中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1241-1244.doi: 10.3969/j.issn.1673-8225.2011.07.022

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

七叶洋地黄双苷滴眼液对翼状胬肉成纤维细胞生长分化的影响

张立贵1,刘海霞2,曲延刚3   

  1. 1山东省沪滨眼科医院,山东省滨州市256600
    2中国海洋大学信息学院,山东省青岛市 226100
    3湖北恩施州中心医院病理科,湖北省恩施市445000
  • 收稿日期:2010-08-04 修回日期:2010-12-14 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 张立贵☆,1962年生,山东省费县人,1986年青岛医学院毕业,博士,副主任医师,副教授。 Liguizz@163. com
  • 作者简介:张立贵☆,1962年生,山东省费县人,1986年青岛医学院毕业,博士,副主任医师,副教授。 Liguizz@163. com

Influence of Esculin and Digitalisglycosides Eye Drops on the proliferation and differentiation fibroblasts from pterygium

Zhang Li-gui1, Liu Hai-xia2, Qu Yan-gang3   

  1. 1Hubin Ophthalmological Hospital, Binzhou  256600, Shandong Province, China
    2Infomation Science and Engineering College, Ocean University of China, Qingdao  266100, Shandong Province, China
    3Department of Pathology, Central Hospital of Enshi, Enshi  445000, Hubei Province, China
  • Received:2010-08-04 Revised:2010-12-14 Online:2011-02-12 Published:2011-02-12
  • Contact: Zhang Li-gui, Doctor, Associate chief physician, Associate professor, Hubin Ophthalmological Hospital, Binzhou 256600, Shandong Province, China Liguizz@163.com
  • About author:Zhang Li-gui☆, Doctor, Associate chief physician, Associate professor, Hubin Ophthalmological Hospital, Binzhou 256600, Shandong Province, China Liguizz@163.com

摘要:

背景:已有研究证实七叶洋地黄双苷滴眼液滴眼液能够促进组织的血供,但在治疗黄斑变性的同时是否加速翼状胬肉的生长却不清楚。
目的:观察七叶洋地黄双苷滴眼液对翼状胬肉成纤维细胞生长分化的影响。
方法:纳入同时有黄斑变性和翼状胬肉的患者26例,一眼给予七叶洋地黄双苷滴眼液点眼,随访时间为3,6,12,18和24个月,每次皆收集翼状胬肉图像并观察其翼状胬肉侵蚀角膜面积变化。最后8例行翼状胬肉手术治疗,切除的组织行成纤维细胞体外培养,ELISA法检测成纤维细胞基质金属蛋白酶1,3,9的表达,荧光定量PCR法检测成纤维细胞基质金属蛋白酶3 mRNA的表达。同时找年龄匹配的未用药的8例初发翼状胬肉组织作对照,并与年龄匹配的正常结膜组织对比。
结果与结论:患者用七叶洋地黄双苷滴眼液治疗后翼状胬肉侵蚀角膜的面积生长速度明显加快( < 0.01),而且其翼状胬肉成纤维细胞中基质金属蛋白酶1,3水平较对照组和正常结膜组织均增加(P < 0.01),基质金属蛋白酶3 mRNA的表达比对照组高了52.7% ( < 0.01)。结果提示,七叶洋地黄双苷能促进翼状胬肉的增长,其机制可能与其促进翼状胬肉组织中成纤维细胞基质金属蛋白酶1,3的分泌,促进基质金属蛋白酶3 mRNA的表达有关。

关键词: 七叶洋地黄双苷滴眼液, 翼状胬肉, 基质金属蛋白酶, 成纤维细胞, 组织构建

Abstract:

BACKGROUND: Studies have confirmed Esculin and Digitalis glycosides Eye Drops can improve tissue blood supply, while it remains unclear whether they accelerate the growth of pterygium in curing macular denaturation.
OBJECTIVE: To observe the effect of Esculin and Digitalis Glycosides Eye Drops on the proliferation of the fibroblasts of pterygium. 
METHODS: A total of 26 patients with pterygium in double eyes and macular denaturation in one eye were selected, and treated with Esculin and Digitalisglycosides Eye Drops. They were followed up for 3, 6, 12, 18 and 24 months. Images of pterygium were collected to observe the areas changing of prerygium. The pterygium in 8 patients was operated, and the fibroblasts from the removed tissues were cultured. Matrix metalloproteinase (MMP)-1, 3, 9 were determined by ELISA method and MMP-3 mRNA was detected by real-time fluorescent quantitative PCR method. In addition, the tissues from age-matched patients with primary pterygium and normal conjunctiva tissues from patients with cataract were used as control.
RESULT AND CONCLUSION: Following treatment with Esculin and Digitalisglycosides Eye Drops, the areas of the pterygium erosion cornea grew obviously rapidly ( < 0.01). The contents of MMP-1 and MMP-3 of the fibroblasts of Esculin and Digitalisglycosides Eye Drops group were higher than that of control group and normal conjunctiva tissues ( < 0.01), and the expression of MMP-3 mRNA was 52.7% greater than that of control group (P < 0.01). Results indicated that Esculin and Digitalisglycosides Eye Drops can promote the growth of pterygium, probably by promoting the secreting of MMP-1, 3 and promoting the expression of MMP-3 mRNA in fibroblasts.

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