中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7085-7089.doi: 10.3969/j.issn.1673-8225.2011.38.013

• 药物控释材料 drug delivery materials • 上一篇    下一篇

幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性

刘  微1,2，谢  勇2，刘志军1   

  1. 1新余市人民医院消化内科，江西省新余市 338025
    2南昌大学第一附属医院消化疾病研究所，江西省消化疾病研究重点实验室，江西省南昌市  330006
  • 收稿日期:2011-02-05 修回日期:2011-03-12 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 谢勇，教授，主任医师，南昌大学第一附属医院消化系病研究所，江西省消化疾病研究重点实验室，江西省南昌市 330006 xieyong_med@163.com
  • 作者简介:刘微★，男，1983年生，江西省新余市人，汉族，2010年南昌大学医学院毕业，硕士，医师，主要从事幽门螺杆菌感染免疫致病和免疫防治。 baibianfuliuwei@163.com
  • 基金资助:

    国家自然科学基金资助课题(30460052)。

Preparation and in vitro release characteristics of chitosan microspheres loading Helicobacter pylori whole cell protein antigen

Liu Wei1,2, Xie Yong2, Liu Zhi-jun1   

  1. 1Digestive System Department, People's Hospital of Xinyu, Xinyu  338025, Jiangxi Province, China
    2Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-02-05 Revised:2011-03-12 Online:2011-09-17 Published:2011-09-17
  • Contact: Xie Yong, Professor, Chief physician, Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China xieyong_med@163.com
  • About author:Liu Wei★, Master, Physician, Digestive System Department, People's Hospital of Xinyu, Xinyu 338025, Jiangxi Province, China; Institute of Digestive Disease, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China baibianfuliuwei@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30460052*

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摘要：

背景：不同方法制备出的幽门螺杆菌全菌蛋白抗原壳聚糖微球，其包裹率和控释效果也不同。
目的：探讨幽门螺杆菌全菌蛋白抗原壳聚糖微球的最佳制备方案，观察其体外释放特性。
方法：使用Berthold沉淀法制备壳聚糖微球，筛选最佳制备方案；使用扫描电镜及粒径分析仪观察壳聚糖微球的形态及粒径分布；冻干后的壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原，使用BCA蛋白定量试剂盒测量分析微球的抗原包裹率、包裹量及释放率。
结果与结论：从32种壳聚糖微球制备方案中筛选出了以海得贝壳聚糖为原料、冰乙酸的浓度为1%、硫酸钠为沉淀剂、pH值为5.0、不进行超声处理方案为最佳制备方案，扫描电镜示微球光滑圆整、致密，粒径分布在1.0~5.0 μm；抗原包裹率为79.92%，包裹量为16.47%；体外释放实验表明，总抗原释放率为20.39%，呈缓慢释放状态。

关键词: 壳聚糖, 幽门螺杆菌, 微球, 包裹, 释放

Abstract:

BACKGROUND: Chitosan microspheres loading Helicobacter pylori whole cell protein antigen prepared by different methods have different the package rates and controlled-release effects.
OBJECTIVE: To prepare chitosan microspheres loading Helicobacter pylori whole cell protein antigen and observe the release characteristics in vitro.
METHODS: Berthold precipitation method was used to prepare chitosan microspheres and the preparation was optimized; scanning electron microscope and particle size analyzer were used to observe the shape and size measurement of chitosan microspheres; the BCA Protein Assay Kit was used to measure the loading efficiency, loading capacity and releasing rate of microspheres.
RESULTS AND CONCLUSION: We chose the best preparation program of chitosan microspheres from 32 species preparation programs: the best preparation program was the material of sea shell chitosan, 1% concentration of acetic acid, sodium sulfate for precipitating agent and 5.0 pH value, non-use of ultrasonic treatment. SEM results showed that the microspheres presented with smooth round and dense, diameters ranged from 1.0 μm to 5.0 μm; Antigen loading efficiency was 79.92%, loading capacity was 16.47%; Release experiment in vitro showed that the total antigen releasing rate was 20.39%, showing a slow-release status.

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