中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6127-6130.doi: 10.3969/j.issn.1673-8225.2011.33.012

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

建立小型猪Ⅱ度根分叉缺损模型的可行性

陈  珊，杨军英，张盛炎，冯  磊   

  1. 中山大学附属第一医院口腔科，广东省广州市 510080
  • 收稿日期:2011-04-06 修回日期:2011-07-18 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 杨军英，硕士，副教授，中山大学附属第一医院口腔科，广东省广州市510080 aliceyang2006@126.com
  • 作者简介:陈珊★，女，1983年生，江苏省响水县人，汉族，2010年中山大学毕业，硕士，医师，主要从事牙体牙髓病及牙周病方面的研究。 chenshan00001@163.com
  • 基金资助:

    课题受广东省科技计划项目(2009B060700042)资助，项目名称：辛伐他汀局部给药缓释剂型的研制及其对牙槽骨重建的作用。

Feasibility to establish a mini-pig model of class II furcation defect

Chen Shan, Yang Jun-ying, Zhang Sheng-yan, Feng Lei   

  1. Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China
  • Received:2011-04-06 Revised:2011-07-18 Online:2011-08-13 Published:2011-08-13
  • Contact: Yang Jun-ying, Master, Associate professor, Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China aliceyang2006@ 126.com
  • About author:Chen Shan★, Master, Physician, Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China chenshan00001@ 163.com
  • Supported by:

    Science and Technology Plan Program of Guangdong Province, No.2009B060700042*

PDF

592

被引次数

4

摘要：

背景：牙槽骨缺损再生的前提是建立可有效评价牙周骨组织再生的实验性牙槽骨缺损动物模型，以往研究用于观察牙周组织病变的模型动物多选用狗，但操作难度大。
目的：观察利用小型猪建立Ⅱ度根分叉缺损动物模型的可行性。
方法：制备小型猪Ⅱ度根分叉缺损模型。将小型猪实验牙随机分为2组，模型组造模后不注射任何药物干预，对照组于造模后注射具有成骨作用的辛伐他汀凝胶。
结果与结论：建模8周后大体、病理切片观察结果均显示模型组根分叉区空虚，对照组见新生牙槽骨长入。对照组可见较成熟的纤维组织以及增生的血管、大量成骨细胞、骨细胞，而模型组可见增生血管及少量成骨细胞。结果证实，以小型猪构建Ⅱ度根分叉缺损模型是可行的。

关键词: 小型猪, Ⅱ度根分叉缺损, 动物模型, 辛伐他汀, 牙槽骨, 牙组织构建

Abstract:

BACKGROUND: The premise of an effective evaluation of periodontal tissue regeneration is to successfully establish alveolar bone defect model. Most of previous studies use dogs as experimental animals, but the operation is more difficult.
OBJECTIVE: To investigate the possibility of establishing an animal model of class Ⅱ furcation defect in mini-pigs.
METHODS: Mini-pig models of class Ⅱ furcation defect were prepared. The model group did not receive any agents while the control group was weekly injected with 2.2 mg simvastatin/methylcellulose gel.
RESULTS AND CONCLUSION: Eight weeks later, gross and pathological observation showed that it was empty in the defects of the model group and new bone was found in the control group. Mature fibrous tissue and proliferative vessels as well as a large number of osteoblasts and osteocytes were observed in the control group, while little new bone, proliferative vessels and a small number of osteoblasts were observed in the model group. These results showed that it is feasible to establish class Ⅱ furcation defect in mini-pigs.

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