[1]	李 黎, 马 力. 磁性壳聚糖微球固定化乳糖酶及其酶学性质[J]. 中国组织工程研究, 2021, 25(4): 576-581.
[2]	李鑫平, 崔秋菊, 曾曙光, 冉高英, 张兆强, 刘显文, 方 炜, 徐帅妹. β-磷酸三钙/壳聚糖水凝胶改性对牙髓干细胞生长与矿化的影响[J]. 中国组织工程研究, 2021, 25(22): 3493-3499.
[3]	刘振东, 王 瑞, 李小磊, 王静成. 干扰素α-2b抑制瘢痕形成的研究与热点[J]. 中国组织工程研究, 2021, 25(2): 317-321.
[4]	陈思宇, 李燕楠, 颉丽英, 刘司麒, 范玉蓉, 房昌星, 张 鑫, 权家宇, 左 琳. 负载碱性成纤维细胞生长因子温敏性壳聚糖-胶原复合水凝胶可减缓小鼠心肌梗死后心室的重构[J]. 中国组织工程研究, 2021, 25(16): 2472-2478.
[5]	刘 冯, 张 瑜, 王燕丽, 骆 威, 韩超珊, 李杨欣. 温敏型壳聚糖水凝胶包封外泌体在缺血性疾病中的应用[J]. 中国组织工程研究, 2021, 25(16): 2479-2487.
[6]	李 洁, 许楗桢, 胡 萍, 雷琪琪, 张文柠, 敖宁建. 用于慢性伤口羧甲基壳聚糖/氧化葡甘聚糖/三七总皂苷复合海绵的制备及性能评价[J]. 中国组织工程研究, 2021, 25(16): 2528-2534.
[7]	封小霞, 侯玮玮, 金晓婷, 王心华. 构建聚乳酸-羟基乙酸电纺丝-壳聚糖电喷微球牙周仿生膜[J]. 中国组织工程研究, 2020, 24(4): 511-516.
[8]	李 黎, 马 力, 李 鹤. 磁性壳聚糖微球的制备及表征[J]. 中国组织工程研究, 2020, 24(4): 577-582.
[9]	刘海燕, 胡 阳, 武秀萍, 潘浩波, 荆 璇. 壳聚糖基多糖材料防治口腔疾病的作用[J]. 中国组织工程研究, 2020, 24(4): 631-636.
[10]	廖 健, 黄晓林, 霍 花, 周 倩, 程余婷, 齐昱晗, 伍 超, 杨童景, 廖运茂, 梁 星. 煅烧骨/壳聚糖复合材料对成骨细胞增殖黏附的影响[J]. 中国组织工程研究, 2020, 24(34): 5447-5453.
[11]	方妤露, 易兵成, 沈炎冰, 唐 寒, 张彦中. 玉米壳纤维增强壳聚糖基水凝胶应用于软骨组织工程支架的潜力[J]. 中国组织工程研究, 2020, 24(34): 5493-5501.
[12]	廖 健, 黄晓林, 周 倩, 霍 花, 齐昱晗, 伍 超, 石前会, 杨童景, 廖运茂, 梁 星. 煅烧骨/壳聚糖复合材料促进SD大鼠骨髓间充质干细胞的成骨分化[J]. 中国组织工程研究, 2020, 24(31): 4941-4947.
[13]	李振想, 姜孝奎, 申芳芳, 李韶山. 结直肠癌细胞来源外泌体对CD8+T细胞的免疫调节[J]. 中国组织工程研究, 2020, 24(31): 5002-5006.
[14]	王德欣, 许战武, 裴国献. 骨髓间充质干细胞在淫羊藿苷/羟基磷灰石/聚乳酸-羟基乙酸共聚物支架上的成骨[J]. 中国组织工程研究, 2020, 24(25): 3974-3980.
[15]	廖 健, 黄晓林, 周 倩, 程余婷, 霍 花, 李 芳, 伍 超, 石前会, 廖运茂, 梁 星. 煅烧骨/壳聚糖复合材料的制备及表征[J]. 中国组织工程研究, 2020, 24(22): 3452-3459.

One of the most common factors of the failed back surgery syndrome (FBSS) after laminectomy is the epidural scar formation[1-3]. Therefore, how to prevent postoperative epidural scar has always been one of the key research topics, while the application of epidural barrier materials is one of these hot studies. The epidural barriers include autologous tissue transplant (such as autologous free fat[4-5]), traditional barrier materials (such as silica gel and glutin sponge), polymer materials (such as polylatic acid (PLA) and polyglycolic acid (PGA)[6-7]), and semiliquid anti-adhesion materials (such as hyaluronic acid and chitosan[8-9]).
In recent years, many studies regarding poly (lactic-co-glycolic acid) (PLGA) as a hot biological material have been performed in various aspects.
Compared with other materials, besides the good biocompatibility, biodegradability and good mechanical properties, the degradation time of PLGA could be changed through adjusting the proportion of PLA and PGA according to various requirements. In the present study, two composite membranes were prepared by PLGA combined with interferon (IFN) α-2b and chitosan respectively, which were used in laminectomy animal models and were compared with other materials. The feasibility of these two composite membranes preventing epidural scar adhesion after laminectomy was explored.

In recent years, the biodegradable synthetic polymer materials were universally concerned and applied[11], in which the PLGA was well studied. No apparent disintegration was observed during the biodegration of PLGA. PLGA, as a hot biological material, has been studied from various aspects. The PLGA has been successfully used as a carrier material of delayed released system for protein medicine[12-15]. Several studies have been performed addressing the effects of synthetic polymer materials in preventing epidural scar adhesion after laminectomy and satisfactory results have been achieved[16-17]. However, the single membrane implantation can play a positive role in isolating from epidural scar, but it exhibits weak capacity to inhibit fibroblasts, so it can not fundamentally decrease the epidural scar and adhesion. For this reason, the membrane combined with one drug with the potential to inhibit fibroblast growth without toxicity and side effects would exhibit the ability of mechanical barrier and inhibiting fibroblast growth. Therefore, this kind of composite membrane theoretically can prevent epidural scar adhesion very well after laminectomy.
In recent years, IFN has been used in clinical treatments of some diseases related to fibrosis owing to its broad-spectrum anti-fibrosis function. The mechanisms underlying IFN inhibiting scar formation might be to antagonize some cytokines directly or indirectly, inhibit fibroblast proliferation, chemotaxis and collagen synthesis[18-19]. Chitosan, an absorable, degradable biomaterial with good histocompatibility, has antibacterial, antimycotic potentials as well as hemostasic functions, can promote epithelial cell growth and inhibit fibroblast growth and adhesion[20-22].
Results from this study demonstrated that a single PLGA membrane has a good ability of barrier from the scar tiisue, which was similar to the autologous free fat transplantation. There was significant difference in gross observation scores between the PLGA, autologous free rat groups and the control group. Sodium hyaluronate showed better ability of inhibitting epidural scar adhesion in the early stage, while in the later stage, there was much fibrosis scar formation, but which was still less than that in the control group. Results from this study also revealed that IFN/PLGA and chitosan/PLGA composite membranes could effectively inhibit epidural scar formation and reduce adhesion to dura mater. There was significant difference in gross observation scores between these two composite membrane groups and other groups. But no significant difference was found between these two composite membrane groups. TEM results revealed that the fibroblasts had uniform chromatin, abundant endoplasmic reticulum with slight expansion in the control, autologous free fra, and PLGA groups. These findings suggest that the fibroblasts were in good condition and had an active function. In the sodium hyaluronate group, a little rough endoplasmic reticulum without obvious expansion in fibroblasts was found, and there was the phenomenon of mitochondrial vacuolation. In the IFN/PLGA and chitosan/PLGA groups, there were a few fibroblasts with irregular nucleus, a little rough endoplasmic reticulum with expansion, mitochondrial swelling, vacuolation, shorted or absent cristae. These findings indicate that the fibroblasts were in bad condition and showed poor functions.
Taken together, results from this study demonstrated that IFN/PLGA and chitosan/PLGA composite membranes not only play a role in isolating from fibrosis scar, but also have the ability of inhibiting fibroblast growth, suggesting that these two composite membranes are effective artificial materials to prevent epidural scar adhesion after laminectomy.

	阅读次数
	全文
	摘要