中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1245-1248.doi: 10.3969/j.issn.1673-8225.2011.07.023

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

Bcl-xL基因在正常皮肤血管组织、增生及退化期血管瘤组织中的表达

游  浩1，高劲松2，陈方舟1，朱胜文1，吴  磊1   

  1. 1武汉市第五医院，江汉大学第二附属医院骨科，湖北省武汉市430050
    2长沙医学院基础部，湖南省长沙市410219
  • 收稿日期:2010-09-05 修回日期:2010-12-09 出版日期:2011-02-12 发布日期:2011-02-12
  • 作者简介:游浩★，男， 1969年生，硕士，湖北省人，2006年武汉大学医学院毕业，汉族，副主任医师，主要从事研究工作脊柱疾病和骨肿瘤的研究。 yourenyouyu2010@yahoo.cn
  • 基金资助:

    课题受国家自然科学基金(30271345)，湖北省科技厅(2004AA301c107)和湖北省卫生厅(JXIB075)资助。

Expression of Bcl-xL gene in normal skin and the proliferating and degenerating hemangioma tissues

You Hao1, Gao Jin-song2, Chen Fang-zhou1, Zhu Sheng-wen1, Wu Lei1   

  1. 1Department of Orthopedics, Second Affiliated Hospital of Jianghan University, Wuhan Fifth Hospital, Wuhan  430050, Hubei Province, China
    2Department of Basic Medicine,  Changsha Medical College, Changsha  410219, Hunan Province, China
  • Received:2010-09-05 Revised:2010-12-09 Online:2011-02-12 Published:2011-02-12
  • About author:You Hao★, Master, Associate chief physician, Department of Orthopedics, Second Affiliated Hospital of Jianghan University, Wuhan Fifth Hospital, Wuhan 430050, Hubei Province, China yourenyouyu2010@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30271345*; the Science and Technology Department of Hubei Province, No. 2004AA301c107*; the Health Department of Hubei Province, No. JXIB075*

PDF

363

被引次数

3

摘要：

背景：有文献报道Bcl-xL基因能抑制细胞凋亡，并可能参与血管形成，但目前Bcl-xL基因在恶性肿瘤中的研究较多，而在血管瘤的研究较少。
目的：应用免疫组织化学染色法检测增生期和退化期血管瘤和正常皮肤血管组织中Bcl-xL基因的表达。
方法：收集有完整临床和病理资料的40例皮肤血管瘤手术切除标本，包括增生期血管瘤22例，退化期血管瘤18例。另取瘤组织周围正常皮肤组织5例作为对照。采用免疫组织化学染色方法检测Bcl-xL在各组中的表达，并结合Ⅷ因子相关抗原的免疫组织化学染色证实表达Bcl-xL阳性表达的细胞为血管瘤组织中的内皮细胞，并测定Bcl-xL在各组中的平均吸光度和平均阳性面积率。
结果与结论：增生期血管瘤内皮细胞胞浆内可见较多的棕黄色颗粒沉积，Bcl-xL表达呈强阳性；退化期血管瘤和正常皮肤组织内皮细胞胞浆内可见少量的棕黄色颗粒或无棕黄色颗粒，Bcl-xL表达弱或无表达。增生期血管瘤与退化期血管瘤和正常皮肤组相比，Bcl-xL阳性表达的平均吸光度和阳性面积率明显增高(P < 0.01)。结果显示，Bcl-xL为抗凋亡基因，在增生期时呈高表达，其促进了血管内皮细胞的增殖，参与了血管瘤的增生。

关键词: 血管瘤, Bcl-xL基因, 凋亡, 免疫组织化学, 组织构建

Abstract:

BACKGROUND: Bcl-xl gene can suppress cell apoptosis and may participate in angiopoiesis. However, more studies concerning Bcl-xL gene in malignant tumor, few are reported in hemangioma. 
OBJECTIVE: To study the expression of Bcl-xL gene in normal skin vessels, hyperplasia and degeneration hemangioma tissues using immune-histochemical technique.
METHODS: Specimens were obtained from 40 cases of dermal hemangioma by exairesis, comprising 22 cases with proliferating hemangiomas and 18 with degenerating hemangiomas. In addition, normal skin tissues around hemangiomas from 5 cases were also chosen for controls. Immunohistochemical stainings were performed to detect the expression of Bcl-xL in those three groups. Endothelial cells were identified by expressing factor Ⅷ-related antigen. The average absorbance and the rate of positive area of expression of Bcl-xL were analyzed.
RESULTS AND CONCLUSION: There were plenty of brown particles in the cytoplasm of proliferating endothelial cells, which strongly positive expressed Bcl-xL. There were few and mo brown particles in endothelial cells cytoplasm of the degenerating hemangiomas or normal skins. The expression of Bcl-xL were weakly and no expression. Compared with the normal skins, the average absorbance and rate of Bcl-xL positive expression were higher in the proliferative and degenerating hemangiomas (P < 0.01). Bcl-xL is an anti-apoptosis gene and was highly expressed in the proliferative phase, which promoted proliferation of blood vessel endothelial cells and participated in hyperplasia of hemangiomas.

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