中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4335-4338.doi: 10.3969/j.issn.1673-8225.2012.23.030

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

诱导脐血间充质干细胞向神经元样细胞分化

陈 镭1，陈 冰2，谢福堂1   

  1. 1天津市第五中心医院神经外科，天津市 300451；
    2上海市浦东新区南汇中心医院妇产科，上海市 201300
  • 收稿日期:2012-04-24 修回日期:2012-05-10 出版日期:2012-06-03 发布日期:2013-11-06
  • 作者简介:陈镭☆，男，1970年生，江苏省吴县人，汉族，2005年天津医科大学毕业，博士，副主任医师，主要从事颅底显微外科解剖和神经干细胞移植研究。 chenlei1130@163.com
  • 基金资助:

    天津市滨海新区塘沽科技发展专项(2011MS06-05)

Induced differentiation of umbilical cord blood mesenchymal stem cells into neuron-like cells

Chen Lei1, Chen Bing2, Xie Fu-tang1   

  1. 1Department of Neurosurgery, Tianjin Fifth Central Hospital, Tianjin 300451, China;
    2Department of Obstetrics and Gynecology, Nanhui Central Hospital, Pudong New Area, Shanghai 201300, China
  • Received:2012-04-24 Revised:2012-05-10 Online:2012-06-03 Published:2013-11-06
  • About author:Chen Lei☆, M.D., Associated chief physician, Department of Neurosurgery, Tianjin Fifth Central Hospital, Tianjin 300451, China Chenlei1130@163.com

PDF

420

被引次数

10

摘要：

背景：脐血来源的间充质干细胞具有与造血干细胞和骨髓间充质干细胞相似的多向分化潜能，并且可以诱导分化为神经元样细胞，但分离培养后的成功率比较低，需要寻求一种成功率较高的培养方法。
目的：探讨脐血间充质干细胞的生物学特性及向神经元样细胞诱导分化的方法。
方法：由作者检索CNKI数据库2002/2011收录的有关脐血间充质干细胞诱导分化为神经元样细胞的相关研究文献，中文检索词为“脐血间充质干细胞，神经细胞，诱导分化，细胞因子”。
结果与结论：脐血间充质干细胞具有与造血干细胞和骨髓间充质干细胞相似的多向分化潜能，可以在体外诱导分化为神经样细胞，对CD34表达阴性，是区别于脐血造血干细胞的主要标志物。脐血间充质干细胞在体外诱导分化的方法主要有细胞生长因子法、化学诱导剂法、生长因子与诱导剂联合法，通过各种神经诱导剂的作用，使培养出的神经元样细胞数量有所增加，国内外基础研究结果显示移植脐血间充质干细胞诱导分化的神经元样细胞可以修复中枢神经系统损伤性疾病，脐血间充质干细胞是神经损伤修复的一种理想种子细胞。

关键词: 脐血间充质干细胞, 神经细胞, 诱导分化, 神经元样细胞, 细胞因子, 干细胞

Abstract:

BACKGROUND: Umbilical cord blood-derived mesenchymal stem cells have similar multipotential differentiation to hematopoietic stem cells and bone marrow mesenchymal stem cells, and can be induced to differentiate into neuron-like cells. However, the success rate of isolation and culture is very low. So it is necessary to search a method for culture of umbilical cord blood-derived mesenchymal stem cells with higher success rate.
OBJECTIVE: To explore the biological characteristics of umbilical cord blood-derived mesenchymal stem cells and to search a method for induced differentiation into neuron-like cells.
METHODS: A computer-based online retrieval of CNKI database was performed to search papers describing induced differentiation of umbilical cord blood-derived mesenchymal stem cells into neuron-like cells published between 2002-2011 using the key words “umbilical cord blood-derived mesenchymal stem cells”, “neural cells”, “induced differentiation” and “cytokines”.
RESULTS AND CONCLUSION: Umbilical cord blood-derived stem cells show similar multipotential differentiation to hematopoietic stem cells and bone marrow mesenchymal stem cells and can be induce-differentiated into neuron-like cells. They are negative for CD34 and therefore are the main marker for differentiating hematopoietic stem cells and bone marrow mesenchymal stem cells. The methods for in vitro induced differentiation of umbilical cord blood-derived mesenchymal stem cells include cell growth factor method, chemical inducers method, growth factors combined with inducers method. Research from China and abroad shows that neuron-like cells induce-differentiated by umbilical cord blood-derived mesenchymal stem cells can be used for treatment of central nervous system diseases, and umbilical cord blood-derived mesenchymal stem cells are a kind of ideal seed cells for repair of nerve injury.

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