometry showed the results of cellular surface marks: CD29(+), CD44(+), CD34(-), CD45(-), HLA-DR(-). Compared with the control group, the experiment group could dramatically inhibit the proliferation of peripheral blood T lymphocytes stimulated by phytohemagglutinin in a dose-dependent manner (P < 0.05); the concentration of interleukin-10 secreted by UCB-MSCs was significantly higher in the experiment group than the control group (P < 0.05). The ability of UBC-MSCs to inhibit the proliferation of T lymphocytes was sharply impaired by neutralization experiments. UCB-MSCs can dramatically inhibit the proliferation of peripheral blood T lymphocytes, which may depend on paracrine interleukin-10 to play an immunoregulatory role.

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