中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2583-2586.doi: 10.3969/j.issn.1673-8225.2011.14.024

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

不同浓度淫羊藿含药血清干预骨髓基质干细胞的成脂分化

刘洪江1，刘海全1，秦佳佳2   

  1. 1广州中医药大学附属骨伤科医院，广东省广州市 510405
    2暨南大学，广东省广州市 510632
  • 收稿日期:2011-01-04 修回日期:2011-03-05 出版日期:2011-04-02 发布日期:2013-11-02
  • 通讯作者: 刘海全，副研究员，广州中医药大学附属骨伤科医院，广东省广州市510405
  • 作者简介:刘洪江★，男，1978年生，河南省洛阳市人，汉族，2005年广州中医药大学毕业，硕士，主治中医师，主要从事中医药防治骨质疏松的临床和科研研究。 gdgzhlhj@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30901915)；中国博士后科学基金面上资助项目(20080440744)。

Adipogenic differentiation of bone marrow stromal stem cells intervened with serum containing different concentration Epimedium 

Liu Hong-jiang1, Liu Hai-quan1, Qin Jia-jia2   

  1. 1Orthopedic Hospital, Guangzhou University of Chinese Medicine, Guangzhou  510405, Guangdong Province, China
    2Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-04 Revised:2011-03-05 Online:2011-04-02 Published:2013-11-02
  • Contact: Liu Hai-quan, Associate investigator, Orthopedic Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • About author:Liu Hong-jiang★, Master, Attending physician, Orthopedic Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China gdgzhlhj@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30901915*; China Postdoctor Science Foundation, No. 20080440744*

PDF

609

被引次数

25

摘要：

背景：如何抑制骨髓基质干细胞成脂分化，促进其成骨分化将是防治骨质疏松的新途径之一。
目的：观察不同浓度淫羊藿含药血清对骨质疏松大鼠骨髓基质干细胞成脂分化的干预作用。
方法：全骨髓培养法培养SD大鼠骨髓间充质干细胞，12只SPF级SD雌性大鼠按体表面积的方法给予淫羊藿水提液，药物以蒸馏水定容至所需浓度，相当于临床剂量的20倍、10倍、5倍灌胃，连续灌胃7 d后眼眶取血，观察各组干预后7 d成脂分化情况。
结果与结论：中药低、中剂量含药血清组的成脂率低于空白组 (P < 0.01、0.05)，说明中药淫羊藿含药血清发挥了抑制骨质疏松骨髓基质干细胞向脂肪细胞分化的作用。中药各剂量组的组间比较差异无显著性意义(P  > 0.05)，但3组成脂率数值方面以中剂量组最佳。

关键词: 淫羊藿, 含药血清, 骨髓基质干细胞, 成脂分化, 骨质疏松

Abstract:

BACKGROUND: How to inhibit adipogenic differentiation of bone marrow stromal stem cells (BMSCs) and promote the osteogenic differentiation of BMSCs is a new approach to prevention and treatment of osteoporosis.
OBJECTIVE: To investigate the effect of serum containing different concentration Epimedium on adipogenic differentiation of BMSCs in rats with osteoporosis.
METHODS: Three of 15 SPF-class SD female rats were used to isolate and culture BMSCs. The remaining 12 rats were divided into control group, high dose herb group, medium dose herb group, low dose herb group. Adipogenic differentiation was observed after 7 day’s intervention.
RESULTS AND CONCLUSION: Medium and low dose herb groups played the effects on suppressing adipogenic differentiation of BMSCs (P < 0.01, 0.05). There was no significant difference among each herb groups (P > 0.05). The medium dose herb was the best in different concentration dose herb on inhibiting adipogenic differentiation of BMSCs.

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