中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (26): 4751-4754.doi: 10.3969/j.issn.1673-8225.2010.026.001

• 数字化骨科 digital orthopedics •    下一篇

适于推拿手法细胞力学加载装置模型的设计与构建

马惠昇1，张 宏2，严隽陶2，沈国权2    

  1. 1宁夏医科大学中医学院，宁夏回族自治区银川市  750004；
    2上海中医药大学附属岳阳中西医结合医院，上海市  200437
  • 出版日期:2010-06-25 发布日期:2010-06-25
  • 通讯作者: 张宏，博士，副主任医师，上海中医药大学附属岳阳中西医结合医院，上海市 200437
  • 作者简介:马惠昇，男，1970年生，宁夏回族自治区贺兰县人，回族，2007年上海中医药大学毕业，博士，副教授，主要从事推拿手法基础与临床研究。 madoctor@163. com
  • 基金资助:

    国家自然基金资助项目（30300462）

Design and construction of loading equipment suitable to manipulation for cell mechanics

Ma Hui-sheng1, Zhang Hong2, Yan Jun-tao2, Shen Guo-quan2   

  1. 1Department of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan   750004, Ningxia Hui Autonomous Region, China;
    2Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai   200437, China
  • Online:2010-06-25 Published:2010-06-25
  • Contact: Zhang Hong, Doctor, Associate chief physician, Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • About author:Ma Hui-sheng, Doctor, Associate professor, Department of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China madoctor@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30300462*

PDF

554

被引次数

20

摘要：

背景：目前对推拿手法作用机制的研究多着眼于软组织、骨关节、神经、血管等宏观层面，从微观细胞学水平进行研究的鲜有报道。
目的：研究一种适用于推拿手法进行体外细胞力学加载实验的装置，为在细胞学水平开展手法作用研究寻找新的途径和方法。
方法：模拟血管内皮细胞在血管内壁的分布形式，将人脐静脉内皮细胞接种在自制弹性培养管内培养成活，建立了与血管弹性相似的血管模型，用与肌肉弹性系数相同的弹性海绵材料模拟血管外组织，将血管模型置入模拟组织中间，在模拟组织表面直接实施手法，进而刺激模拟血管中的人脐静脉内皮细胞，最大限度地再现细胞在体内的受力环境。
结果与结论：运用弹性软体血管模型，成功建立了适于推拿手法的细胞力学加载模型。该模型的建立，解决了宏观手法力无法对微观细胞进行加载的关键问题，为研究推拿手法的细胞分子作用机制开辟了新的途径。

关键词: 推拿, 手法, 人脐静脉内皮细胞, 细胞力学模型, 作用机制, 数字化医学

Abstract:

BACKGROUND: Studies on the action mechanisms of manipulation mostly focus on the field in soft tissue, bone joint, nerve, and blood vessel. There are few reports on cytological level.
OBJECTIVE: To design one kind of loading equipment suitable to manipulation for cell experiment in vitro to provide novel pathway and method for effective researches in cytological level.
METHODS: Vascular endothelial cells distribution in vessel wall was simulated. Human umbilical vein endothelial cells (HUVECs) were planted into elastic cultivated tube which was regarded as model of blood vessel. Then elastic sponge materials with elasticity coefficient similar to muscle were used to simulate the tissues around the vessels and blood vessel models were placed in the simulated tissue. Rolling manipulation was operated on the surface to stimulate HUVECs to simulate in vivo mechanical environment to the largest extent.
RESULTS AND CONCLUSION: A loading model for cell mechanics in vitro suitable to manipulation was established. The establishment of this model provides a new way to investigate the mechanism of manipulation action in the cytobiology.

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